Pathological and Microbiological Investigations of Pneumonic Pasteurellosis in Sheep

Abstract

pneumonia suspected lung tissues were examined histopathologically, immunohistochemically and microbiologically, in Sanliurfa province. After definition of the macroscopic localization of the consolidated areas in the lungs, tissue samples were taken and preserved in cold chain and 10% formalin for microbiological and pathological examinations, respectively. For bacteriological examination of Pasteurella spp. lung specimens were inoculated in 7% sheep blood agar and Mc Conkey agar. After routine pathological tissue follow up procedure, tissues were embedded in paraffin and obtained sections stained with Hematoxylin&Eosin (H&E). The cases, having histopathological findings consistent with pneumonia, were forwarded to immunohistochemical (IHC) examinations to know whether lesions related to Mannheimia haemolytica and Pasteurella multocida using hyperimmune polyclonal rabbit sera in Avidin Biotin Complex Peroxidase (ABC-P). Microbiological, histopathological and immunohistochemical findings were comparatively evaluated in examined animals. Pasteurella multocida as a cause of pneumonia were detected in 38 cases of microbiological inoculations. Immunohistochemical staining resulted Mannheimia haemolytica (n=35) and Pasteurella multocida (n=30) positive. Immunohistochemically both Mannheimia haemolytica and Pasteurella multocida were positive in 23 cases and 45 animals were negative for both bacteria. The aim of this study is to show importance and role of Pasteurella spp, in sheep pneumonia in Sanliurfa region.

Detection of bovine respiratory syncytial virus, Pasteurella multocida, and Mannheimia haemolytica by immunohistochemical method in naturally-infected cattle

“…The diagnosis of Pasteurella spp. infection by bacteriological methods has been reported to be difficult due to many factors such as antibiotic treatment and frozen or autolytic material (28, 37). Especially when the tissues are contaminated with other microorganisms, a selective medium is required for the isolation of M. haemolytica and P. multocida .…”

“…Similar results were reported by Ozyildiz et al . (28). Detection of M. haemolytica antigens in pneumonic lungs by IHC is more reliable than detection by bacterial isolation.…”

“…Immunohistochemistry (IHC) is a safe technique and has been frequently used for diagnostic evaluations of Pasteurella spp. (24, 25, 28, 37) and BRSV (14) antigens in formalin-fixed tissues. Experimental studies have investigated the synergistic effect of the antigens in cattle by the IHC method (4, 27, 36).…”

Histopathological and immunohistochemical approaches for the diagnosis of Pasteurellosis in swine population of Punjab

“…These findings can be related to the findings of Ozyildiz et al . [ 20 ]. Mild positive reaction was also seen in lymphocytes in lymph nodes ( Figure-15 ).…”

Pneumonia in Algerian Ouled Djellal sheep: Bacteriological study and macroscopic aspect of lung lesions

“…Previous or combined infection with some respiratory viruses may increase the susceptibility of farm animals to secondary bacterial pneumonia (Cutlip et al, 1993;Ozyildiz et al, 2013). Sheep are particularly sensitive to parainfluenza virus type 3 (PIV-3) and bovine respiratory syncytial virus (BRSV); antibodies against these viruses have been demonstrated in this species (Lemhkuhl et al, 1985;Giangaspero et al, 1997).…”