Pathogens in ticks collected in Israel: II. Bacteria and protozoa found in Rhipicephalus sanguineus sensu lato and Rhipicephalus turanicus

Mumcuoglu, Kosta Y.; Arslan-Akveran, Gonul; AYDOGDU, Sabiha; Karasartova, Djursun; KOŞAR, Ali; Savcı, Ünsal; Keskin, Adem; Taylan-Özkan, Ayşegül

doi:10.1016/j.ttbdis.2022.101986

Cited by 9 publications

(5 citation statements)

References 64 publications

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“…The B. vogeli sequence was identical to the reference sequence JF461252, which was previously detected in Romania [20]. This genetic variant was also found in Egypt [42] and Israel [43].…”

Section: Genbank Accession Nosupporting

confidence: 74%

Detection and Molecular Characterization of Canine Babesiosis Causative Agent Babesia canis in Naturally Infected Dogs in the Dobrogea Area (Southeastern Romania)

Ionita,

Leica,

Wassermann

et al. 2023

Life

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Canine babesiosis is an emerging tick-borne disease of major veterinary concern in Europe. Its prevalence has increased in the last two decades and is spreading rapidly toward the north. The aim of this study was to investigate the genetic diversity of Babesia spp. strains isolated from naturally infected dogs in a tick-endemic area (Dobrogea) in southeastern Romania. For this purpose, a total of twenty-three samples from dogs diagnosed with various clinical forms of babesiosis, evaluated by means of clinical history, physical examination, and hematological tests, were subjected to a molecular investigation using PCR, sequencing analysis, and genetic characterization. A microscopic examination of thin Diff-quick-stained blood smears revealed large intra-erythrocytic Babesia piroplasms in all dogs. The PCR and sequencing analysis results indicated the presence of Babesia canis in 22 dogs (95.7%) and Babesia vogeli in 1 dog (4.3%). Among the B. canis isolates, two genotypes were distinguished based on two nucleotide substitutions (GA→AG) observed in the 18S rRNA gene sequences (at positions 609 and 610), with the AG genotype predominating (54.5% of samples), while the GA variant was identified in 9.1% of samples. In the remaining isolates (36.4%), both variants were identified. The B. vogeli-positive dog also tested positive for antibodies against Ehrlichia canis and displayed severe disease. This study reports, for the first time, the presence of genetically heterogenic B. canis strains in dogs with clinical babesiosis in Romania. These findings provide a basis for future studies on the relationship between the genetic structure of the causative agents of canine babesiosis in Romania and the course of the disease.

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“…The B. vogeli sequence was identical to the reference sequence JF461252, which was previously detected in Romania [20]. This genetic variant was also found in Egypt [42] and Israel [43].…”

Section: Genbank Accession Nosupporting

confidence: 74%

Detection and Molecular Characterization of Canine Babesiosis Causative Agent Babesia canis in Naturally Infected Dogs in the Dobrogea Area (Southeastern Romania)

Ionita,

Leica,

Wassermann

et al. 2023

Life

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“…L. infantum DNA was also reported from questing Rhipicephalus spp. from Israel (Mumcuoglu et al 2022 ). Finally, further studies demonstrated the capability of R. sanguineus nourished on infected dogs to transmit the parasites to hamsters (Almeida et al 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Detection of Leishmania sp. kDNA in questing Ixodes ricinus (Acari, Ixodidae) from the Emilia-Romagna Region in northeastern Italy

et al. 2022

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To date, sand flies (Phlebotominae) are the only recognized biological vectors of Leishmania infantum, the causative agent of human visceral leishmaniasis, which is endemic in the Mediterranean basin and also widespread in Central and South America, the Middle East, and Central Asia. Dogs are the main domestic reservoir of zoonotic visceral leishmaniasis, and the role of secondary vectors such as ticks and fleas and particularly Rhipicephalus sanguineus (the brown dog tick) in transmitting L. infantum has been investigated. In the present paper, the presence of Leishmania DNA was investigated in questing Ixodes ricinus ticks collected from 4 rural areas included in three parks of the Emilia-Romagna Region (north-eastern Italy), where active foci of human visceral leishmaniasis have been identified. The analyses were performed on 236 DNA extracts from 7 females, 6 males, 72 nymph pools, and 151 larvae pools. Four samples (1.7%) (i.e., one larva pool, 2 nymph pools, and one adult male) tested positive for Leishmania kDNA. To the best of our knowledge, this is the first report of the presence of Leishmania kDNA in questing I. ricinus ticks collected from a rural environment. This finding in unfed larvae, nymphs, and adult male ticks supports the hypothesis that L. infantum can have both transstadial and transovarial passage in I. ricinus ticks. The potential role of I. ricinus ticks in the sylvatic cycle of leishmaniasis should be further investigated.

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“…Hedgehogs can be parasitised throughout their bodies, but especially around the eyes, ears, and anus. This species is also associated with the transmission of other pathogenic agents such as Rickettsia massiliae, R. conorii, and even Coxiella burnetti (the zoonotic agent responsible for Q fever) [53][54][55]. Among the species of the genus Ixodes spp., I. ricinus and Pholeoixodes hexagonus (previously known as Ixodes hexagonus) are two of the most prevalent species.…”

Section: Ticksmentioning

confidence: 99%

Hedgehogs’ Parasitology: An Updated Review on Diagnostic Methods and Treatment

Alfaia,

Jota Baptista,

Sós-Koroknai

et al. 2024

Parasitologia

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The genus Erinaceus is commonly found in rescue centres across the European continent despite the reported decline in some countries. Parasite infections are frequently detected in rescued hedgehogs, leading to increased morbidity and mortality and consequently conditioning their recovery. Some of the most frequent parasites include respiratory nematodes, such as Crenosoma striatum and Capillaria spp., which may lead to important pneumonia. Moreover, some of these agents have zoonotic potential, such as Cryptosporidium spp., Sarcoptes spp., and several species of ticks and fleas, which may transmit different vector-borne pathogens. This review provides a brief guide on hedgehogs’ internal and external parasitology, as well as some suggestions for diagnosis and treatment that are relevant for wildlife veterinarians, biologists and other researchers.

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Pathogens in ticks collected in Israel: II. Bacteria and protozoa found in Rhipicephalus sanguineus sensu lato and Rhipicephalus turanicus

Cited by 9 publications

References 64 publications

Detection and Molecular Characterization of Canine Babesiosis Causative Agent Babesia canis in Naturally Infected Dogs in the Dobrogea Area (Southeastern Romania)

Detection and Molecular Characterization of Canine Babesiosis Causative Agent Babesia canis in Naturally Infected Dogs in the Dobrogea Area (Southeastern Romania)

Detection of Leishmania sp. kDNA in questing Ixodes ricinus (Acari, Ixodidae) from the Emilia-Romagna Region in northeastern Italy

Hedgehogs’ Parasitology: An Updated Review on Diagnostic Methods and Treatment

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