Pathologic Evidence of Ehrlichiosis in Calves Inoculated with <i>Ehrlichia chaffeensis</i>

Santos, Jose R. C. delos; Oglesbee, Michael; Rikihisa, Yasuko; Stich, Roger W.

doi:10.1196/annals.1428.068

Cited by 3 publications

(2 citation statements)

References 11 publications

(18 reference statements)

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“…In the United States Amblyomma americanum ticks are the transmitting vectors of E. chaffeensis and the white-tailed deer ( Odocoileus virginianus ) is the main reservoir host (Ewing et al, 1995), while humans and dogs are incidental hosts. Bovines are susceptible to experimental infection with E. chaffeensis (Delos Santos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)

Guillemi

Orozco

Argibay

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Vector-borne pathogens are responsible for serious emerging diseases and have been widely described in wildlife. Ehrlichia chaffeensis causes the zoonotic “monocytic ehrlichiosis” in humans, is transmitted by the tick Amblyomma americanum and its reservoir host is the white-tailed deer (Odocoileus virginianus) in North America. Little is known about the native reservoir and the tick vectors involved in the transmission cycle in South America. We report here the detection of E. chaffeensis in a study on marsh deer (Blastocerus dichotomus) mortality in Argentina, in different time periods between 2007 and 2016. Four deer, from two distinct populations, were positive for E. chaffeensis through molecular methods. Additionally, the variable-length PCR target (VLPT) region of positive samples was genotyped. Our results provide the first evidence of E. chaffeensis in autochthonous Cervidae from Argentina, contributing to uncover the distribution of this tick-borne infection in South America.

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Section: Introductionmentioning

confidence: 99%

Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)

Guillemi

Orozco

Argibay

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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“…[ 4 – 10 ]. A number of such Ehrlichia that might be responsible for the serological cross-reactivity have been described in domestic ruminants, including E. ovina in a sheep from Turkey [ 4 ], E. chaffeensis in goats and cattle in the USA [ 11 , 12 ], the Panola Mountain Ehrlichia in goats in the USA [ 9 ], and Ehrlichia sp. BOV2010/ Ehrlichia sp.…”

Section: Introductionmentioning

confidence: 99%

Development of a generic Ehrlichia FRET-qPCR and investigation of ehrlichioses in domestic ruminants on five Caribbean islands

et al. 2015

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BackgroundThe Ehrlichia are obligate intracellular Gram-negative tick-borne bacteria that are important human and animal pathogens. There is a need for assays to rapidly and reliably detect and differentiate the five generally recognized species into groups in a single reaction: E. canis, E. chaffeensis, E. ewingii, E. muris and E. ruminantium.MethodsWe developed primers and probes against the 16S rRNA gene to enable us to reliably detect the five major Ehrlichia spp. in a single FRET-qPCR. We tested the Ehrlichia FRET-qPCR on reference strains and on DNA from the blood of domestic ruminants from five Caribbean islands. The Ehrlichia present were determined using melting point analysis and by sequencing the Ehrlichia FRET-qPCR products as well as those of a nested PCR against the citrate synthase gene (gltA).ResultsOur Ehrlichia FRET-qPCR was negative for the closely related Anaplasma marginale and A. phagocytophilum but gave positive reactions with reference strains of the most generally recognized species and with other less characterized Ehrlichia of domestic ruminants, mainly E. ovina, the Panola Mountain Ehrlichia, and Ehrlichia sp. BOV2010. Melting point analysis revealed 4 distinct groups: E. ruminantium (Tm ~55.8 °C); E. chaffeensis and E. ewingii (Tm ~57.7 °C); E. canis, E. muris, E. ovina and Ehrlichia sp. BOV 2010 (Tm ~62.0 °C); and the Panola Mountain Ehrlichia (Tm ~65.5 °C). The detection limit of the FRET-qPCR was ~ 5 gene copies in a reaction and the sequences of the FRET-qPCR products were as expected. With DNA from domestic ruminants from the Caribbean we found 12.2 % (134/1,101) positive: cattle (76/385; 19.7 %), sheep (45/340; 13.2 %) and goats (13/376; 3.5 %). Melting point analysis and sequencing of the FRET-qPCR and nested PCR gltA products showed the Ehrlichia we detected were E. canis or very closely related organisms.ConclusionsIn a single reaction, our Ehrlichia FRET-qPCR can detect the Ehrlichia spp. we studied and differentiate them into four groups. Domestic ruminants in the Caribbean are not uncommonly exposed to Ehrlichia, possibly E. canis or very closely related organisms.

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Natural History of Ehrlichia chaffeensis: Vertebrate hosts and tick vectors from the United States and evidence for endemic transmission in other countries

Yabsley

2010

Veterinary Parasitology

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Pathologic Evidence of Ehrlichiosis in Calves Inoculated with Ehrlichia chaffeensis

Cited by 3 publications

References 11 publications

Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)

Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)

Development of a generic Ehrlichia FRET-qPCR and investigation of ehrlichioses in domestic ruminants on five Caribbean islands

Natural History of Ehrlichia chaffeensis: Vertebrate hosts and tick vectors from the United States and evidence for endemic transmission in other countries

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