Pathology classification of nasopharyngeal carcinoma

Sharif, Sharifah Emilia Tuan; Zawawi, Norzaliana; Yajid, Aidy Irman; Shukri, Norasnieda Md; Mohamad, Irfan

doi:10.1016/b978-0-12-814403-9.00005-7

Cited by 2 publications

(3 citation statements)

References 17 publications

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“…1,2 Environment and diet are important to influence factors in the development of NPC. 3,4 In the treatment of NPC, the improvement of intensive radiotherapy has significantly improved the survival rate of patients with NPC. 5,6 The purpose of this study was to investigate the molecular mechanism of NPC progression and to further improve the prognosis of NPC patients.…”

Section: Introductionmentioning

confidence: 99%

Circ_0000285 regulates nasopharyngeal carcinoma progression through miR-1278/FNDC3B axis

Zeng

et al. 2023

Hum Exp Toxicol

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Background Nasopharyngeal carcinoma (NPC) is cancer with high mortality and poor prognosis. Circular RNAs (circRNAs) have been identified in a wide variety of cancers. But the functional mechanism of circ_000285 in NPC remains unclear. Purpose To decipher the biological function and molecular mechanism of circ_000285 in NPC. Methods Quantitative PCR (RT-qPCR) was applied for detecting the expression of circ_0000285, miR-1278, and FNDC3B. Western blot was used to measure the protein levels of Fibronectin type III domain containing 3B (FNDC3B), Bcl2 associated X (Bax), and B cell leukemia/lymphoma 2 (Bcl2). Cell proliferation, migration, and invasion were analyzed by colony formation, 5-ethynyl-2′-deoxyuridine (EdU), and transwell assays. Cell apoptosis was detected by flow cytometry assays. ELISA assay was used to analyze Caspase-3 activity. Bioinformatics was used to predict, and the target relationship between miR-1278 and circ_0000285 or FNDC3B was verified by luciferase reporter assay. Tumor xenograft models were established to examine how circ_0000285 functions during the mediation of NPC tumor growth in vivo. Results Increased circ_0000285 and FNDC3B expressions, and a decreased miR-1278 expression were observed in NPC tissues and cell lines. Knockdown of circ_0000285 inhibited NPC cell proliferation, migration, invasion, and while promoting NPC cell apoptosis in vitro. Circ_0000285 knockdown-mediated anti-tumor effects in NPC cells could be largely reversed by silencing of miR-1278 or overexpression of FNDC3B. Circ_0000285 could up-regulate FNDC3B expression by sponging miR-1278 in NPC cells. Knockdown of circ_0000285 could inhibit tumor growth in vivo. Conclusion Circ_0000285 upregulates FNDC3B expression by adsorbing miR-1278 to promote NPC development.

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Section: Introductionmentioning

confidence: 99%

Circ_0000285 regulates nasopharyngeal carcinoma progression through miR-1278/FNDC3B axis

Zeng

et al. 2023

Hum Exp Toxicol

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“…The WHO's classifications are as follows: The creation of keratin protein distinguishes keratinising squamous cell carcinoma, also known as type 1, differentiated non-keratinising carcinoma, generally known as type 2, and undifferentiated carcinoma, popularly known as type 3 (2)(3)(4). Type 1 NPC exhibits keratinisation characteristics with intercellular bridges and squamous histologically (5). This sort of NPC is described as poorly or moderately differentiated.…”

Section: Introductionmentioning

confidence: 99%

“…This sort of NPC is described as poorly or moderately differentiated. Types 2 and 3 are more prevalent and strongly associated to the Epstein-Barr virus (EBV), especially in Asia, while type 1 is uncommon in areas where NPC is endemic (5,6). Similar to undifferentiated NPCs in terms of histological appearance, nonkeratinising differentiated NPCs have former cell borders that are stratified and pavemented (3).…”

Section: Introductionmentioning

confidence: 99%

Preliminary Analysis of Stim-1 Expression on Formalin-Fixed Paraffin-Embedded Nasopharyngeal Cancer Tissues

Okekpa,

S. M. N. Mydin,

Saravanan

et al. 2023

JUMMEC

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Nasopharyngeal cancer (NPC) is commonly diagnosed at the advanced stage with a poor prognosis. STIM-1 has become a promising cancer biomarker, especially in early diagnostic applications. There is a limited study on STIM1 expression in NPC, especially in formalin-fixed paraffin-embedded NPC tissues. Thus, the present work provides a preliminary analysis of STIM-1 expression on NPC tissues. This study aims to examine STIM-1 expression in NPC associated with tissue origin and the functional effects of STIM1 expression in NPC. Screening for target genes was using the KEGG PATHWAY database. The target gene analysis was initially done further studied using an immunohistostaining approach on NPC tissue samples. From the bioinformatic resources, STIM-1 has shown significant interaction with molecules network associated with cell migration and metastasis. Differentiated NPC showed moderate STIM-1 IHC staining intensity and undifferentiated NPC presented with strong STIM-1 IHC staining intensity. The present preliminary study suggests that STIM-1 could have a positive correlation with NPC pathogenesis. However, further comprehensive work using larger samples size is needed especially focusing on the STIM-1 expression and other clinicopathological parameters.

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Pathology classification of nasopharyngeal carcinoma

Cited by 2 publications

References 17 publications

Circ_0000285 regulates nasopharyngeal carcinoma progression through miR-1278/FNDC3B axis

Circ_0000285 regulates nasopharyngeal carcinoma progression through miR-1278/FNDC3B axis

Preliminary Analysis of Stim-1 Expression on Formalin-Fixed Paraffin-Embedded Nasopharyngeal Cancer Tissues

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