Pathologic Analysis of Photothermal and Photomechanical Effects of Laser–tissue Interactions

Thomsen, Sharon

doi:10.1111/j.1751-1097.1991.tb09897.x

Cited by 522 publications

(325 citation statements)

References 48 publications

(34 reference statements)

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“…At four hours postheating, the tissue in this region appears viable, and histologic evidence of edema, inflammation, and hyperemia in an otherwise intact system accounts for the increase in permeability and leakage space observed on MRI. However, additional tissue damage may develop with time, and the observed hyperemic and inflammatory responses may be precursors to latent, lethal effects (34,35). Longer survival studies are required to determine the progression of changes and the ultimate fate of tissue in this region.…”

Section: Discussionmentioning

confidence: 99%

Prediction of subtle thermal histopathological change using a novel analysis of Gd‐DTPA kinetics

Cheng

Purcell

Bilbao

et al. 2003

Magnetic Resonance Imaging

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Purpose: To investigate Gd-DTPA kinetics as predictors of histopathological changes following focused ultrasound (FUS) thermal ablation for improved planning and assessment. Materials and Methods:Twenty-nine FUS lesions were created in the thigh muscle of eight rabbits under MRguidance at 1.5 Tesla. Three rabbits were killed at four hours; and 11 lesions were analyzed with histopathology. Temperature-sensitive MRI using proton-resonant frequency-shift was used for time-dependent temperature measurements. Analysis of the uptake kinetics of Gd-DTPA was performed after Gd-DTPA injection, within 20 minutes after heating and again at two hours after heating. The resulting kinetic maps, permeability (K trans ) and leakage space (v e ), were correlated to peak temperatures, T 2 -weighted MR, and histopathology.Results: Images of K trans and v e reveal regions of histopathological change not visible on conventional posttherapy MR. At early times after heating, v e predicts the area of injury more accurately than T 2 (7 Ϯ 2% vs. 25 Ϯ 6% underestimation). A circular region of extensive structural/ vascular disruption is indicated only on K trans maps. The sharp decrease in K trans at the boundary of this region occurs at 47.5 Ϯ 0.5°C, and may be a better estimate of cell death than the conventional method of temperature threshold (55°C for coagulation) used in therapy planning. Conclusion:Our results suggest Gd-DTPA kinetics can predict different histopathological changes following FUS ablation and may be valuable for early prediction.

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Section: Discussionmentioning

confidence: 99%

Prediction of subtle thermal histopathological change using a novel analysis of Gd‐DTPA kinetics

Cheng

Purcell

Bilbao

et al. 2003

Magnetic Resonance Imaging

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“…The effect of reactive hyperaemia on the size of thermal lesions in ARFI images is also unclear at this point. Reactive hyperaemia is a phenomenon not generally associated with in vitro tissues (Thomsen 1991, Liu et al 2004, and additional in vivo experiments are necessary to determine its impact on the accuracy of ARFI imaging.…”

Section: Discussionmentioning

confidence: 99%

Liver ablation guidance with acoustic radiation force impulse imaging: challenges and opportunities

et al. 2006

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Previous studies have established the feasibility of monitoring radiofrequency (RF) ablation procedures with acoustic radiation force impulse (ARFI) imaging. However, questions remained regarding the utility of the technique in clinically realistic scenarios and at scanning depths associated with abdominal imaging in adults. We address several of these issues and detail recent progress towards the clinical relevance of the ARFI technique. Results from in vitro bovine tissues and an in vivo ovine model are presented. Additional experiments were conducted with a tissue-mimicking phantom and parallel receive tracking techniques in order to further support the clinical feasibility of the method. Thermal lesions created during RF ablation are visualized with high contrast in both in vitro and in vivo hepatic tissues, and radial lesion growth can be monitored throughout the duration of the procedure. ARFI imaging is implemented on a diagnostic ultrasonic scanner, and thus may be a convenient option to guide RF ablation procedures, particularly when electrode insertion is also performed with sonographic guidance.

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“…Figure 3(b) shows solid lines marking the depth of collagen coagulation, while dashed lines show undamaged, structured collagen. Histological quantification of collagen coagulation [40] revealed a statistically significant correlation between probe contact time and burn severity as assessed by collagen coagulation at 24 hours post injury (Fig. 3(c)).…”

Section: Histology Measured Collagen Coagulation Changesmentioning

confidence: 99%

Quantitative assessment of graded burn wounds in a porcine model using spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI)

Ponticorvo¹,

Burmeister²,

Yang³

et al. 2014

Biomed. Opt. Express

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Accurate and timely assessment of burn wound severity is a critical component of wound management and has implications related to course of treatment. While most superficial burns and full thickness burns are easily diagnosed through visual inspection, burns that fall between these extremes are challenging to classify based on clinical appearance. Because of this, appropriate burn management may be delayed, increasing the risk of scarring and infection. Here we present an investigation that employs spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) as non-invasive technologies to characterize in-vivo burn severity. We used SFDI and LSI to investigate controlled burn wounds of graded severity in a Yorkshire pig model. Burn wounds were imaged starting at one hour after the initial injury and daily at approximately 24, 48 and 72 hours post burn. Biopsies were taken on each day in order to correlate the imaging data to the extent of burn damage as indicated via histological analysis. Changes in reduced scattering coefficient and blood flow could be used to categorize burn severity as soon as one hour after the burn injury. The results of this study suggest that SFDI and LSI information have the potential to provide useful metrics for quantifying the extent and severity of burn injuries. Durkin, "Spatial frequency domain imaging of burn wounds in a preclinical model of graded burn severity," J. ©2014 Optical Society of America

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Pathologic Analysis of Photothermal and Photomechanical Effects of Laser–tissue Interactions

Cited by 522 publications

References 48 publications

Prediction of subtle thermal histopathological change using a novel analysis of Gd‐DTPA kinetics

Prediction of subtle thermal histopathological change using a novel analysis of Gd‐DTPA kinetics

Liver ablation guidance with acoustic radiation force impulse imaging: challenges and opportunities

Quantitative assessment of graded burn wounds in a porcine model using spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI)

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