Pathogenicity of the family Legionellaceae

Palusińska-Szysz, Marta; Cendrowska-Pinkosz, Monika

doi:10.1007/s00005-009-0035-8

Cited by 39 publications

(35 citation statements)

References 139 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Seven studies reported on the hazard of micro-organisms to HCWs and/or patients, see Table 5 study characteristics hazard in healthcare and the dental setting [12,31,34,45,[77][78][79] Three studies looked into the risks for patients when exposed to Legionella pneumophila containing sources that may produce bio-aerosols [34, 38,78]. They reported that cooling towers, air conditioning or mechanical ventilation systems could be a source of L. pneumophila.…”

Section: Hazard Of a Bio-aerosolmentioning

confidence: 99%

A scoping review on bio-aerosols in healthcare and the dental environment

et al. 2017

View full text Add to dashboard Cite

BackgroundBio-aerosols originate from different sources and their potentially pathogenic nature may form a hazard to healthcare workers and patients. So far no extensive review on existing evidence regarding bio-aerosols is available.ObjectivesThis study aimed to review evidence on bio-aerosols in healthcare and the dental setting. The objectives were 1) What are the sources that generate bio-aerosols?; 2) What is the microbial load and composition of bio-aerosols and how were they measured?; and 3) What is the hazard posed by pathogenic micro-organisms transported via the aerosol route of transmission?MethodsSystematic scoping review design. Searched in PubMed and EMBASE from inception to 09-03-2016. References were screened and selected based on abstract and full text according to eligibility criteria. Full text articles were assessed for inclusion and summarized. The results are presented in three separate objectives and summarized for an overview of evidence.ResultsThe search yielded 5,823 studies, of which 62 were included. Dental hand pieces were found to generate aerosols in the dental settings. Another 30 sources from human activities, interventions and daily cleaning performances in the hospital also generate aerosols. Fifty-five bacterial species, 45 fungi genera and ten viruses were identified in a hospital setting and 16 bacterial and 23 fungal species in the dental environment. Patients with certain risk factors had a higher chance to acquire Legionella in hospitals. Such infections can lead to irreversible septic shock and death. Only a few studies found that bio-aerosol generating procedures resulted in transmission of infectious diseases or allergic reactions.ConclusionBio-aerosols are generated via multiple sources such as different interventions, instruments and human activity. Bio-aerosols compositions reported are heterogeneous in their microbiological composition dependent on the setting and methodology. Legionella species were found to be a bio-aerosol dependent hazard to elderly and patients with respiratory complaints. But all aerosols can be can be hazardous to both patients and healthcare workers.

show abstract

Section: Hazard Of a Bio-aerosolmentioning

confidence: 99%

A scoping review on bio-aerosols in healthcare and the dental environment

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Legionnaire's disease is a severe form of pneumonia. Pontiac fever is the most common clinical manifestation and causes typical symptoms with characteristics of influenza infection, including fever, chills, and headache following a short incubation period [5,8]. Pontiac fever is the most common clinical manifestation and causes typical symptoms with characteristics of influenza infection, including fever, chills, and headache following a short incubation period [5,8].…”

mentioning

confidence: 99%

Inflammasome biology taught by Legionella pneumophila

Mascarenhas

Zamboni

2016

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Inflammasomes are multimeric protein complexes that assemble in the cytosol of many types of cells, including innate immune cells. The inflammasomes can be activated in response to infection or in response to stress signals that induce damage in the host cell membranes. These platforms trigger inflammatory processes, cell death, and the control of microbial replication. Many inflammasomes have been described so far, including NLRP3, NAIP/NLRC4, caspase-11, and AIM2. The ligand for NLRP3 is still unidentified, but the efflux of K is essential for NLRP3 activation. By contrast, inflammasomes, such as those composed of NAIP/NLRC4, caspase-11, and AIM2, can be activated by bacterial flagellin, LPS, and dsDNA. The knowledge of inflammasome biology has advanced tremendously in the last decade, fostered by the use of model organisms, such as This bacterium evolved, infecting unicellular protozoa in freshwater environments, and the human infection is accidental. Thus, did not evolve sophisticated mechanisms to inhibit mammalian innate immunity. For this reason, it has emerged as a very appropriate model of a pathogenic microbe for the investigation of inflammasome biology. In this review, we highlight the current information regarding the biology of inflammasomes and emphasize the advances achieved using We also describe the inflammasomes activated in response to infection and discuss the effector mechanisms that operate to clear the infection.

show abstract

“…This group contains with 84% of all infections the most important pathogenic Legionella [49]. Yet, since all Legionella species are regarded as potential pathogenic [40], a diagnostic test requires broad specificity to different Legionella serogroups and species. Thus, the specificity of a point of care test device needs to be extended to other subtypes, serogroups or species.…”

Section: Discussionmentioning

confidence: 99%

Human Anti-Lipopolysaccharid (LPS) antibodies against Legionella with high species specificity

Kühn

Thiem

Steinert

et al. 2019

HAB

View full text Add to dashboard Cite

Abstract. Legionella are Gram-negative bacteria that are ubiquitously present in natural and man-made water reservoirs. When humans inhale aerosolized water contaminated with Legionella, alveolar macrophages can be infected, which may lead to a lifethreatening pneumonia called Legionnaires' disease. Due to the universal distribution of Legionella in water and their potential threat to human health, the Legionella concentration in water for human use must be strictly monitored, which is difficult since the standard detection still relies on lengthy cultivation and analysis of bacterial morphology. In this study, an antibody against L. pneumophila has been generated from the naïve human HAL antibody libraries by phage-display for the first time. The panning was performed on whole bacterial cells in order to select antibodies that bind specifically to the cell surface of untreated Legionella. The bacterial cell wall component lipopolysaccharide (LPS) was identified as the target structure. Specific binding to the important pathogenic L. pneumophila strains Corby, Philadelphia-1 and Knoxville was observed, while no binding was detected to seven members of the families Enterobacteriaceae, Pseudomonadaceae or Clostridiaceae. Production of this antibody in the recombinant scFv-Fc format using either a murine or a human Fc part allowed the set-up of a sandwich-ELISA for detection of Legionella cells. The scFv-Fc construct proved to be very stable, even when stored for several weeks at elevated temperatures. A sensitivity limit of 4,000 cells was achieved. The scFv-Fc antibody pair was integrated on a biosensor, demonstrating the specific and fast detection of L. pneumophila on a portable device. With this system, 10,000 Legionella cells were detected within 35 min. Combined with a water filtration/concentration system, this antibody may be developed into a promising reagent for rapid on-site Legionella monitoring.

show abstract

Pathogenicity of the family Legionellaceae

Cited by 39 publications

References 139 publications

A scoping review on bio-aerosols in healthcare and the dental environment

A scoping review on bio-aerosols in healthcare and the dental environment

Inflammasome biology taught by Legionella pneumophila

Human Anti-Lipopolysaccharid (LPS) antibodies against Legionella with high species specificity

Contact Info

Product

Resources

About