PATHOGENICITY OF MYCOBACTERIUM INTRACELLULARE TO PIGS

Abstract

Summary The pathologic response of pigs to a strain of M. intracellulare serotype VI was investigated in three trials. Ten mg wet weight of the second subculture maintained on Löwenstein‐Jensen slopes produced a large number of tubercular nodules in the mesenteric lymph node of one pig infected orally, and similar lesions in the iliac lymph nodes of another pig following injection of the culture into the gluteal muscles. The lesions so produced were comparable with those recorded in the outbreak of tubercular lymphadenitis from which the above strain was isolated. In two other trials, variable doses ranging from 1.3 to 63 mg wet weight Löwenstein‐Jensen, harvested from the fourth and fifths subculture of this strain, were administered to 13 pigs. The number and size of lesions produced were much smaller, and in several instances no gross or microscopic lesions were detected. Further work is being done to investigate the factors resulting in decreased virulence of this strain of M. intracellulare for pigs.

Padronização do teste imunoalérgico aplicado ao diagnóstico da tuberculose e micobacterioses em suínos (Sus scrofa) experimentalmente sensibilizados com suspensões oleosas de Mycobacterium bovis ou M. avium inativados

“…Decorridos 60 dias do teste tuberculínico utilizado como triagem inicial, tempo esperado para que desaparecesse qualquer interferência do antígeno injetado (Tammemagi & Simmons 1971), os animais foram distribuídos em dois grupos com 25 indivíduos e outros dois grupos, um deles contendo 21 e o outro 20 animais, balanceando-se as características de raça, linhagem, faixa etária e sexo e inoculados via intramuscular na tábua do pescoço com os inóculos padrões e com os respectivos controles (dia experimental zero). O grupo A recebeu a injeção intramuscular de 0,5 mL de uma suspensão oleosa inativada de Myobacterium avium estirpe D4; o grupo B recebeu 0,5 mL de uma suspensão oleosa inativada de M. bovis estirpe AN5; o grupo C (controle I), recebeu 0,5 mL do adjuvante oleoso; e o grupo D (controle II) recebeu 0,5 mL de salina (solução fisiológica).…”

Estudo da transmissão horizontal de Mycobacterium avium em suínos

“…These are the attained results for each of the items: 1) there was a significant difference (p<0.05) between the mycobacterial recovery protocols from swine feces and the acid method with suspension in amphotericin solution and inoculation in Lowenstein-Jensen media with antibiotics presented the greatest recovery percentage (87%); 2) two fecal elimination periods of M. avium in swine feces were observed: an initial one, relative to the residual elimination of the inoculum, within days 1 and 4, and a second one, starting at day 18 and ending at day 62 post inoculation -the last one results from a supposed lesion opened to the intestinal lumen. (CLAAP, 1956;TAMMEMAGI;SIMMONS, 1971 na Austrália;THOEN et al, 1975 nos Estados Unidos;MEISSNER et al, 1978 na França e Alemanha;CASTRO et al, 1978;BALIAN et al, 1997 no Brasil;TAPARELLI;MARZADORI, 1990na Itália, SZABO et al, 1975 (BALIAN et al, 1997;CASTRO et al, 1978;EVERITT et al, 1982;PRITCHARD et al, 1977 (BALIAN et al, 1997;CORREA;CORREA, 1973;FERREIRA NETO et al, 1989;GONTIJO FILHO, 1974;LANGENEGGER et al, 1975;MOTA et al, 1980), M. flavescens e M.…”