Pathogenicity Characterization of Prevalent-Type Streptococcus dysgalactiae subsp. equisimilis Strains

Matsue, Miki; Ogura, Kohei; Sugiyama, Hironori; Miyoshi‐Akiyama, Tohru; Takemori-Sakai, Yukiko; Iwata, Yasunori; Wada, Takashi; Okamoto, Shigefumi

doi:10.3389/fmicb.2020.00097

Cited by 9 publications

(9 citation statements)

References 55 publications

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“…This isolate was characterized by its extreme beta-hemolytic ability (data not shown). Matsue et al (Matsue et al, 2020) stated that predominant emm types of SDSE varied per country.…”

Section: Discussionmentioning

confidence: 99%

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Comprehensive comparative genomics analysis for the emerging human pathogen Streptococcus dysgalactiae subsp. equisimilis (SDSE): A case study and Pan-subspecies genomic analysis

Saeb

Tayeb

2021

Preprint

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Background: Streptococcus dysgalactiae subsp. equisimilis (SDSE) is the causal agent of various diseases that include wound infection, erysipelas, cellulitis, life-threatening necrotizing fasciitis, and streptococcal toxic shock syndrome. It is capable of infecting both humans and animals. In this investigation, we present a comprehensive genomic analysis for the SDSE strain SCDR1 that belongs to Lancefield group G, emm type (stG6) and (MLST) sequence type (ST44) that is the first time to be documented in Saudi Arabia and the middle east. Besides, we present the most comprehensive comparative genomics analysis for the emerging human pathogen SDSE. Methodology: We utilized next-generation sequencing techniques (NGS), bioinformatics, phylogenetic analysis, and comparative pathogenomics to characterize SCDR1 and all publicly available SDES genomes. Results: We found that SCDR1 consisted of a circular genome of 2179136 bp. Comparative analyses among bacterial genomes indicated that SCDR1 was most closely related to AC-2713 and GGS_124. Genome annotation of SCDR-1 strain showed that it contains many genes with homology to known virulence factors, including genes involved in cellular invasion, Antiphagocytosis, immune evasion, invasion of skin and soft tissue, host mortality and tissue damage, toxins, pore-forming proteins, cytotoxins, beta-hemolysis agents. Two CRISPR arrays were identified in SCDR1 that are consist of 35 CRISPR repeats and 33 CRISPR spacers. Two CAS systems were observed in the SCDR-1 genome, namely, CAS-TypeIIA and CAS-TypeIC. SDSE core Resistome is consisting of 22 genes, including folA, gyrA, gyrB, and FabK. SDSE core Virulome consisting of 38 genes including, fba, fbp54, gidA, and lsp. Conclusion: Our study confirmed that the SDSE strains possess different characteristics in producing virulence factors for pathogenicity to humans and based on its genome sequence and close relationship with GAS. Our study shed light on the proposed pathogenic mechanisms of SDSE and may form the basis of molecular epidemiological research on these highly virulent bacteria.

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“…This isolate was characterized by its extreme beta-hemolytic ability (data not shown). Matsue et al (Matsue et al, 2020) stated that predominant emm types of SDSE varied per country.…”

Section: Discussionmentioning

confidence: 99%

“…Streptococcus dysgalactiae subsp. equisimilis (SDSE) is an emerging human pathogen that causes severe invasive streptococcal diseases (Matsue et al, 2020). It belongs to β-hemolytic streptococci and possesses Lancefield group C or G antigens and rarely, A antigen (Takahashi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive comparative genomics analysis for the emerging human pathogen Streptococcus dysgalactiae subsp. equisimilis (SDSE): A case study and Pan-subspecies genomic analysis

Saeb

Tayeb

2021

Preprint

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“…HA concentration within the human knee joint is higher (2–3 mg/ml) than in any other fluid or tissue in the human body ( Balazs et al, 1967 ; Dahl et al, 1985 ). SDSE is often isolated from the skins and joint fluids ( Wajima et al, 2016 ; Matsue et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial strains SDSE-124 (GenBank accession number AP010935.1), SDSE-C167 (AP012976.1), GAS M1 476 (AP012491.2), GBS COH1 (HG939456.1), and GBS A909 (CP000114.1) were utilized in this study. Additional bacterial strains included TPCH-F36, which was isolated in Toyama Prefecture Central Hospital (Japan); KNZ01, KNZ03, KNZ10, and KNZ16 from Kanazawa University Hospital, Japan (Matsue et al, 2020); and GAS M1 SMD from National Center of Global Health and Medicine (Japan). The bacteria utilized in this study were cultured in Todd Hewitt broth (Becton Dickinson, Franklin Lakes, NJ, USA) supplemented with 0.2% yeast extract (THY, Becton Dickinson) or brain-heart infusion (BHI, Becton Dickinson); bacterial cultures were grown overnight at 37°C in a 5% CO 2 atmosphere.…”

Section: Bacterial Strainsmentioning

confidence: 99%

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Novel Hyaluronate Lyase Involved in Pathogenicity of Streptococcus dysgalactiae subsp. equisimilis

et al. 2020

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Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes cellulitis, bacteremia, and invasive diseases, such as streptococcal toxic shock syndrome. Although SDSE infection is more prevalent among elderly individuals and those with diabetes mellitus than infections with Streptococcus pyogenes (Group A streptococci; GAS) and Streptococcus agalactiae (Group B streptococci; GBS), the mechanisms underlying the pathogenicity of SDSE remain unknown. SDSE possesses a gene hylD encoding a hyaluronate lyase (HylD), whose homologue (HylB) is involved in pathogenicity of GBS, while the role of HylD has not been characterized. In this study, we focused on the enzyme HylD produced by SDSE; HylD cleaves hyaluronate (HA) and generates unsaturated disaccharides via a β-elimination reaction. Hyaluronate-agar plate assays revealed that SDSE promoted dramatic HA degradation. SDSE expresses both HylD and an unsaturated glucuronyl hydrolase (UGL) that catalyzes the degradation of HA-derived oligosaccharides; as such, SDSE was more effective at HA degradation than other β-hemolytic streptococci, including GAS and GBS. Although HylD shows some homology to HylB, a similar enzyme produced by GBS, HylD exhibited significantly higher enzymatic activity than HylB at pH 6.0, conditions that are detected in the skin of both elderly individuals and those with diabetes mellitus. We also detected upregulation of transcripts from hylD and ugl genes from SDSE wild-type collected from the mouse peritoneal cavity; upregulated expression of ugl was not observed in ΔhylD SDSE mutants. These results suggested that disaccharides produced by the actions of HylD are capable of triggering downstream pathways that catalyze their destruction. Furthermore, we determined that infection with SDSEΔhylD was significantly less lethal than infection with the parent strain. When mouse skin wounds were infected for 2 days, intensive infiltration of neutrophils was observed around the wound areas Nguyen et al.

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Comparative genome analysis of Streptococcus strains to identify virulent genes causing neonatal meningitis

Arya

Sharma

Kumar

et al. 2023

Infection, Genetics and Evolution

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Pathogenicity Characterization of Prevalent-Type Streptococcus dysgalactiae subsp. equisimilis Strains

Cited by 9 publications

References 55 publications

Comprehensive comparative genomics analysis for the emerging human pathogen Streptococcus dysgalactiae subsp. equisimilis (SDSE): A case study and Pan-subspecies genomic analysis

Comprehensive comparative genomics analysis for the emerging human pathogen Streptococcus dysgalactiae subsp. equisimilis (SDSE): A case study and Pan-subspecies genomic analysis

Novel Hyaluronate Lyase Involved in Pathogenicity of Streptococcus dysgalactiae subsp. equisimilis

Comparative genome analysis of Streptococcus strains to identify virulent genes causing neonatal meningitis

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