Pathological Findings of Highly Pathogenic Avian Influenza Virus A/Duck/Vietnam/12/2005 (H5N1) in Turkeys

Abstract

The aim of present study was to identify the clinical signs, gross and histopathological findings in turkeys experimentally infected with highly pathogenic avian influenza virus A/duck/Vietnam/12/2005 (H5N1). Specific pathogens free of white turkeys having 6 weeks old were inoculated with 0.1 mL of the virus with 10 EID. Death was event at 3 DPI. Gross lesions observed after necropsy were splenomegaly, 5 50 pulmonary edema, severe congestion in lungs, hyperemia in brain as well as cecal-tonsil and congestion in skeletal muscle. Histopathological finding were multi organ necrosis and/or inflammation. The most consistent and severely affected organs were spleen, lungs, brain, pancreas and cecal-tonsil. It would be worth to mention that the lungs and spleen were most affected organs, among the others grossly and histopathologically, However to our knowledge, this is the first description of histopathological finding of highly pathogenic avian influenza virus A/Duck/Vietnam/12/2005 (H5N1) in turkey.

An Experimental Study on Apoptosis in Lymphoid Tissues of SPF Chicken after Avian Influenza Serotypes H9N2 Infection

“…Perkins et al, (2003) traced influenza virus serotype H5N1 in spleen tissue and reported that in 2 or 5 days after inoculation, viral antigens were cloned in histiocytosis of the spleen and induced histiocytosis, endothelial cells, and myocytes of spleen arterial straight muscle death [10]. Mehranpour et al, (2007) studied the effects of the H5N1 virus in turkeys and showed that the main damages happened in brain, pancreas and tonsil cecum [5]. The results of the present study were consistent with Mangkol, Roberto, Perkins, and Mehranpour and the role of the H9N2 virus in damaging lymphoid tissue were explained.…”

“…There is proven evidence of intensification of pathogenic effect of a H9N2 strain of influenza virus A separated from chickens in China by co-infections caused by golden staphylococcus and Haemophilus paragallinarum [5]. Developing our knowledge about the ways that influenza viruses interact with the host cells at the cell surface and the mechanisms and the pathways to induce cell death in the host helps us to find more efficient ways to deal with the disease.…”

A genetically engineered H5 protein expressed in insect cells confers protection against different clades of H5N1 highly pathogenic avian influenza viruses in chickens

“…The challenge virus (GenBank accession number CY016883) was characterized (Beato et al, 2007) and belongs to clade 2.3.2 (WHO, 2016). The virus was also characterized by its phenotype in turkeys (Mehrabanpour et al, 2007) and ducks (Beato et al, 2007). The diluted challenge virus was not back-titrated.…”