Pathological study of neoplasms surgically excised from animals attended the veterinary teaching hospital

Al-Ajeli, Radhwan R.; Al-Qadhi, Ahmed S.; Al-Mahmood, Saevan S.; Alkattan, Layth M.

doi:10.33899/ijvs.2019.126188.1260

Cited by 6 publications

(9 citation statements)

References 15 publications

(26 reference statements)

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“…Lesions detected by histopathological examinations, percentage of incidences were estimated, the 0 and 1 grading system were used to express lesion occurrence in each synovial capsule sample (19), also to express occurrence of foot-rot lesion or not. Data were analyzed using SPSS version 19 to determine the correlation coefficient values by 2-tailed Pearson correlation under significance at P≤0.01 (23).…”

Section: Discussionmentioning

confidence: 99%

“…Samples were trimmed, dehydrated, Cleared, impregnated and blocked in paraffin wax, then sectioned to 5 micrometer sections and stained with hematoxylin and eosin, dehydrated and mounted with DPX (18)(19)(20)(21)(22). The histopathological examination were done under light microscope with photographing using AMSCOPE digital camera.…”

Section: Histological Preparations and Examinationmentioning

confidence: 99%

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Pathological study of the relation between the incidences of claws lesions and carpo-digital joint capsule lesions in front limbs of sheep slaughtered in Mosul

Al-Mallah¹

2021

IJVS

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For detecting a possible relation between claws lesions and the lesions of carpo-digital joint capsule, the nearest joint to the claws, with an importance in diagnosing the exact cause of lameness in sheep resulting to be brought for casualty slaughter. For that goal 128 front limb were collected from slaughtered sheep at Mosul abattoir for period from 5-25/2/2013, the gross examination was made for observing the claws lesions and the synovial capsule were harvested, preserved and prepared for histopathologic examination, then examined microscopically with recording lesions. the correlation coefficient values were estimated between claws affection and joint capsular lesions at the same limb. Results demonstrated affection of 83 limbs with claws lesions as foot rot and erosions at incidence ratio 64.84%. The histopathological examination revealed a chronic type of changes including Fibroplasia in both synovium and tunica densa at incidences 8.59% and 6.25% respectively, Foci of hyperplastic synovial lining at 4.68% of samples, adipose tissue marbleizing synovium at 7.03% of the examined sections. Mild degree of chronic synovitis was observed within 10.93% of total samples, connective tissue metaplasia was evident at 3.9% of sections at tunica densa layer, the arteriolar musculature showed vacuolation at 18.75% of samples. The correlation coefficient value was significantly positive between occurrence of foot rot disease and the incidence of chronic synovitis in carpo-digital joint capsule which can be considered as the most important conclusion in the study.

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Section: Discussionmentioning

confidence: 99%

Section: Histological Preparations and Examinationmentioning

confidence: 99%

Pathological study of the relation between the incidences of claws lesions and carpo-digital joint capsule lesions in front limbs of sheep slaughtered in Mosul

Al-Mallah¹

2021

IJVS

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“…At every 2, 30 and 60 days of quails age and experimental days, 25 quails from each group were euthanized. Blood was collected to obtained serum tissue samples from the brain, intestines, lungs, testis, ovary, liver, and kidney were fixed in 10% formalin (9), samples from liver and kidney were collected and freeze at -20 ºC then homogenized using manual homogenizer to estimated MDA using manual photometric method by spectrophotometer at 450 nm wave length (10). Also, serum level of DEHP and MEHP were estimated using indirect ELISA test (11).…”

Section: Experimental Criteriamentioning

confidence: 99%

“…The samples of brain, intestines, lungs, testis, ovary, liver, and kidney were collected from both groups and fixed in 10% neutral buffered formalin for 72 hours, late a represented sample were having been chosen from these organs and dehydrated with ethyl alcohol, cleared by xylene, infiltrated and embedded by paraffin wax at 58 ºC (9). The tissue embedded in paraffin were section at 6 µm and stained with Harris' hematoxylin and alcoholic eosin (12).…”

Section: Histopathologymentioning

confidence: 99%

Effect DEHP plasticizer on MDA concentration and histopathological changes in Contronix contronix japonica

Hamoodi¹,

Al-Mahmood²

2021

IJVS

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In this experiment, we aimed to recorded the effect of chronic oral exposure to DEHP on lipid peroxidation and histopathological lesions induced in different organs. A total of 150 quail chick divided into two groups (75 quail's chicks at each group), control group left without any treatment all time, the second group was orally administrated 5% of DEHP (278.05 mg/kg bw) three times per week and lasted for 60 days. At 30 and 60 days old, a total number of 25 from both groups were euthanized. Blood samples were collected to determine the serum concentration of DEHP and mono ethyl hexyl phthalate (MEHP), liver and kidney to estimate tissue concentration of malondialdehyde (MDA), samples from different organs for histopathological examination. The result showed that the concentration of DEHP and MEHP in blood's serum and concentration of MDA in liver and kidney significantly increased with time in comparison with the control group; besides, oral administration of DEHP induced sever pathological lesions, in brain, aorta, intestines, liver, lung, ovary and testis. In conclusion, the DEHP and its metabolic product MEHP significantly increased in serum and cause significant increase in concertation of MDA in liver and kidney tissues, produces a significant histopathological change in brain, aorta, liver, intestine, lung, ovary and testis that can cause a life-threatening condition.

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“…During the 19 th -century, molecular pathology immunohistochemistry and electron microscopy were created and widely applied on scientific bases; these new techniques and protocols helped scientists to study diseases at the cellular and molecular levels (5)(6)(7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Standard techniques for formalin-fixed paraffin-embedded tissue: A Pathologist’s perspective

Al-Sabaawy¹,

Rahawi²,

Al-Mahmood³

2021

IJVS

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Histopathology considered as an essential keystone to understanding diseases on a cellular level, without examining affected tissues and cells; will lack the accurate diagnosis. The most common protocol that used worldwide for histopathology is termed as formalinfixed paraffin-embedded techniques; this protocol is the oldest protocol and still used, this technique not invented once, but it is started with a simple process of tissue fixation then undergo several successful steps as it's known today. This protocol depends on formalin to induce tissue fixation and preservation simultaneously, followed by a series of steps to facilitate infiltration of hot paraffin wax in the cells to adjust the density of tissue similar to that of paraffin wax to support tissue during sectioning process at few micrometers of thickness. Many protocols have been invented and modified subsequently to increase the accuracy of tissue processing and facilitate their study under light microscopy. Additionally, the formalin-fixed and paraffin-embedded samples can be considered the most durable process till now. In this review, we are trying to view all fundamental aspects of classical tissue processing that was depended since 1850s, starting from tissue examination, sampling, accessory, labelling, fixation, dehydration, clearance, infiltration, and embedding with hot paraffin wax. Additionally, explain the routine sectioning and staining with classical Hari's hematoxylin and alcoholic eosin staining protocol.

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Pathological study of neoplasms surgically excised from animals attended the veterinary teaching hospital

Cited by 6 publications

References 15 publications

Pathological study of the relation between the incidences of claws lesions and carpo-digital joint capsule lesions in front limbs of sheep slaughtered in Mosul

Pathological study of the relation between the incidences of claws lesions and carpo-digital joint capsule lesions in front limbs of sheep slaughtered in Mosul

Effect DEHP plasticizer on MDA concentration and histopathological changes in Contronix contronix japonica

Standard techniques for formalin-fixed paraffin-embedded tissue: A Pathologist’s perspective

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