Pathognomonic features and ultrastructural of Koi Herpesvirus infected Oreochromis niloticus

Abstract

Abstract. Wahidi BR, Yanuhar U, Fadjar M, Andayani A. 2019. Pathognomonic features and ultrastructural of Koi Herpesvirus infected Oreochromis niloticus. Biodiversitas 20: 497-503. Koi Herpesvirus (KHV) has caused considerable losses in the cultivation of the Cyprinidae family worldwide. In addition to infecting Cyprinidae, KHV can also infect other freshwater fish. This study reports histopathology and ultrastructural analysis of KHV that infect Nile tilapia. Although there are no specific clinical symptoms, pathognomonic indicating Nile tilapia fish has been infected with KHV have been found, i.e. through the formation of intranuclear inclusion body and cell swelling which essentially experience chromatin margination. In addition, histopathology results indicate changes or damage to other tilapia tissue, i.e. necrosis, hypertrophy, edema, hemorrhage, inflammation, congestion, degeneration, and apoptosis. These results indicate that KHV has been distributed and replicated in tilapia organs. Based on the Transmission Electron Microscopy (TEM) analysis, KHV virions have been detected in the cytoplasm or outside the cells on a size of 150-170 nm.

Implementation of E-Government Policy from Governor Muhammad Zainul Madji (TGB) Era to Governor Zulkieflimansyah Era in West Nusa Tenggara Province

“…d. Encouraging various parties to increase their ability and willingness to develop, managing, and updating data and information content on an ongoing basis so that what is needed by related parties is available in real-time. Wahidi (2019) concluded that E-Government is a form of application for carrying out tasks and governance using telematics technology or information and communication technology. E-Government applications provide opportunities to improve and optimize relations between government agencies, the relationship between the government and the business world and society.…”

“…E-Government applications provide opportunities to improve and optimize relations between government agencies, the relationship between the government and the business world and society. The mechanism for this relationship is through information technology, a collaboration or merger between computers and communication network systems (Wahidi et al, 2019). E-Government Technology has caused much bureaucracy to be reorganized.…”

Electron microscopic observation of infected CCB and whole genome analysis of Koi herpesvirus isolate GY01

“…In an another report the morphology of KHV virus was simply divided into enveloped and non-enveloped virion (Dong et al, 2011). Ultrastructural analysis of gill tissues sampled from KHV infected Nile tilapia found that viral particles with a diameter of 150-170 nm in the cytoplasm or outside the gill cells (Wahidi et al, 2019) . Different size of capsids or virion particles in diameter in above researches probably due to the different cutting side of section or different stages of virus development but the morphologies were similar and consistent with viruses from the Herpesviridae family.…”