Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice

Huang, Min; Wu, Yixin; Ho, Honhing; Li, Guozhi; He, Ping; GuoRu, Xiong; Yuan, Yuan; He, Yueqiu

doi:10.1016/s2095-3119(16)61334-5

Cited by 21 publications

(21 citation statements)

References 23 publications

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“…Klebsiella pneumoniae is also reported to be associated with plant root system, probably as nitrogen fixer in maize, poa and wheat, 13,14 but not yet reported as soil borne plant pathogen. Though Huang et al 10 reported Klebsiella pneumoniae causing bacterial top rot disease of maize; it was not described as soil borne plant pathogen. Klebsiella pneumoniae strain Borkar, is a soil borne plant pathogen infecting pomegranate plant by causing root bark necrosis and wilt in the plant.…”

Section: Discussionmentioning

confidence: 99%

“…In 2016 the bacterium Klebsiella pneumoniae was reported to infect maize plant in China causing bacterial top rot disease of maize in Yunnan province. 10 In subsequent year, its involvement as plant pathogen causing root bark necrosis and wilt in pomegranate was detected in pomegranate orchard of Mahatma Phule Agricultural University, Rahuri, India. 11 This opportunistic soil borne bacteria has emerged as a new soil borne plant pathogen particularly on pomegranate crop.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical characteristics of plant pathogenic Klebsiella pneumoniae causing root bark necrosis and wilt in pomegranate

Sg¹,

Ts²

2018

JABB

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A total 35 biochemical tests included in these identification test kit were used. These tests were ONPG, lysine utilization, ornithine utilization, urease, phenyl alanine deamination, Voges proskauer, AbstractA plant pathogenic Klebsiella pneumoniae causing root bark necrosis and wilt in pomegranate was designated as Klebsiella pneumoniae strain Borkar by NCBI (accession number: KY941097.1) on the basis of 16s rRNA partial gene sequence submitted. This strain was studied for its biochemical characteristics as it was a new bacterial pathogen causing plant disease and its biochemical characteristics was compared with the known biochemical characteristics of other Klebsiella pneumoniae sub species and Klebsiella species.Citation: Ajayasree TS, Borkar SG. Biochemical characteristics of plant pathogenic Klebsiella pneumoniae causing root bark necrosis and wilt in pomegranate.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biochemical characteristics of plant pathogenic Klebsiella pneumoniae causing root bark necrosis and wilt in pomegranate

Sg¹,

Ts²

2018

JABB

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“…are recognized mostly in the medical field as famous opportunistic germs associated with pathogenic infections [1,2]. K. pneumoniae represents a saprophytic pathogen that might affect both plants, animals, and humans [1,3,4], but it can be successfully used for biotechnological applications [5]. In medical terms, K. pneumoniae is a pathogenic strain responsible for multiple nosocomial infections, including pneumonia, urinary tract, and soft tissue infections, and septicemias [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Klebsiella pneumoniae—A Useful Pathogenic Strain for Biotechnological Purposes: Diols Biosynthesis under Controlled and Uncontrolled pH Levels

Mitrea

Vodnar

2019

Pathogens

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Despite being a well-known human pathogen, Klebsiella pneumoniae plays a significant role in the biotechnology field, being considered as a microbial cell factory in terms of valuable chemical biosynthesis. In this work, Klebsiella pneumoniae DSMZ 2026 was investigated for its potential to biosynthesize 1,3-propanediol (PDO) and 2,3-butanediol (BDO) during batch fermentation under controlled and uncontrolled pH levels. The bacterial strain was cultivated at a bioreactor level, and it was inoculated in 2 L of specific mineral broth containing 50 g/L of glycerol as the main carbon source. The process was conducted under anaerobic conditions at 37 • C and 180 RPM (rotations per minute) for 24 h. The effect of pH oscillation on the biosynthesis of PDO and BDO was investigated. Samples were taken every 3 h and specific tests were performed: pH measurement, main substrate consumption, PDO and BDO production. The cell morphology was analyzed on both solid and liquid media. After 24 h of cultivation, the maximum concentrations of PDO and BDO were 28.63 ± 2.20 g/L and 18.10 ± 1.10 g/L when the pH value was maintained at 7. Decreased concentrations of PDO and BDO were achieved (11.08 ± 0.14 g/L and 7.35 ± 0.00 g/L, respectively) when the pH level was not maintained at constant values. Moreover, it was identified the presence of other metabolites (lactic, citric, and succinic acids) in the cultivation media at the beginning of the process, after 12 h and 24 h of cultivation.

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“…are recognized mostly in the medical field as famous opportunistic germs associated with pathogenic infections [1][2][3]. Klebsiella pneumoniae represents a saprophytic pathogen that might affect both plants [4], animals [4,5] and humans [6][7][8], but which can be successfully used for biotechnological applications [9,10]. In medical terms, K. pneumoniae is a pathogenic strain responsible for multiple nosocomial infections including pneumonia, urinary tract and soft tissue infections, and septicemias [6,7,11].…”

Section: Introductionmentioning

confidence: 99%

Klebsiella pneumoniae – a useful pathogenic strain for biotechnological purposes: 1,3-propanediol biosynthesis

Mitrea

Trif

RUSU³

et al. 2019

Preprint

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Background Despite being a well-known human pathogen, Klebsiella pneumoniae plays a significant role in the biotechnology field, being considered as a microbial cell factory in terms of valuable chemicals biosynthesis. In fermentation processes, K. pneumoniae is mostly used to biosynthesize 1,3-propanediol (PDO), a very important intermediate compound utilized in the biodegradable bioplastics industry. This strain owns the ability to metabolize glycerol and to produce PDO under both aerobic and anaerobic circumstances, and under low oscillations of pH. Results In this work, we investigated the K. pneumoniae strain DSMZ 2026 in batch bioreactor cultivation. The bacterial strain was inoculated in 2 L culture broth containing 43.5 g·L- 1 main substrate and the pH was adjusted at 7 at the beginning of the process. After 12 h of batch cultivation, the pH values decreased to 4.77, and we obtained 51 g·L-1 wet biomass, 17 g·L-1 PDO, while the starting substrate reached 15 g·L- 1. The morphology of K. pneumoniae cells was analyzed on both solid and liquid media, by being identified large mucoid colonies on Columbia agar, and individual and grouped cells were observed by methyl blue staining. Conclusion From this study it can be concluded that K. pneumoniae can grow successfully in mineral broth under anaerobic environment and low decrease of pH, and can biosynthesize valuable chemicals like PDO.

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Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice

Cited by 21 publications

References 23 publications

Biochemical characteristics of plant pathogenic Klebsiella pneumoniae causing root bark necrosis and wilt in pomegranate

Biochemical characteristics of plant pathogenic Klebsiella pneumoniae causing root bark necrosis and wilt in pomegranate

Klebsiella pneumoniae—A Useful Pathogenic Strain for Biotechnological Purposes: Diols Biosynthesis under Controlled and Uncontrolled pH Levels

Klebsiella pneumoniae – a useful pathogenic strain for biotechnological purposes: 1,3-propanediol biosynthesis

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