Pathological and Microbiological Studies on Pneumonic Lungs from Danish Calves

Tegtmeier, Conny; Uttenthal, Åse; Friis, Ν. F.; Jensen, N. E.; Jensen, Henrik Elvang

doi:10.1046/j.1439-0450.1999.00301.x

Cited by 64 publications

(63 citation statements)

References 19 publications

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“…However, the calf may have contracted the H. somni infection after the lavage and the P. multocida infection. [29] described H. somni as a frequent finding in autopsied calves with respiratory disease in Denmark. H. somni has seldom been found in Finland, with only 10 reports in the last 10 years and only in connection with respiratory disease [17].…”

Section: Discussionmentioning

confidence: 99%

Viral and Bacterial Pathogens in Bovine Respiratory Disease in Finland

Hartel

Nikunen²,

Neuvonen³

et al. 2004

Acta Vet Scand

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Härtel H, Nikunen S, Neuvonen E, Tanskanen R, Kivelä S-L, Aho P, Soveri T, Saloniemi H: Viral and bacterial pathogens in bovine respiratory disease in Finland. Acta vet. scand. 45, 193-200. -Pathogens causing bovine respiratory tract disease in Finland were investigated. Eighteen cattle herds with bovine respiratory disease were included. Five diseased calves from each farm were chosen for closer examination and tracheobronchial lavage. Blood samples were taken from the calves at the time of the investigation and from 86 calves 3-4 weeks later. In addition, 6-10 blood samples from animals of different ages were collected from each herd, resulting in 169 samples. Serum samples were tested for antibodies to bovine parainfluenza virus-3 (PIV-3), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCV), bovine adenovirus-3 (BAV-3) and bovine adenovirus-7 (BAV-7). About one third of the samples were also tested for antibodies to bovine virus diarrhoea virus (BVDV) with negative results. Bacteria were cultured from lavage fluid and in vitro susceptibility to selected antimicrobials was tested. According to serological findings, PIV-3, BAV-7, BAV-3, BCV and BRSV are common pathogens in Finnish cattle with respiratory problems. A titre rise especially for BAV-7 and BAV-3, the dual growth of Mycoplasma dispar and Pasteurella multocida, were typical findings in diseased calves. Pasteurella sp. strains showed no resistance to tested antimicrobials. Mycoplasma bovis and Mannheimia haemolytica were not found. bovine respiratory disease; calf; pneumonia; bacteria; virus; mycoplasma; tracheobronchial lavage; seroconversion.

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Section: Discussionmentioning

confidence: 99%

Viral and Bacterial Pathogens in Bovine Respiratory Disease in Finland

Hartel

Nikunen²,

Neuvonen³

et al. 2004

Acta Vet Scand

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“…In this study the RespoCheck bacterial multiplex real-time PCR (abbreviated to RespoCheck PCR) was evaluated with commonly used culture methods as reference. This PCR has been developed for the detection of four bacterial pathogens commonly associated with BRD [9, 13], i.e. Pasteurella multocida , Mannheimia haemolytica , Histophilus somni and Trueperella pyogenes .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a multiplex real-time PCR for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid

et al. 2017

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Background Pasteurella multocida, Mannheimia haemolytica, Histophilus somni and Trueperella pyogenes are four bacterial agents commonly associated with bovine respiratory disease (BRD). In this study a bacterial multiplex real-time PCR (the RespoCheck PCR) was evaluated for the detection in bronchoalveolar lavage fluid (BALF) of these four bacterial agents.ResultsThe analytical sensitivity of the multiplex real-time PCR assay determined on purified DNA and on bacterial cells of the four target pathogens was one to ten fg DNA/assay and 4 × 10−1 to 2 × 100 CFU/assay. The analytical specificity of the test was, as evaluated on a collection of 118 bacterial isolates, 98.3% for M. haemolytica and 100% for the other three target bacteria. A set of 160 BALF samples of calves originating from ten different herds with health problems related to BRD was examined with bacteriological methods and with the RespoCheck PCR. Using bacteriological examination as the gold standard, the diagnostic sensitivities and specificities of the four bacterial agents were respectively between 0.72 and 1.00 and between 0.70 and 0.99. Kappa values for agreement between results of bacteriological examination and PCRs were low for H. somni (0.17), moderate for P. multocida (0.52) and M. haemolytica (0.57), and good for T. pyogenes (0.79). The low and moderate kappa values seemed to be related to limitations of the bacteriological examination, this was especially the case for H. somni.ConclusionIt was concluded that the RespoCheck PCR assay is a valuable diagnostic tool for the simultaneous detection of the four bacterial agents in BALF of calves.

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“…It has been frequently isolated from pneumonic [1,8,18] and healthy [16,17] calves. By itself, this bacterium does not usually cause serious disease, but it can be a significant pathogen if associated with infections with other bacteria [7], viruses [15], or Mycoplasma [14,17] when calves are stressed [5]. It has been isolated frequently from the nasal cavity or pneumonic lesions of calves with BRD [8,9,13].…”

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confidence: 99%

Experimental Infection of Calves with Pasteurella multocida Serovar A:3 Isolated in Japan

Ishiguro

Kitajima

Kubota

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Pasteurella multocida, serovar A: 3, selected by pathogenicity in mice from among 10 strains isolated from pneumonic lesions of calves, was adjusted to 10 7 , 10 8 and 10 10 colony-forming units (CFU), and inoculated intratracheally into four calves. All calves showed pyrexia and had lungs with congestion and hepatization. Inoculation with 10 10 CFU of bacteria produced respiratory symptoms and abscesses in lungs. This information will aid elucidation of the pathogenicity of P. multocida and the development of vaccines.

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Pathological and Microbiological Studies on Pneumonic Lungs from Danish Calves

Cited by 64 publications

References 19 publications

Viral and Bacterial Pathogens in Bovine Respiratory Disease in Finland

Viral and Bacterial Pathogens in Bovine Respiratory Disease in Finland

Evaluation of a multiplex real-time PCR for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid

Experimental Infection of Calves with Pasteurella multocida Serovar A:3 Isolated in Japan

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