Pathogenicity of PMV‐3/parakeet/netherlands/449/75 for chickens

Alexander, D. J.; Collins, Michael

doi:10.1080/03079458208436091

Cited by 24 publications

(22 citation statements)

References 7 publications

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“…In contrast to the well-studied APMV1 or NDV, very little is known about the biological characteristics, pathogenicity, and diversity (both genetic and antigenic) of other APMV serotypes 2-10. APMV types 2, 3, 6 and 7 have been associated with disease in domestic poultry [7-13]. APMV6 viruses have been associated with mild respiratory disease and decreased egg production in turkeys [14].…”

Section: Introductionmentioning

confidence: 99%

Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies

Rosseel

Lambrecht

Vandenbussche

et al. 2011

Virol J

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BackgroundDuring a wildlife screening program for avian influenza A viruses (AIV) and avian paramyxoviruses (APMV) in Belgium, we isolated two hemagglutinating agents from pools of cloacal swabs of wild mallards (Anas platyrhynchos) caught in a single sampling site at two different times. AIV and APMV1 were excluded using hemagglutination inhibition (HI) testing and specific real-time RT-PCR tests.MethodsTo refine the virological identification of APMV2-10 realized by HI subtyping tests and in lack of validated molecular tests for APMV2-10, random access amplification was used in combination with next generation sequencing for the sequence independent identification of the viruses and the determination of their genomes.ResultsThree different APMVs were identified. From one pooled sample, the complete genome sequence (15054 nucleotides) of an APMV4 was assembled from the random sequences. From the second pooled sample, the nearly complete genome sequence of an APMV6 (genome size of 16236 nucleotides) was determined, as well as a partial sequence for an APMV4. This APMV4 was closely related but not identical to the APMV4 isolated from the first sample. Although a cross-reactivity with other APMV subtypes did not allow formal identification, the HI subtyping revealed APMV4 and APMV6 in the respective pooled samples but failed to identify the co-infecting APMV4 in the APMV6 infected pool.ConclusionsThese data further contribute to the knowledge about the genetic diversity within the serotypes APMV4 and 6, and confirm the limited sensitivity of the HI subtyping test. Moreover, this study demonstrates the value of a random access nucleic acid amplification method in combination with massive parallel sequencing. Using only a moderate and economical sequencing effort, the characterization and full genome sequencing of APMVs can be obtained, including the identification of viruses in mixed infections.

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Section: Introductionmentioning

confidence: 99%

Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies

Rosseel

Lambrecht

Vandenbussche

et al. 2011

Virol J

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“…The poor recovery of virus from respiratory tract and brain may have resulted from the use of cell-free inocula because the regular recovery of NDV from different parts of the brain required co-cultivation (Wilczynski et al, 1977). Another PMV-3, 449 of psittacine origin, has been considered as a potential pathogen of chickens because it can reproduce experimental disease and stunting (Alexander and Collins, 1982) with lethality of one-day-old birds resulting from intramuscular but not intranasal or contact virus. However, MPHV was less pathogenic than 449 to chickens because no stunting or seroconversion followed contact exposure to virus and its mean ICPI of 0.41 was less than the value of 1.37 for 449 reported by Alexander and Chettle (1978) or 0.62 for 449 obtained here.…”

Section: Protection By Mabsmentioning

confidence: 99%

Pathogenicity of PMV‐3/Turkey/England/Midland poultry holdings/1981 for chickens and Turkeys

Russell

Awang

Parsons³

et al. 1989

Avian Pathology

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SUMMARYThe original 1981 English isolate of avian paramyxovirus type 3 from turkeys was of higher pathogenicity to chickens than the prototype 1968 isolate turkey/Wisconsin. It was lethal to chicks aged one day when given by intravenous or intracerebral inoculation. The age susceptibility was increased to one week by use of an inbred strain or betamethasone. The virus was more pathogenic to its natural host as outbred turkeys aged one week died after intravenous inoculation of the virus and contact transmission resulted in stunting and seroconversion of 2-week-old birds. Monoclonal antibodies to both viral glycoproteins, but not to the two internal proteins, protected one-day-old turkeys from 1000 intravenous LD50 of virus when they were inoculated one day after virus. This was considered to be analogous to maternally derived immunity and emphasised the role of yolk sac antibody in the protection of young stock.

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“…In another study, it was shown that the extent of disease and death in the birds after infection with APMV-3 strain Netherlands depends on dose as well as route of infection (Alexander and Collins, 1982b). The clinical signs of illness were more evident in 1-day-old birds compared to 2-weeks-old birds.…”

Section: Outcome Of the Studymentioning

confidence: 98%

“…These results confirmed previous findings that chickens and turkeys are susceptible to APMV-3 infection. Furthermore, younger birds are more susceptible than older birds (Alexander and Collins, 1982b;Russell et al, 1989). …”

Section: Outcome Of the Studymentioning

confidence: 99%

“…The virus causes acute pancreatitis and central nervous system (CNS) symptoms in Psittacine and Passerine birds (Beck et al, 2003). APMV-3 also infects chickens at an early age, with evidence of stunting growth that may be more marked in broiler chicken breeds (Alexander and Collins, 1982a). In terms of pathogenicity to domestic poultry birds, APMV-3 probably is second in importance to NDV.…”

Section: Introductionmentioning

confidence: 99%

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