Pathogenicity bioassays of isolates of<i>Beauveria bassiana</i>on<i>Rhynchophorus ferrugineus</i>

Nineteen different isolates of the entomopathogenic fungi Beauveria bassiana sensu lato (s.l.) and Metarhizium anisopliae s.l. (Ascomycota: Hypocreales), recovered from different soil samples (field crops, fruit orchards, vegetable fields and forests) and insect cadavers were tested against Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) at two different spore concentrations (1 × 107 and 1 × 108 conidia mL−1). Three isolates of B. bassiana and two of M. anisopliae gave >88 % larval and >75 % adult mortality of R. ferrugineus on their highest dose rate respectively. More sporulating cadavers (mycosis) resulted from a high dose rate compared to low dose on both life stages of R. ferrugineus. The current study confirmed the lethal action of B. bassiana and M. anisopliae isolates with mortality levels usually directly proportional to the conidial concentration. This study further confirmed that the isolates recovered from R. ferrugineus dead cadavers gave more mortality compared to the other sources. In the virulence assay two isolates of B. bassiana caused the highest percentage of both larval and adult mortality at all exposure intervals which suggest that they may be the most promising for use in sustainable management programs aimed at microbial control in date palm orchards.

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“…Lo Verde et al . () also reported the significantly higher pathogenic action of B. bassiana strains isolated from RPW. El‐Sufty et al .…”

Section: Discussionmentioning

confidence: 83%

Virulence of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against red palm weevil, Rhynchophorus ferrugineus (Olivier)

Yasin

Wakil

Ghazanfar

et al. 2017

Entomological Research

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“…Frequently, studies to select entomopathogenic fungi for the biological control of pest insects are based on the analysis of mortality and virulence of available isolates (Barta 2010;Lo Verde et al 2015). However, the analysis of the biological aspects of these microorganisms, such as radial growth and sporulation, provides more information on the physiology and behavior of the isolates, expanding the characteristics evaluated for the selection of fungi with potential in the largescale utilization for the biological control of pests.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Isolates of Entomopathogenic Fungi in the Genera Metarhizium,Beauveria, and Isaria, and Their Virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

Santos

Freitas

Poderoso

et al. 2018

Florida Entomologist

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“…and Metarhizium anisopliae (Metchn.) Sorokin (Hypocreales: Clavicipitaceae) have been isolated from wild R. ferrugineus populations (Lo Verde et al, 2015). Beaveria bassiana fungus could be used as one of the biological strategies in controlling red palm weevil (Hajjar et.…”

Section: Resultsmentioning

confidence: 99%

Isolation, phenotypic and genotypic characterization of indigenous Beauveria bassiana isolates from date palm infested with Rhynchophorus ferrugineus in Hail region, Saudi Arabia

Asiry¹,

Sulieman²,

Alanazi³

et al. 2018

Biosci. Biotech. Res. Comm

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The red palm weevil (RPW) Rhynchophorus ferrugineus overrun date palm ranches in many parts of Saudi Arabia, henceforth causes massive economic losses. Integrated pest Management (IPM) of the RPW by utilizing Entomopathogenic fungi (Beauveria bassiana), which has antagonistic activity against many insect pests, was the main objective of the current study. In the present study, soil samples, samples of dead red palm weevils (RPW) and palm fronds were collected according to RPW incidence map of Hail region, Saudi Arabia. Isolates of entomopathogenic fungi were isolated from the dead RPW adults and larvae. The fungal culture (BSA1, BH-2 and BH-3) was preserved and maintained for further analysis. Morphological and biochemical characterization of the antagonist fungi were employed and confi rmed that the fungus belonged to Beauveria spp. Further, this fungal isolates were propagated and prepared for genetic characterization. Sequencing of internal transcribed spacers ( ITS1 and ITS2) region was shown that three polymorphic ITS regions. The molecular identifi cation of the fungus strain was employed at King Faisal Universitythe College of Agriculture and Food Science and confi rmed that the fungus identifi ed as Beauveria bassiana as the fi rst record of this benefi cial species in Hail region.

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Pathogenicity bioassays of isolates ofBeauveria bassianaonRhynchophorus ferrugineus

Abstract: This study shows that the pathogenicity of wild isolates of B. bassiana differs among the tested R. ferrugineus instars. The low mortality of treated adults supports their use as vectors of B. bassiana as a potential tool for reducing R. ferrugineus populations.

Cited by 32 publications

References 16 publications

Virulence of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against red palm weevil, Rhynchophorus ferrugineus (Olivier)

Virulence of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against red palm weevil, Rhynchophorus ferrugineus (Olivier)

Evaluation of Isolates of Entomopathogenic Fungi in the Genera Metarhizium,Beauveria, and Isaria, and Their Virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

Isolation, phenotypic and genotypic characterization of indigenous Beauveria bassiana isolates from date palm infested with Rhynchophorus ferrugineus in Hail region, Saudi Arabia

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