Pathologic features and molecular identification of parelaphostrongylosis in a sitatunga (&lt;i&gt;Tragelaphus spekii&lt;/i&gt;)

Díaz‐Delgado, Josué; Cruz, David; Sobotyk, Caroline; Hensley, Terry; Anguiano, Maritza; Verocai, Guilherme G.; Gómez, Gabriel

doi:10.1292/jvms.21-0282

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Females also lack prominent horns. Wild populations of this ungulate have experienced a drastic decrease, hence they are mostly available in zoos or as exotic games in hunting ranches [4]. In fact, they were once believed to be extinct in Ghana until the specie was rediscovered by science in Avu Lagoon [5].…”

Section: Introductionmentioning

confidence: 99%

Morphological Studies on the Axial Skeleton of the Sitatunga (Tragelaphus spekii)

Onwuama

Ibe

Jaji

et al. 2022

Preprint

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Background: Anatomical features of the skeleton of wild animals contribute largely to their adaptation. A dearth of information on the skeletal anatomy of the sitatunga (Tragelaphus Spekii) necessitated this study. Two adult sitatunga carcasses weighing 54kg and 57kg were obtained after post-mortem examination. Bone preparation was achieved through cold water maceration protocol. Result: The tympanic bulla was elongated and massive, resulting in the rudimentary appearance of the styloid and muscular processes of the temporal bone. The lacrimal bone had a somewhat triangular presentation with the lacrimal foramen on the caudal border of the facial surface while its dorsal border formed the lateral margin of the frontal sinus. There was no observable lacrimal fossa on this facial surface of the lacrimal bone. The facial tubercle was absent. The vertebral column formula was C7 T13 L6 S4 C10-14, the atlas dorsal median tubercle was smooth, devoid of ridges. The thoracic spinous processes were oriented dorso-caudally from T1 to T11; spinous process of T12 was vertical, while that of T13 was oriented dorso-cranially. The length of the transverse process of L1 and L6 were the same, and smaller than the length of those of L2-L5. There was incomplete fusion of sacral spinous processes. Three dorsal and ventral sacral foramina were identified laterally on each side of the vertebrae. The ribs were 26 in number (13 pairs). The average number of bones of the axial skeleton was 75. Conclusion: This study recorded anatomical features of axial skeletal bones of the Sitatunga (Tragelaphus spekii) thereby providing a baseline data for future biomedical, archaeological and comparative skeletal anatomical studies.

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Section: Introductionmentioning

confidence: 99%

Morphological Studies on the Axial Skeleton of the Sitatunga (Tragelaphus spekii)

Onwuama

Ibe

Jaji

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Wild populations of this ungulate have experienced a drastic decrease, hence they are mostly available in zoos or as exotic games in hunting ranches [ 6 ]. In fact, they were once believed to be extinct in Ghana until the species was rediscovered by science in Avu Lagoon [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological and morphometric studies on the axial skeleton of the sitatunga (Tragelaphus spekii gratus)

Onwuama¹,

Ibe²,

Jaji³

et al. 2022

BMC Zool

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Background Anatomical features of the skeleton of wild animals contribute largely to their adaptation. A dearth of information on the skeletal anatomy of the sitatunga (Tragelaphus spekii gratus) necessitated this study. Two adult sitatunga carcasses weighing 54 kg and 57 kg were obtained after post-mortem examination. Bone preparation was achieved through cold water maceration protocol. Result The tympanic bulla was elongated and massive, resulting in the rudimentary appearance of the styloid and muscular processes of the temporal bone. The lacrimal bone had a somewhat triangular presentation with the lacrimal foramen on the caudal border of the facial surface while its dorsal border formed the lateral margin of the frontal sinus. There was no observable lacrimal fossa on this facial surface of the lacrimal bone. The facial tubercle was absent. The vertebral column formula was C7 T13 L6 S4 C10-14, and the atlas dorsal median tubercle was smooth, devoid of ridges. The spinous process of the axis extended the entire arch length to hang little above the odontoid process. The thoracic spinous processes were oriented dorso-caudally from T1 to T11; spinous process of T12 was vertical, while that of T13 was oriented dorso-cranially. The length of the transverse process of L1 and L6 were the same, and smaller than the length of those of L2-L5. There was incomplete fusion of sacral spinous processes. Three dorsal and ventral sacral foramina were identified laterally on each side of the vertebrae. The ribs were 26 in number (13 pairs). The sternum was comprised of 5 sternabrae separated by intersternal cartilage. The average number of bones of the axial skeleton was 75. Morphometric in formation included the length of skull, mandible and ribs; body length of vertebrae and spinous process length and height of the vertebrae. Conclusion This study recorded anatomical features and biometric information on axial skeletal bones of the Sitatunga (Tragelaphus spekii gratus) thereby providing baseline data for future biomedical, archaeological and comparative skeletal anatomical studies.

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Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection

Richards

Kania

Wilson

et al. 2023

Sci Rep

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This paper outlines methods used to identify novel antigens for use in the development of serological assays. Specifically, we applied these methods to a neurogenic parasitic nematode of cervids called Parelaphostrongylus tenuis. This parasite is of particular concern in both wild and domestic ungulates as it causes significant neurological signs and definitive diagnosis is only possible post-mortem, necessitating the development of serologic assays for antemortem diagnosis. Proteins extracted from P. tenuis organisms were affinity isolated using antibodies enriched from seropositive moose (Alces alces). The proteins were analyzed using mass spectrometry and liquid chromatography to obtain amino acid sequences that were then cross-referenced to open reading frames predicted from an assembled transcriptome. An antigen of interest was assessed for immunogenic epitopes and subsequently synthesized into 10-mer synthetic overlapping peptides representing these regions. These synthetic peptides were then assessed for reactivity against positive and negative moose sera and demonstrated potential use as a serological assay in diagnostic laboratories. Known negative moose sera revealed significantly lower optical density when compared to the positive samples (p < 0.05). This method serves as a pipeline for the construction of diagnostic assays of pathogens in both human and veterinary medicine.

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Pathologic features and molecular identification of parelaphostrongylosis in a sitatunga (<i>Tragelaphus spekii</i>)

Cited by 3 publications

References 24 publications

Morphological Studies on the Axial Skeleton of the Sitatunga (Tragelaphus spekii)

Morphological Studies on the Axial Skeleton of the Sitatunga (Tragelaphus spekii)

Morphological and morphometric studies on the axial skeleton of the sitatunga (Tragelaphus spekii gratus)

Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection

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