Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets

Kim, Hyun-Jeong; Park, Jun-Gyu; Alfajaro, Mia Madel; Kim, Deok-Song; Hosmillo, Myra; Son, Kyu-Yeol; Lee, Ju-Hwan; Bae, You-Chan; Park, Sang-Ik; Kang, Mun-Il; Cho, Kyoung-Oh

doi:10.1016/j.vetmic.2012.03.017

Cited by 14 publications

(32 citation statements)

References 49 publications

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“…The RVA in 3 out of 7 MLN cases was detected by dFAT (Fig.3b) and RT-PCR along with histopathological lesions like depleted lymphocytes from germinal centre (Fig.2d). The presence of RVA in extra-intestinal site has been reported in natural and experimental cases of mice, calf and pigs (Crawford et al, 2006;Kim et al, 2012). In the present study, 4 MLNs were found negative though the corresponding intestines and contents were positive for RVA both by RT-PCR and dFAT.…”

Section: Resultssupporting

confidence: 64%

Rotavirus A associated pathology of intestine and mesenteric lymph nodes and occurrence in bovine calves of Gwalior and Bareilly regions

Kashyap

Malik

Agrawal

et al. 2018

IJAR

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To understand the pathology of natural cases of rotavirus (RVA) in bovine calves, a total of 40 cases below 6 months died due to diarrhoea were studied, out of which 7 cases (17.5%) turned positive for RVA by RT-PCR. Histopathology of small intestine showed loss of villous enterocytes, blunting and fusion of villi, elongation of crypts and mononuclear cells infiltration in the lamina-propria. The mesenteric lymph nodes were severely depleted of lymphocytes. These changes were corroborated with presence of RVA antigen in sections by dFAT and nucleic acid by RT-PCR. The fluorescent signals were more in mesenteric lymph nodes than in intestine. Besides, 115 rectal fecal samples were also collected from calves for RVA detection by RT-PCR using VP6 gene specific sets of primers. Dead carcasses of calves (n= 40) belonged to organized dairy farm of Bareilly, while rectal fecal samples belonged to both organized (n= 38) and unorganized farms (n= 77) of Bareilly and Gwalior. The overall occurrence of RVA was 19.3% (30/155), comprising 5/37 cases (13.5%) from Gwalior (MP) and 25/118 cases (21.1%) from Bareilly (UP). These findings suggest the infection of RVA widely prevalent in calves and have potential to escape from the intestinal site to mesenteric lymph nodes.

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Section: Resultssupporting

confidence: 64%

Rotavirus A associated pathology of intestine and mesenteric lymph nodes and occurrence in bovine calves of Gwalior and Bareilly regions

Kashyap

Malik

Agrawal

et al. 2018

IJAR

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“…For example, porcine-like RVA G5P[7] strains were found in Korean cattle herds and vice versa, bovine-like RVA G6P[1] strains were sporadically detected in some Argentinean pig herds (Lorenzetti et al, 2011; Supplementary Table plus References 37,52). Interestingly, reassortant bovine-porcine RVAs with advantageous genetic configurations have been demonstrated to retain the ability to infect and cause disease in the heterologous host (Park et al, 2011;Kim et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Review of group A rotavirus strains reported in swine and cattle

Papp

Békési

Jakab

et al. 2013

Veterinary Microbiology

202

221

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. Group A rotavirus (RVA) infections cause severe economic losses in intensively reared livestock animals, particularly in herds of swine and cattle. RVA strains are antigenically heterogeneous, and are classified in multiple G and P types defined by the two outer capsid proteins, VP7 and VP4, respectively. This study summarizes published literature on the genetic and antigenic diversity of porcine and bovine RVA strains published over the last 3 decades. The single most prevalent genotype combination among porcine RVA strains was G5P [7], whereas the predominant genotype combination among bovine RVA strains was G6P [5], although spatiotemporal differences in RVA strain distribution were observed. These data provide important baseline data on epidemiologically important RVA strains in swine and cattle and may guide the development of more effective vaccines for veterinary use. ß

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“…Waterborne transmission of RVAs could serve as an alternative explanation for the occurrence of RVA with mixed genotype constellations as water resources may be polluted by fecal specimens of multiple host species. Little is known about the respiratory route of RVA transmission under natural conditions, although airborne transmission of rotavirus has been proposed by early epidemiological findings and later demonstrated by animal experiments [112][113][114]. The question, however, whether infective rotavirus particles are shed through respiratory secretions is open and awaits further experimental data.…”

Section: Evidence and Mechanisms Of Rotavirus Zoonosismentioning

confidence: 99%

Zoonotic transmission of rotavirus: surveillance and control

Dóró

Farkas

Martella

et al. 2015

Expert Review of Anti-infective Therapy

101

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Group A rotavirus (Rotavirus A, RVA) is the main cause of acute dehydrating diarrhea in humans and numerous animal species. RVA shows vast diversity and a variety of human strains share genetic and antigenic features with animal origin RVA strains. This finding suggests that interspecies transmission is an important mechanism of rotavirus evolution and contributes to the diversity of human RVA strains. RVA is responsible for half a million deaths and several million hospitalizations worldwide. Globally, two rotavirus vaccines are available for routine use in infants. These vaccines show a great efficacy profile and induce protective immunity against various rotavirus strains. However, little is known about the long-term evolution and epidemiology of RVA strains under selective pressure related to vaccine use. Continuous strain surveillance in the post-vaccine licensure era is needed to help better understand mechanisms that may affect vaccine effectiveness.

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Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets

Cited by 14 publications

References 49 publications

Rotavirus A associated pathology of intestine and mesenteric lymph nodes and occurrence in bovine calves of Gwalior and Bareilly regions

Rotavirus A associated pathology of intestine and mesenteric lymph nodes and occurrence in bovine calves of Gwalior and Bareilly regions

Review of group A rotavirus strains reported in swine and cattle

Zoonotic transmission of rotavirus: surveillance and control

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