Pathological And Immunological Study On Infection With Escherichia Coli In ale BALB/c mice

Ali, Intisar H; Jabir, Majid S.; Al-Shmgani, Hanady S.; Sulaiman, Ghassan M.; Sadoon, Ali H.

doi:10.1088/1742-6596/1003/1/012009

Cited by 64 publications

(31 citation statements)

References 20 publications

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“…Then, the animals were sacrificed, and blood samples were collected from the tail vein of all mice to obtain blood film, eosinophils count, immunoglobulin E (IgE) levels and IFN-c level. For the histopathological study, the lungs were separated and washed with PBS, fixed with 10% formalin, embedded with paraffin, sectioned and stained with haematoxylin and eosin [31]. A light microscope was used to examine a lung tissue sections from the different groups.…”

Section: Anti-allergic Activity Of Silver Nanoparticles: In Vivo Modelmentioning

confidence: 99%

Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction

Jabir

Hussien

Sulaiman

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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Nanoparticles (NPs) have biological activities like antibacterial, antifungal, drug delivery, immunomodulation and antitumor activities. The aim of the current study was to investigate some of biomedical applications of silver NP synthesis using extracts from leaves of Eriobotrya japonica. Colour changes, UV-visible spectroscopy, SEM, zeta potential, dynamic light scattering, FTIR and XRD were used to confirm AgNPs formation. The UV-vis spectrum absorption band was observed at almost 430 nm. The SEM image shows quasi-spherical shape of AgNPs. The zeta potential demonstrated the negative surface charge of NPs. FTIR results showed the functional groups of AgNPs. Crystalline nature of AgNPs was confirmed by XRD pattern. MTT assay was used to study the anti-proliferative activity against MCF-7 and HeLa cells. Apoptosis was tested using a DNA-fragmentation test, and expression of P 53 . AgNPs inhibited the proliferation of MCF-7 and HeLa cells, and reduced inflammation. Treatment with AgNPs significantly decreased allergic disorder. AgNPs stimulated the phagocytosis process in BMDMs. The results suggested that AgNPs could be a promising therapy for future and preventing inflammation, reduce allergic disorders and prevent bacterial infection through the up-regulation of phagocytosis. Hence, future work such as developed and improved NPs as adjuvants, immune-modulating substances and nano-drug delivery system is needed.

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Section: Anti-allergic Activity Of Silver Nanoparticles: In Vivo Modelmentioning

confidence: 99%

Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction

Jabir

Hussien

Sulaiman

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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“…If we introduce native bacteria as high as 10 9 CFU to BALB/c mice it should induce 50% lethality. [25] However, although a high dose of bacteria (8 ⋅ 10 8 CFU) was used, no lethal cases were observed during our experiments. We suppose that such a high viability can be a result of the presence of the PE shells, which could smoother the immune response of mice by shielding the LPS surface from direct contact with the immune cells.…”

Section: Discussionmentioning

confidence: 72%

“…On a basis of safe concentrations known from literature that can be introduced to an organism, vasculature capacity and distribution of cells within the body determined as collected signals in our experiments, we can use at least 1000 times less bacteria to obtain similar results without extensive circulation of bacterial cells elsewhere in the body. [2,25] Although in our experiments we did not use different modifications of the PE capsules, from the data obtained from the literature we can predict that the approach shown here can be further improved by using methods of modifications of the PE shell. One important condition using bacterial therapy is making bacteria stealth to the immune system.…”

Section: Discussionmentioning

confidence: 86%

In vivo capture of bacterial cells by remote guiding

Rybkin

Pinyaev

Sindeeva

et al. 2021

Preprint

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Recently, it has been shown that several bacterial strains can be very efficient in cancer treatment since they possess many important properties such as self-targeting, ease of detection, sensing and toxicity against tumors. However, there are only a few relevant “candidates” for such an approach, as targeting and detection one of the biggest challenges as well as there are many limitations in the use of genetic approaches. Here, it is proposed the solution that enables surface modification of alive bacterial cells without interfering with their genetic material and potentially reduces their toxic side effect. By the electrostatic interaction fluorescently labeled polyelectrolytes (PEs) and magnetite nanoparticles (NPs) were deposited on the bacterial cell surface to control the cell growth, distribution and detection of bacteria. According to the results obtained in vivo, by the magnet entrapment of the modified bacteria the local concentration of the cells was increased more than 5 times, keeping the high concentrations even when the magnet is removed. Since the PEs create a strong barrier, in vitro it was shown that the division time of the cells can be regulated for better immune presentation.

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“…JC-1 dye was employed for probing the membrane potential of the mitochondria. In brief, 24 h seeding of cells in 96well plates was followed by treatment with the IC 50 dose of the extracted active compound and staining with MMP dyes [26].…”

Section: Mitochondrial Membrane Potential Assaymentioning

confidence: 99%

TNF-α; Loaded on Gold Nanoparticles as a Good Therapeutic Agent against Breast Cancer AMJ13 Cells

Abood¹,

Jabir²,

Kadhim³

2020

Nano BioMed ENG

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Pathological And Immunological Study On Infection With Escherichia Coli In ale BALB/c mice

Cited by 64 publications

References 20 publications

Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction

Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction

In vivo capture of bacterial cells by remote guiding

TNF-α; Loaded on Gold Nanoparticles as a Good Therapeutic Agent against Breast Cancer AMJ13 Cells

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