Pathological Study of Naturally Occuring Ovine Pulmonary Adenocarcinoma in Sheep

Khodakaram-Tafti, Azizollah; Razavi, Zahra

doi:10.1016/j.jcpa.2009.08.066

Cited by 4 publications

(7 citation statements)

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“…The gross lesions observed in the lungs of adult sheep and microscopic proliferative changes observed in the alveolar and bronchiolar epithelium were consistent with the observations of the previous OPA studies [ 2 , 9 , 22 ]. There was infiltration of numerous macrophages in the lumen and in the vicinity of proliferated alveoli as previously reported by various workers in both natural and experimentally induced OPA [ 23 , 24 ].…”

Section: Discussionsupporting

confidence: 91%

Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep

Sonawane¹,

Tripathi²,

Kumar³

et al. 2016

Vet World

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Aim:This study was aimed to detect ovine pulmonary adenocarcinoma (OPA) in sheep flocks affected with pulmonary disorders at organized farm.Materials and Methods:A total of 75 sheep died naturally were thoroughly examined for the lesions of OPA during necropsy. Tissue sections from affected portion of the lungs from each animal were collected aseptically and divided into two parts; one each for polymerase chain reaction (PCR) and another for histopathology.Results:On PCR examination of lung tissues, six sheep (8%) were found to be positive for JSRV. Two of them were 3-6 months of age and did not show clinical signs/gross lesions of OPA. Four adult sheep positive on PCR revealed characteristic lesions of OPA on gross and histopathological examination.Conclusion:In the absence of known specific antibody response to the infection with JSRV, there is no diagnostic serological test available. The PCR assay employed in this study on lung tissues, using primers based on the U3 region of the viral long terminal repeat for JSRV would be helpful in the screening of preclinical and clinical cases of OPA in sheep.

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Section: Discussionsupporting

confidence: 91%

Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep

Sonawane¹,

Tripathi²,

Kumar³

et al. 2016

Vet World

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“…OPA was first reported in India during 1960 [1] and later on it was reported from different parts of the country [2]- [4]. The clinical symptoms, gross and histopathological lesions observed in the OPA affected sheep were similar to the earlier reports [4] [19]- [23]. The affected animals were anorectic and showed polypnea, dyspnea, cough and often pyrexia in the terminal stages of the disease and they were dead within 3 weeks [1].…”

Section: Discussionsupporting

confidence: 79%

“…Grossly, diffuse consolidated areas or discrete nodules seen in the affected lungs with moist surfaces were similar to that of classical form of OPA [1] [19] [23]- [25]. There are descriptions of classical and atypical forms of OPA in the literature [23]- [25]. Three anatomical forms consisting of grey nodules with moist surface in classical form, white, dry and firm nodules clearly demarcated, either solitary or multiple in atypical form and both characteristics in mixed form were described in lungs for the first time by De Las Heras et al [24] Later, the classical form of OPA characterized by a firm, greyish to purple neoplastic mass often surrounded by satellite tumor nodules with the cut section of tumor mass invariably having a wet appearance and copious fluid filling the airways and the atypical form showing a solitary tumor nodule in the diaphragmatic lobe and several smaller nodules in the main lobes, with hard and pearly white and a dry cut surface were reported [25].…”

Section: Discussionmentioning

confidence: 96%

Nucleotide Sequencing and Phylogenetic Analysis Using PCR Amplicons of U3 Gene of Jaagsiekte Sheep Retrovirus (JSRV) Detected in Natural Cases of Ovine Pulmonary Adenocarcinoma in India

Devi¹,

Yadav²,

Rao³

et al. 2014

OJVM

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Ovine pulmonary adenocarcinoma (OPA) caused by an exogenous Jaagsiekte sheep retrovirus (JSRV) is prevalent in Indian sheep. In the present study, OPA was diagnosed in sheep by clinical signs, gross and histopathology and polymerase chain reaction (PCR). Proviral DNA of exogenous JSRV was detected in lung tumor tissues, mediastinal lymph nodes, blood and lung fluid samples from natural cases of OPA by using U3-hn PCR and the PCR amplicons were sequenced to analyze nucleotide divergence. In total, six isolates were sequenced that had 96%-100% homology with a UK strain (AF105220.1) but more divergent from a South African strain (M80216) with 88%-93% identity. The phylogenetic analysis revealed segregation of the six isolates into two clusters. In conclusion, this study is the first report on sequencing and phylogenetic analysis of JSRV in India and further studies are suggested to know the complete sequencing and genetic divergence of JSRV in Indian sheep.

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“…Twenty-seven of the 31 OPA-positive sheep found in the 2015 UK survey) were recorded as adult animals, with the remaining four sheep classed as “age not recorded” (although these were thought likely to be adult sheep also) [ 18 ]. The geographical variation in OPA prevalence was shown by surveys in different parts of Iran where Khodakaram-Tafti and Razavi (2010) and Kajouri and Karimi (2002) found prevalences of 0.22% and 3%, respectively [ 19 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter

et al. 2017

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BackgroundOvine pulmonary adenocarcinoma (OPA), caused by Jaagsiekte sheep retrovirus (JSRV), is characterised by the development of invariably fatal lung tumours primarily in adult sheep. High infection rates and disease prevalence can develop during initial infection of flocks, leading to on-farm economic losses and animal welfare issues in sheep with advanced disease. The disease has been reported in Ireland and is notifiable, but the presence of JSRV has never been confirmed using molecular methods in this country. Additionally, due to the difficulties in ante-mortem diagnosis (especially of latently-infected animals, or those in the very early stages of disease), accurate information regarding national prevalence and distribution is unavailable. This study aimed to confirm the presence of JSRV in Ireland and to obtain estimates regarding prevalence and distribution by means of an abattoir survey utilising gross examination, histopathology, JSRV-specific reverse transcriptase polymerase chain reaction (RT-PCR) and SU protein specific immunohistochemistry (IHC) to examine the lungs of adult sheep.ResultsLungs from 1911 adult sheep were examined macroscopically in the abattoir and 369 were removed for further testing due to the presence of gross lesions of any kind. All 369 were subject to histopathology and RT-PCR, and 46 to IHC. Thirty-one lungs (31/1911, 1.6%) were positive for JSRV by RT-PCR and/or IHC but only ten cases of OPA were confirmed (10/1911, 0.5%) Four lung tumours not associated with JSRV were also identified. JSRV-positive sheep tended to cluster within the same flocks, and JSRV-positive sheep were identified in the counties of Donegal, Kerry, Kilkenny, Offaly, Tipperary, Waterford and Wicklow.ConclusionsThe presence of JSRV has been confirmed in the Republic of Ireland for the first time using molecular methods (PCR) and IHC. In addition, an estimate of OPA prevalence in sheep at slaughter and information regarding distribution of JSRV infection has been obtained. The prevalence estimate appears similar to that of the United Kingdom (UK). Results also indicate that the virus has a diverse geographical distribution throughout Ireland. These data highlights the need for further research to establish national control and monitoring strategies.Electronic supplementary materialThe online version of this article (10.1186/s13620-017-0111-z) contains supplementary material, which is available to authorized users.

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Pathological Study of Naturally Occuring Ovine Pulmonary Adenocarcinoma in Sheep

Cited by 4 publications

References 0 publications

Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep

Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep

Nucleotide Sequencing and Phylogenetic Analysis Using PCR Amplicons of U3 Gene of Jaagsiekte Sheep Retrovirus (JSRV) Detected in Natural Cases of Ovine Pulmonary Adenocarcinoma in India

First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter

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