Pathogenicity of Entomopathogenic Fungi From Lebanese Soils Against Aphids, Whitefly and Non-Target Beneficial Insects

Abstract

Three newly isolated and two existing isolates of M. anisopliae and B. bassiana were tested against aphids, whiteflies and beneficials, individually and with chitin synthesis inhibitor. Hundred percent of aphids have died of fungal treatments 7 days post inoculation, but number of individuals that developed mycosis varied depending on the isolate used. Whiteflies, however, were slightly more resistant. All tested isolates appeared to be fast killing fungi requiring only one or two days for reducing aphid population by half and needing 4 to 6 days for 50% of whiteflies to succumb to the disease. Both, aphid and whitefly, mortalities increased with increasing dose. The estimated LC50 values against M. persicae were 10 3.88 and 10 4.75 conidia ml -1 , respectively. These results were comparable with values obtained for the same isolates tested against whitefly Bemisia tabaci. Hundred precent of parasitized aphids by whitefly parasitoid demonstrated the emergence hole in the controls and in the individuals treated with new M. anisopliae isolate and 87% of emergence holes in the individuals treated with new B. bassiana. Exposure of mealybug predator to new isolates resulted in high mortalities. Native B. bassiana isolate in combination with diflubenzuron significantly reduced population of 1 st instar larvae of whiteflies under greenhouse conditions 14 days post treatment.

Efficacy of Some Predators and Lecanicillium lecanii Fungus in Controlling of Aphis gossypii (Glover) and Myzus persicae (Sulzer) in Potato Crop

“…In this study, the effects of metabolites and spore suspension of L. lecanii were investigated at different concentrations on some biological aspects of some developmental stages of the peach aphid (M. presicae), and the results obtained were similar to those obtained by Kim et al (2010) which revealed that degradation of insect body was observed in infiltrate treated aphids and the reduction of aphid population was more at high concentrations and high doses of filtrate treatments. Likewise, our results were in agreement with the results of Ibrahim et al (2011). In addition, our results were in agreement with those of Saranya et al (2010) who reported that L. lecanii, M. anisopliae and B. bassiana strains caused 100, 83.3 and 61.5% mortality, respectively, of cow pea aphid, Aphis craccivora after 7 days after treatment with a suspension of 10 7 spores/ml.…”

The Role of Chrysoperla carnea (Steph.) and Beauveria bassina for Controlling Cabbage Aphid, Brevicoryne brassicae L. on Cabbage Plants

“…El-Gendy (2015) found that the LC50 of biozed against B. brassicae was 55.8 ppm. Ibrahim et al (2011) found that the LC50 values were 103.88 and 104.75 conidia for the entomopathogenic fungi M. anisopliae and B. bassiana, respectively, against M. persicae. Moreover, Entesar et al (2020) reported that two isolates of B. bassiana (B1and B2) were evaluated against wheat aphid, Schizaphis graminum (Rondani), and the results showed that B1 isolate was the most effective.…”

Journal of Plant Protection Research

“…Two strains, B. bassiana and M. anisopliae, isolated from fallow soil (Ibrahim et al 2011) were used in this study. Long term storage of the isolates was achieved by freezing conidia in 35% w/w glycerol at −80°C.…”