Pathogenicity of Plectosphaerella species on lettuce and susceptibility of lettuce cultivars

Usami, Toshiyuki; Katagiri, Haruna

doi:10.1007/s10327-017-0736-5

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…Pathogenic Plectosphaerella spp. have been widely reported to cause fruit, root, and collar rot on several crops, becoming an emergent pathogen in recent decades (Carlucci et al 2012;Dillard et al 2005;Jimenez and Zitter 2005;Su et al 2017;Usami and Katagiri 2017;Vitale et al 2007). However, Plectosphaerella species can also exhibit other lifestyles in plants, as endophytes colonizing plant tissues without causing visible symptoms (D'Amico et al 2008;Götz et al 2006) or as epiphytes showing antagonistic effects against bacterial pathogens (Zhou et al 2017).…”

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confidence: 99%

Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains During Arabidopsis thaliana Colonization

Muñoz-Barrios

Sopeña-Torres

Ramos

et al. 2020

MPMI

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The fungal genus Plectosphaerella comprises species and strains with different lifestyles on plants, such as P. cucumerina, which has served as model for the characterization of Arabidopsis thaliana basal and non-host resistance to necrotrophic fungi. We have sequenced, annotated and compared the genomes and transcriptomes of three Plectosphaerella strains with different lifestyles on A. thaliana: PcBMM, a natural pathogen of wild-type plants (Col-0); Pc2127, a non-pathogenic strain on Col-0 but pathogenic on the immunocompromised cyp79B2 cyp79B3 mutant; and P0831 strain, that was isolated from a natural population of A. thaliana and is shown here to be non-pathogenic and to grow epiphytically on Col-0 and cyp79B2 cyp79B3 plants. The genomes of these Plectosphaerella strains are very similar and do not differ in the number of genes with pathogenesis-related functions, with the exception of secreted Carbohydrate-Active Enzymes (CAZymes) that are up to five times more abundant in the pathogenic strain PcBMM. Analysis of the fungal transcriptomes in inoculated Col-0 and cyp79B2 cyp79B3 plants at initial colonization stages confirm the key role of secreted CAZymes in the necrotrophic interaction, since PcBMM expresses more genes encoding secreted CAZymes than Pc2127 and P0831. We also show that P0831 epiphytic growth on A. thaliana involves the transcription of specific repertoires of fungal genes, that might be necessary for epiphytic growth adaptation. Overall these results suggest that in planta expression of specific sets of fungal genes at early stages of colonization determine the diverse lifestyles and pathogenicity of Plectosphaerella strains.

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confidence: 99%

Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains During Arabidopsis thaliana Colonization

Muñoz-Barrios

Sopeña-Torres

Ramos

et al. 2020

MPMI

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“…Plectosphaerella cucumerina AR1 is a filamentous Ascomycete fungus, mostly encountered in the terrestrial environment as a pathogen of various plant species and vegetables [e.g., lettuce (Usami and Katagiri, 2017), cabbage (Li et al, 2017), broomrape (Xu et al, 2016), sunflower (Zhang et al, 2015), bottle gourd (Yan et al, 2016), tomato, melon (Carlucci et al, 2012), potato (Gao et al, 2016)] and to a lesser extent in marine ecosystems where it has been described as host of ascidian invertebrates (López-Legentil et al, 2015), sponges (Wang et al, 2008) and shells (Velmurugan et al, 2011). To our knowledge, this is the first time that P. cucumerina (Figure 1) has been isolated from submerged plant litter in a freshwater ecosystem.…”

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confidence: 99%

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

et al. 2018

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Nicosulfuron is a selective herbicide belonging to the sulfonylurea family, commonly applied on maize crops. Its worldwide use results in widespread presence as a contaminant in surface streams and ground-waters. In this study, we isolated, for the first time, the Plectosphaerella cucumerina AR1 nicosulfuron-degrading fungal strain, a new record from Alnus leaf litter submerged in freshwater. The degradation of nicosulfuron by P. cucumerina AR1 was achieved by a co-metabolism process and followed a first-order model dissipation. Biodegradation kinetics analysis indicated that, in planktonic lifestyle, nicosulfuron degradation by this strain was glucose concentration dependent, with a maximum specific degradation rate of 1 g/L in glucose. When grown on natural substrata (leaf or wood) as the sole carbon sources, the Plectosphaerella cucumerina AR1 developed as a well-established biofilm in 10 days. After addition of nicosulfuron in the medium, the biofilms became thicker, with rising mycelium, after 10 days for leaves and 21 days for wood. Similar biofilm development was observed in the absence of herbicide. These fungal biofilms still conserve the nicosulfuron degradation capacity, using the same pathway as that observed with planktonic lifestyle as evidenced by LC-MS analyses. This pathway involved first the hydrolysis of the nicosulfuron sulfonylurea bridge, leading to the production of two major metabolites: 2-amino-4,6-dimethoxypyrimidine (ADMP) and 2-(aminosulfonyl)-N,N-dimethyl-3-pyridinecarboxamide (ASDM). One minor metabolite, identified as 2-(1-(4,6-dimethoxy-pyrimidin-2-yl)-ureido)-N,N-dimethyl-nicotinamide (N3), derived from the cleavage of the C-S bond of the sulfonylurea bridge and contraction by elimination of sulfur dioxide. A last metabolite (N4), detected in trace amount, was assigned to 2-(4,6-dimethoxy-pyrimidin-2-yl)-N,N-dimethyl-nicotinamide (N4), resulting from the hydrolysis of the N3 urea function. Although fungal growth was unaffected by nicosulfuron, its laccase activity was significantly impaired regardless of lifestyle. Leaf and wood surfaces being good substrata for biofilm development in rivers, P. cucumerina AR1 strain could thus have potential as an efficient candidate for the development of methods aiming to reduce contamination by nicosulfuron in aquatic environments.

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Ecophysiological characteristics of the nematophagous fungus, Plectosphaerella plurivora, with biocontrol potential on Nacobbus aberrans s.l. in tomato

et al. 2023

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Pathogenicity of Plectosphaerella species on lettuce and susceptibility of lettuce cultivars

Cited by 5 publications

References 15 publications

Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains During Arabidopsis thaliana Colonization

Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains During Arabidopsis thaliana Colonization

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

Ecophysiological characteristics of the nematophagous fungus, Plectosphaerella plurivora, with biocontrol potential on Nacobbus aberrans s.l. in tomato

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