Pathogenicity and population structure analysis of <i>Pyricularia oryzae</i> in different districts of Jiangsu province, China

Qi, Zhongqiang; X, Pan; Du, Yan; Shen, Lerong; Yu, Mina; Cao, Huijuan; Song, Tianqiao; Yu, Junjie; Zhang, Rongsheng; Yong, Mingli; Yin, Xiuli; Liu, Yongfeng

doi:10.1111/ppa.13283

Cited by 7 publications

(3 citation statements)

References 36 publications

(32 reference statements)

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“…According to the International Code of Nomenclature for algae, fungi and plant, the name of the anamorph (Pyricularia) should be used over the teleomorph (Magnaporthe) (Luo and Zhang 2013;Murata et al 2014;Klaubauf et al 2014), but both genus names continue to be used, depending on the research group (Perelló et al 2015;D'Ávila et al 2016Farman et al 2017;Yasuhara-Bell et al 2018b;Dorigan et al 2019;Asuke et al 2019;Tembo et al 2020;Qi et al 2021). A proposition of a new name for the species that cause wheat blast was recently made, based on phylogenetic and pathogenic data (Castroagudín et al 2016).…”

Section: Taxonomy and Genetic Diversitymentioning

confidence: 99%

“…For example, mixed infections in shared hosts favors gene flow through sexual recombination or non-meiotic parasexual processes, (Talbot et al 1993a, b;Soanes et al 2002;Inoue et al 2017;Monsur and Kusaba 2018;Zheng et al 2018;Gómez Luciano et al 2019;Asuke et al 2019), mutations in avirulence genes through selecting frequently occurs (Orbach et al 2000), or through the moving of a Pot3 transposon across pathogenicity related-genes (Kang et al 2001). Pathogenic effector proteins have been under evolutive selection across blast populations (Sweigard et al 1995;Farman and Leong 1998;Jones and Dangl 2006;Avila-Adame 2014;Tosa et al 2016;Wang and Valent 2017;Inoue et al 2017Inoue et al , 2021Gladieux et al 2018;Langner et al 2018;Gómez Luciano et al 2019), which might explain the ability to infect a new host or the variable levels of aggressiveness across different hosts (Kato et al 2000;Tosa et al 2004;Castroagudín et al 2016;Perelló et al 2017;Urashima et al 2017;Reges et al 2018Reges et al , 2019Qi et al 2021).…”

Section: Taxonomy and Genetic Diversitymentioning

confidence: 99%

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Taxonomic and pathogenic diversity of the blast pathogen populations infecting wheat and grasses in Minas Gerais

Ascari¹,

Ponte²

2021

Preprint

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The blast disease of Poaceae is caused by a large species complex, among which P. oryzae is composed of several host-specialized lineages. The Pyricularia oryzae Triticum pathotype (PoT) causes the blast disease in wheat, but is also capable of infecting other grasses, which may serve as an inoculum reservoir for epidemics in wheat. In Brazil, severe wheat blast epidemics are most common in the Cerrado region. The dominant hypothesis is that signal grass (Urochloa sp.) and other gramineous plants harbor the wheat blast pathogen, thus serving as a major reservoir of inoculum for epidemics in wheat. A two-year survey of the Pyricularia blast pathogens was conducted in both wheat and non-wheat areas as well as prior (February) and during (May) the wheat growing season in Minas Gerais. A total of 1,368 plant samples representative of 31 Poaceae species, including wheat, were collected and inspected for the presence of blast symptoms. During the isolations, 932 isolates were obtained, being one fourth obtained from gramineous plants. A subset of 572 isolates was selected for identification at the species level based on portions of the CH7-BAC9 gene sequences. Most of the isolates (n = 494) were P. oryzae, within which 68% were PoT and 32% non-PoT based on two PCR assays targeting (MoT3 and C17 PCR assays). The PoT lineage was found predominantly (97%) in wheat and rarely in the other hosts, even nearby wheat fields (2.1%), as well as at longer distances from wheat regions (0.1%). The blast pathogen population isolated from signal grass grouped in different clades from PoT, and therefore referred to Urochloa lineage (PoU). A series of cross-inoculation greenhouse experiments was conducted using wheat (cv. BRS Guamirim and BR 18-Terena) and signal grass (cv. Marandu) as host and 14 PoT and six PoU isolates as pathogen factor. In the first leaf-inoculation experiment, results showed a significant interaction between host and pathogen; PoT was strongly/weakly aggressive towards wheat/signal grass and PoU was strongly/weakly aggressive towards signal grass/wheat. In inoculated wheat heads, PoT was more aggressive (>91% infected spikelets) than PoU (52% infected spikelets). In a third experiment, four signal grass cultivars (Marandu, Basilisk, Piatã, and Xaraés) were inoculated with the same set of 20 isolates. Similarly, signal grass cultivars were generally more susceptible to PoU than PoT. Severity induced by PoU was twice (7.7% severity) as high as PoT (3.8%) and so was the number of conidia/leaf produced by PoU (47,500) and PoT (23,200). Two groups of signal grass cultivars were formed, the most susceptible composed of Marandu and Basilisk and the least susceptible composed of Piatã and Xaraés. Results of our study confirm the host-specialization and the shaping of the blast populations according to the host. We further suggest that grasses in general, especially signal grass, may not play a major role as an inoculum reservoir for PoT, as it harbors mainly the PoU population. However, due to the large extent of pasture-growing regions and cross-infection ability in wheat, signal grass may harbor amounts of PoT inoculum that are sufficient for initiating leaf and head blast epidemics in wheat blast in Minas Gerais state.

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Section: Taxonomy and Genetic Diversitymentioning

confidence: 99%

Section: Taxonomy and Genetic Diversitymentioning

confidence: 99%

Taxonomic and pathogenic diversity of the blast pathogen populations infecting wheat and grasses in Minas Gerais

Ascari¹,

Ponte²

2021

Preprint

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“…lines in 2022, that provided by Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences(Qi et al 2021). Method of spore culture and inoculation was performed as previously described(Wang et al 2016).…”

mentioning

confidence: 99%

A Genome-wide association study of panicle blast resistance (PBR) to Magnaporthe Oryzae in Rice

Jinlong,

Yu,

Ruizhi

et al. 2024

Preprint

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Rice blast, caused by Magnaporthe Oryzae (M. oryzae), is one of the most serious diseases all over the world. Development of blast-resistant rice varieties can effectively control the damage of rice blast and reduce the application of chemical pesticides. In this study, 477 sequenced rice germplasms from 48 countries were inoculated and identified at booting stage. We found that 23 germplasms displayed high panicle blast resistance against M. oryzae. 43 quantitative trait loci (QTLs) significantly (P < 10− 4) associated with rice panicle blast resistance were identified by genome-wide association analysis (GWAS). These QTL intervals contain four genes (OsAKT1, OsRACK1A, Bsr-k1 and Pi25/Pid3) have been reported to be involved in rice blast resistance. We select QTLs with -Log10(Pvalue) higher than 6.0 or those detected in two-year replicates, totaling 12 QTLs, for candidate gene analysis. We identified three blast resistance candidate genes (Os06g0316800, Os06g0320000, Pi25/Pid3) based on the significant SNP distribution of annotated gene sequences in these 12 QTL and the difference of expression levels among blast resistant varieties after 72h inoculation. Os06g0316800 encodes a protein similar to Glycine-rich protein, an important component of plant cell walls involved in cellular stress responses and signaling, named as OsGrp6. Os06g0320000 encodes a protein of unknown function DUF953, belonging to the thioredoxin-like family, crucial for maintaining reactive oxygen species (ROS) homeostasis in vivo, named as OsTrxl1. Lastly, Pi25/Pid3 encodes a disease resistance protein, emphasizing its potential significance in plant biology. By analyzing the haplotypes of these 3 genes, we identified the favorable haplotypes with blast resistance, which will provide genetic resources for future rice blast resistance breeding.

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A genome-wide association study of panicle blast resistance to Magnaporthe oryzae in rice

Jinlong,

Yu,

Ruizhi

et al. 2024

Mol Breeding

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Pathogenicity and population structure analysis of Pyricularia oryzae in different districts of Jiangsu province, China

Cited by 7 publications

References 36 publications

Taxonomic and pathogenic diversity of the blast pathogen populations infecting wheat and grasses in Minas Gerais

Taxonomic and pathogenic diversity of the blast pathogen populations infecting wheat and grasses in Minas Gerais

A Genome-wide association study of panicle blast resistance (PBR) to Magnaporthe Oryzae in Rice

A genome-wide association study of panicle blast resistance to Magnaporthe oryzae in rice

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