Pathogens isolated from clinical mastitis in Murrah buffaloes and their antibiogram

Charaya, Gaurav; Sharma, Anshu; Kumar, Ashok; Singh, Mahavir; Goel, Parveen

doi:10.14202/vetworld.2014.980-985

Cited by 16 publications

(15 citation statements)

References 5 publications

(7 reference statements)

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“…Taken together, antibiogram profile of bacteria associated with bubaline SCM suggests high resistance to β-lactam antibiotics, corroborating several other studies on bovine and bubaline mastitis in India [ 34 , 81 – 84 ]. The high antibiotic resistance could be due to indiscriminate and injudicious use of antibiotics, about which there is no authentic data available even for cattle in India.…”

Section: Resultssupporting

confidence: 88%

Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

et al. 2015

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Buffaloes are the second largest source of milk. Mastitis is a major impediment for milk production, but not much information is available about bubaline mastitis, especially subclinical mastitis. The aim of this study was to (a) investigate the application of various tests for the diagnosis of bubaline subclinical mastitis, (b) identify the major bacteria associated with it, and (c) evaluate the antibiotic resistance pattern of the bacteria. To this end, 190 quarter milk samples were collected from 57 domesticated dairy buffaloes from organized (64 samples) and unorganized (126 samples) sectors. Of these, 48.4%, 40.0%, 45.8%, 61.1%, and 61.6% were positive for subclinical mastitis by somatic cell count, electrical conductivity, California mastitis test, bromothymol blue test, and N-acetyl glucosaminidase test, respectively. As compared to the gold standard of somatic cell count, California mastitis test performed the best. However, a combination of the two methods was found to be the best option. Microbiological evaluation, both by biochemical methods as well as by monoplex and multiplex polymerase chain reaction, revealed that coagulase-negative staphylococci were the most predominant (64.8%) bacteria, followed by streptococci (18.1%), Escherichia coli (9.8%) and Staphylococcus aureus (7.3%). Most of the pathogens were resistant to multiple antibiotics, especially to β-lactam antibiotics. We propose that California mastitis test be combined with somatic cell count for diagnosis of subclinical mastitis in domestic dairy buffaloes. Further, our results reveal high resistance of the associated bacteria to the β-lactam class of antibiotics, and a possible major role of coagulase-negative staphylococci in causing the disease in India.

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Section: Resultssupporting

confidence: 88%

Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

et al. 2015

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“…Furthermore, a comprehensive examination of milk could offer important evidence concerning the nature of mastitis in dairy cattle and buffalo [ 35 ]. Bacterial species-wise prevalence estimates in this study were higher for S. aureus (38%) and St. dysgalactiae (15%), which were in agreement with a previous report [ 36 ]. Moreover, S. aureus in milk leads to food poisoning in human beings and is considered a public health concern.…”

Section: Discussionsupporting

confidence: 93%

An Understanding of the Global Status of Major Bacterial Pathogens of Milk Concerning Bovine Mastitis: A Systematic Review and Meta-Analysis (Scientometrics)

et al. 2021

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In this study, the major mastitis pathogen prevalence in the cattle and buffalo of the world was estimated by a meta-analysis. Staphylococcus (S) species, Streptococcus (St) species, and Escherichia coli (Ec) prevalence studies reported during 1979–2019 were collected using online databases, and offline resources. A meta-analysis of these data was done with the meta package in R-Software. The Staphylococcus aureus was the major mastitis pathogen, mostly causing subclinical mastitis, Ec causing clinical mastitis and St causing subclinical and clinical mastitis. The pooled prevalence estimates of S, St, and Ec were 28%, 12%, and 11% in the world from 156, 129, and 92 studies, respectively. The S, St, and Ec prevalences were high in Latin America (51%), Oceania (25%), and Oceania (28%), respectively. Higher S, St, and Ec prevalences were observed by molecular methods, signifying high sensitivity and usefulness for future studies. Among bacterial species, S. aureus (25%) followed by coagulase-negative Staphylococcus species (20%), Escherichia coli (11%), St. agalactiae (9%), St. uberis (9%) were the important pathogens present in the milk of the world. We hypothesize that there is a urgent need to reduce mastitis pathogen prevalence by ensuring scientific farm management practices, proper feeding, therapeutic interventions to augment profits in dairying, and improving animal and human health.

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“…The antibiogram profile of different bacterial isolates indicated that gentamycin, ciprofloxacin, enrofloxacin, tetracycline, Ampicilin and norfloxacin also proved as effective antimicrobials against S. aureus isolates in our study. Similar antibiogram patterns have been reported previously (Iqbal et al, 2004;Farooq et al, 2008;Charaya et al, 2014;Patnaik et al, 2014). This variability in sensitivity and resistance may be due to frequent and indiscriminate use of the antibiotics in different herds.…”

Section: Discussionsupporting

confidence: 89%

Molecular Characterization of Staphylococcus Aureus Isolates Recovered From Natural Cases of Subclinical Mastitis in Cholistani Cattle and Their Antibacterial Susceptibility

Qayyum¹,

Khan²,

Hussain³

et al. 2016

PAKJAS

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Subclinical mastitis primarily with microbial etiology is amenable to different antibiotic therapy and the outcome is doubtful due to the association of a variety of microorganisms, host-specificity and continual evolution of different antibiotic-resistant strains of microorganisms. Therefore, the present study was ascertained to investigate the presence, genotypic characteristics of Staphylococcus aureus isolates recovered from subclinically mastitic Cholistani cattle and the effectiveness of different antibiotics against these pathogens by disc diffusion technique. For this purpose milk samples were collected from a total of 1457 lactating Cholistani cattle and screened for mastitis using California Mastitis Test. All the positive samples were processed for culturing. Staphylococcus aureus isolates isolated and identified on the basis of colony characteristics, coagulase test, biochemical features and amplification of spa (spa-X) and coagulase (coa) genes. The results of PCR revealed that amplification of spa (spa-X) gene yielded different PCR products (400bp and 350bp) while coagulase (coa) produced different products size (390bp, 500bp, and 600bp) indicating genetic variation within and among different herds of the cattle. Moreover, results of this study showed that the spa (spa-X) gene present in coagulase positive (179) and coagulase negative (4) S. aureus isolates. S. aureus isolates were fully sensitive (88%) to amoxicillin, followed by enrofloxacin (78%) and highly resistant to penicillin (65%) and cephradine (100%). It is therefore concluded that S. aureus isolates were genetically different in the study areas and amoxicillin is the drug of choice for treating subclinical mastitis.

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Pathogens isolated from clinical mastitis in Murrah buffaloes and their antibiogram

Cited by 16 publications

References 5 publications

Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

An Understanding of the Global Status of Major Bacterial Pathogens of Milk Concerning Bovine Mastitis: A Systematic Review and Meta-Analysis (Scientometrics)

Molecular Characterization of Staphylococcus Aureus Isolates Recovered From Natural Cases of Subclinical Mastitis in Cholistani Cattle and Their Antibacterial Susceptibility

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