Pathogenicity of conidia-based preparations of entomopathogenic fungi against the greenhouse pest aphids Myzus persicae, Aphis gossypii, and Aulacorthum solani (Hemiptera: Aphididae)

Jandricic, S.E.; Filotas, Melanie J.; Sanderson, John P.; Wraight, Stephen P.

doi:10.1016/j.jip.2014.02.003

Cited by 61 publications

(43 citation statements)

References 44 publications

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“…The identification of fungal isolates with proven efficacy against a broad range of aphid species should be a logical approach for the management of aphid on diverse crops, considering that most of them occupy the same ecological niche than strict specificity to one species. This assertion is in agreement with Jandricic et al (2014) who stated that the ideal mycoinsecticide for aphid management should be more consistent, with broader activity against a variety of Means within a column followed by the same lower case and within a row followed by the same upper case letters are not significantly different by Tukey's HSD multiple range test (P = 0. 05).…”

Section: Screenhouse Experimentssupporting

confidence: 89%

“…This suggests a reduction in population growth from both adults' mortality and reduction in fecundity following the application of conidial formulations as previously reported by Jandricic et al (2014). Among the two formulations, emulsifiable formulation performed better in terms of lower r i values on A. gossypii compared to the conidia formulated as aqueous, but there was no significant difference in terms of this parameter on B. brassicae and L. pseudobrassicae.…”

Section: Screenhouse Experimentssupporting

confidence: 80%

“…have been registered for aphid control. They include BotaniGard Ò and Naturalis-L Ò (B. bassiana-based products), Met52 Ò (M. brunneumbased product), Preferal Ò (Isaria spp-based product), and Vertalec Ò (Lecanicillium longisporum R. Zare & W. Gams-based product) (Jandricic et al 2014;Kabaluk et al 2010;Ravensberg 2011). Similar products are not available on the African continent.…”

Section: Introductionmentioning

confidence: 99%

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Selection of fungal isolates for virulence against three aphid pest species of crucifers and okra

et al. 2016

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Aphids are regarded as important pest problems of vegetable crops worldwide. Most vegetable growers in sub-Saharan Africa heavily rely on synthetic chemical insecticides for aphids' control. Fungus-based biopesticides are being considered as alternatives to chemical insecticides. This study evaluates virulence of five isolates of Metarhizium anisopliae and three of Beauveria bassiana against Brevicoryne brassicae, Lipaphis pseudobrassicae, and Aphis gossypii, and their thermotolerance and conidial yield as a prerequisite for strain selection. The study also evaluates performance of the best isolate in screenhouse experiment against target aphid species. Three isolates of M. anisopliae ICIPE30, ICIPE62, and ICIPE69 outperformed the others, causing mortality of 85-98 %, 83-97 %, and 73-77 %, in B. brassicae, L. pseudobrassicae, and A. gossypii, respectively, 7 days post-inoculation. Isolate ICIPE62 had the shortest LT 50 values of 3.4, 2.5, and 2.6 days at 1 9 10 8 conidia ml -1 , and the lowest LC 50 values of 7.3 9 10 5 , 9.3 9 10 4 , and 3.0 9 10 4 conidia ml -1 on day 7 against A. gossypii, B. brassicae, and L. pseudobrassicae, respectively. Furthermore, ICIPE62 produced more conidia on the surface of aphid cadavers than ICIPE30 and ICIPE69 and showed wider thermotolerance with optimum ranges between 25 and 30°C. Application of conidia of ICIPE62 formulated in aqueous and emulsifiable formulations negatively affected aphid population growth rate on kale and okra plants compared to controls in screenhouse experiments. These results have demonstrated the potential of M. anisopliae isolate ICIPE62 in suppression of A. gossypii, B. brassicae, and L. pseudobrassicae populations and could therefore be considered as biopesticide candidate for the control of these target aphids. Key messages• Several isolates of B. bassiana and M. anisopliae were screened for selection of isolate(s) that are virulent against three aphid pests infesting vegetables in subSaharan Africa. • M. anisopliae isolate ICIPE62 outperformed the other isolates in terms of virulence against the three aphid pests conidial production and thermotolerance. • Aqueous and emulsifiable formulations of M. anisopliae ICIPE62 provided acceptable control of the three aphid species in the screenhouse.

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Section: Screenhouse Experimentssupporting

confidence: 89%

Section: Screenhouse Experimentssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Selection of fungal isolates for virulence against three aphid pest species of crucifers and okra

et al. 2016

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“…Some of them have already been registered A c c e p t e d M a n u s c r i p t Bayissa et al 4 for aphid control in Europe and North America (de Faria & Wraight, 2007;Kabaluk, Svircev, Goettel, & Woo, 2010;Jandricic, Filotas, Sanderson, & Wraight, 2014).…”

Section: Introductionmentioning

confidence: 99%

Interactions among vegetable-infesting aphids, the fungal pathogenMetarhizium anisopliae(Ascomycota: Hypocreales) and the predatory coccinellidCheilomenes lunata(Coleoptera: Coccinellidae)

Bayissa

Ekesi

Mohamed

et al. 2015

Biocontrol Science and Technology

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Interactions among vegetable infesting aphids, the fungal pathogen Metarhizium anisopliae (Ascomycota: Hypocreales) and the predatory coccinellid Cheilomenes lunata (Coleoptera: Coccinellidae) AbstractEntomopathogenic fungi are among biocontrol agents being considered for the control of aphids on a variety of crops. Predatory coccinellids, although generalist, are also among important natural enemies that must be conserved for aphid management.Laboratory studies were carried out to investigate interaction between three vegetable infesting aphids, Metarhizium anisopliae isolate ICIPE 62 and the coccinellid predator Cheilomenes lunata. At concentration of 1  10 8 conidial ml -1 , the fungus was found to cause mortality of 7.5% to C. lunata, compared to 2.5% mortality in the control at 10 days post-treatment. Female adult C. lunata to which fungus-infected aphids were offered as prey never accepted them as food source in non-choice bioassays. However, live and dead non-infected aphids were fed upon. In choice bioassay, a total of 1-3 out of 24 infected non-sporulating aphids per species (average of 0.1-0.4 aphids per arena) were consumed by 48 h starved C. lunata within a period of 60 min, but avoided sporulating cadavers. Foraging adult C. lunata enhanced the spread of conidia of M.anisopliae from infected cadavers to fourth instars A. gossypii feeding on okra (0.8-15.0% mortality), B. brassicae (3.3-15.0% mortality) and L. pseudobrassicae (0.8-14.2% mortality) on kale plants. Results of this study demonstrate compatibility between M. anisopliae and C. lunata, and could provide a sustainable strategy for effective management of aphids on crucifers and okra cropping systems.

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“…Several studies have demonstrated the ability of the entomopathogenic fungi from the genus Metarhizium to suppress aphid populations [4,5,6]. Additionally, Metarhizium has been found to cause mycoses in an important soil dwelling pest in maize, Diabrotica virgifera (Col., Chrysomelidae) [7], and it shows persistence in maize fields [8].…”

Section: Introductionmentioning

confidence: 99%

Interactions among the Predatory Midge Aphidoletes aphidimyza (Diptera: Cecidomyiidae), the Fungal Pathogen Metarhizium brunneum (Ascomycota: Hypocreales), and Maize-Infesting Aphids in Greenhouse Mesocosms

Azevedo

Steinwender

Eilenberg

et al. 2017

Insects

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The generalist entomopathogenic fungus, Metarhizium brunneum, has proved to have great potential as a versatile biological pest control agent. The gall midge Aphidoletes aphidimyza is a specialist predator that occurs naturally in Europe and has been successfully used for aphid suppression. However, the interaction between these two biological control organisms and how it may affect the biological control of aphids awaits further investigation. As part of the EU-supported project INBIOSOIL, this study was conducted in greenhouse conditions to assess the possible effects of combining both biological control agents. In a randomized complete block design, sweet corn (Zea mays var. saccharata) plants were grown in large pots filled with natural soil or natural soil inoculated with M. brunneum. At the third leaf stage, before being individually caged, plants were infested with Rhopalosiphum padi and A. aphidimyza pupae were introduced in the soil. Aphidoletes aphidimyza midge emergence, number of living midges and number of aphids were recorded daily. The presence of conidia in the soil and on leaves was assessed during the experiment. At the conclusion of the experiment, the number of live aphids and their developmental stage, consumed aphids, and A. aphidimyza eggs was assessed under stereomicroscope. This study’s findings showed that the presence of M. brunneum did not affect A. aphidimyza midge emergence. However, longevity was significantly affected. As the study progressed, significantly fewer predatory midges were found in cages treated with M. brunneum compared to untreated cages. Furthermore, by the end of the study, the number of predatory midges found in the Metarhizium-treated cages was four times lower than in the untreated cages. Both daily and final count of aphids were significantly affected by treatment. Aphidoletes aphidimyza applied alone suppressed the aphid population more effectively than M. brunneum applied alone. Additionally, the aphid population was most suppressed when both agents were combined, though the suppression was less than additive.

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Pathogenicity of conidia-based preparations of entomopathogenic fungi against the greenhouse pest aphids Myzus persicae, Aphis gossypii, and Aulacorthum solani (Hemiptera: Aphididae)

Cited by 61 publications

References 44 publications

Selection of fungal isolates for virulence against three aphid pest species of crucifers and okra

Selection of fungal isolates for virulence against three aphid pest species of crucifers and okra

Interactions among vegetable-infesting aphids, the fungal pathogenMetarhizium anisopliae(Ascomycota: Hypocreales) and the predatory coccinellidCheilomenes lunata(Coleoptera: Coccinellidae)

Interactions among the Predatory Midge Aphidoletes aphidimyza (Diptera: Cecidomyiidae), the Fungal Pathogen Metarhizium brunneum (Ascomycota: Hypocreales), and Maize-Infesting Aphids in Greenhouse Mesocosms

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