Pathogenicity of animal and plant parasitic Aphanomyces spp and their economic impact on aquaculture and agriculture

Becking, Thomas; Kiselev, Andrei; Rossi, Valentina; Street-Jones, David; Grandjean, Frédéric; Gaulin, Elodie

doi:10.1016/j.fbr.2021.08.001

Cited by 18 publications

(14 citation statements)

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“…The term ‘root rot’ has been widely used to describe a group of diseases characterized by softening and necrosis of the roots, producing a broad spectrum of lesions of various colors and sizes ( Sharma et al., 2022 ). The widely spread oomycetes and fungi are the most prevalent soil-borne root rot pathogens ( Dean et al., 2012 ; Becking et al., 2021 ). Aphanomyces euteiches root rot (ARR) disease is one of the major limiting factors in the cultivation of North American ( Papavizas and Ayers, 1974 ; Wu et al., 2018 ) and European pea ( Quillévéré-Hamard et al., 2018 ; Quillévéré-Hamard et al., 2020 ), with some occurrence of this disease in Australian faba bean cultures ( Watson et al., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection

et al. 2023

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To successfully colonize the host, phytopathogens have developed a large repertoire of components to both combat the host plant defense mechanisms and to survive in adverse environmental conditions. Microbial proteases are predicted to be crucial components of these systems. In the present work, we aimed to identify active secreted proteases from the oomycete Aphanomyces euteiches, which causes root rot diseases on legumes. Genome mining and expression analysis highlighted an overrepresentation of microbial tandemly repeated proteases, which are upregulated during host infection. Activity Based Protein Profiling and mass spectrometry (ABPP-MS) on apoplastic fluids isolated from pea roots infected by the pathogen led to the identification of 35 active extracellular microbial proteases, which represents around 30% of the genes expressed encoding serine and cysteine proteases during infection. Notably, eight of the detected active secreted proteases carry an additional C-terminal domain. This study reveals novel active modular extracellular eukaryotic proteases as potential pathogenicity factors in Aphanomyces genus.

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Section: Introductionmentioning

confidence: 99%

The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection

et al. 2023

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“…The Aphanomyces genus belongs to the order Saprolegniales, which includes filamentous eukaryotic pathogens that are encountered in different terrestrial and aquatic ecosystems [1,2]. This genus includes about 45 species that infect plants, animals (fish, crustaceans) and agricultural crops [1,[3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…A. euteiches induces root rot, which causes seedlings' damping-off, yield decrease or even the death of the plant. This oomycete is highly virulent, mainly due to the production of a large number of motile biflagellate zoospores, which can move in liquid film in the soil and infect a whole field in a short time, leading to a complete loss of yield [2]. Several A. euteiches "pathotypes and/or races" have been identified based on their virulence on various legume hosts, such as pea or alfalfa [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

Kiselev

Clemente

Camborde

et al. 2022

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The soil-borne oomycete pathogen Aphanomyces euteiches causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of A. euteiches have been shown to exhibit differential quantitative virulence, but the molecular basis of host adaptation has not yet been clarified. Here, we re-sequenced a pea field reference strain of A. euteiches ATCC201684 with PacBio long-reads and took advantage of the technology to generate the mitochondrial genome. We identified that the secretome of A. euteiches is characterized by a large portfolio of secreted proteases and carbohydrate-active enzymes (CAZymes). We performed Illumina sequencing of four strains of A. euteiches with contrasted specificity to pea or alfalfa and found in different geographical areas. Comparative analysis showed that the core secretome is largely represented by CAZymes and proteases. The specific secretome is mainly composed of a large set of small, secreted proteins (SSP) without any predicted functional domain, suggesting that the legume preference of the pathogen is probably associated with unknown functions. This study forms the basis for further investigations into the mechanisms of interaction of A. euteiches with legumes.

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“…This is partly due to their superior competitive abilities (Lindqvist and Huner 1999;van Kuijk et al 2021), but also because these crayfish are major chronic carriers of the oomycete Aphanomyces astaci, a pathogen causing crayfish plague. More information on various aspects of this disease is provided in several recent reviews (Jussila et al 2015;Rezinciuc et al 2015;Svoboda et al 2017;Becking et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Long-term changes in the prevalence of the crayfish plague pathogen and its genotyping in invasive crayfish species in Czechia

Mojžišová

Svobodová

Kozubíková‐Balcarová

et al. 2022

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The widespread presence of North American alien crayfish in Europe is a major driver of native crayfish population declines, mainly because they are chronic carriers of the oomycete Aphanomyces astaci responsible for crayfish plague. Screening for the crayfish plague pathogen in host populations has become a common practice across Europe, but sampling usually covers spatial but not temporal variation. Our study focuses on the current situation in Czechia, where screening for A. astaci was first conducted in the mid-2000s. We provide data about the distribution and prevalence of this pathogen at almost 50 sites with three host crayfish: the spiny-cheek crayfish Faxonius limosus, signal crayfish Pacifastacus leniusculus, and marbled crayfish Procambarus virginalis. Among these sites were 20 localities that were resampled several years (usually more than a decade) after the original screening for A. astaci. We did not detect any A. astaci infection in two studied P. virginalis populations but documented several new hotspots of highly infected P. leniusculus in Czechia, and the first site with the coexistence of the latter with F. limosus. Our data suggest that despite some fluctuations, A. astaci prevalence in North American host populations generally does not tend to change significantly over time; we only observed two cases of a significant increase and one of a significant decrease. We no longer detected A. astaci in several originally weakly infected populations, but our data suggest it likely still persists in these areas and threatens native crayfish populations. At the single known site in the country where P. leniusculus and F. limosus coexist, we documented the presence of the same A. astaci genotype group in both crayfish species, likely due to interspecific transmission of the pathogen from the former host to the latter. However, genotyping of A. astaci in infected host individuals still supported the link between specific pathogen genotypes and crayfish hosts, suggesting that assessment of sources of mass mortalities from the pathogen genotyping is feasible in European regions where the mutual contact of different American crayfish species is uncommon.

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Pathogenicity of animal and plant parasitic Aphanomyces spp and their economic impact on aquaculture and agriculture

Cited by 18 publications

References 195 publications

The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection

The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

Long-term changes in the prevalence of the crayfish plague pathogen and its genotyping in invasive crayfish species in Czechia

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Pathogenicity of animal and plant parasitic Aphanomyces spp and their economic impact on aquaculture and agriculture

Cited by 18 publications

References 195 publications

The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection

The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infection

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

﻿Long-term changes in the prevalence of the crayfish plague pathogen and its genotyping in invasive crayfish species in Czechia

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Long-term changes in the prevalence of the crayfish plague pathogen and its genotyping in invasive crayfish species in Czechia