Pathogenic Virus Detection by Optical Nanobiosensors

Song, Menglin; Yang, Mo; Hao, Jian

doi:10.1016/j.xcrp.2020.100288

Cited by 40 publications

(26 citation statements)

References 87 publications

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“…Elahi et al recently reported the development of a fluorescence DNA probe nano-biosensor method based on iron and gold nanoparticle to identify different species of Shigella 31 . However, this method is more expensive and complicated due to the need for four different probes bound to the gold and iron nanoparticles and the fluorescence spectrophotometer system to measure the fluorescence intensity and characterise possible interactions between the nanoparticles 32 . PCR-HRM is a rapid, simple, and cost-effective assay to detect SNPs in the PCR amplicons of the same size and has been highly recommended by several researchers to be developed and used for differentiation of pathogenic bacterial species isolated from clinical and food samples.…”

Section: Discussionmentioning

confidence: 99%

Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples

Pakbin

Basti

Khanjari

et al. 2022

Sci Rep

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Shigella species, a group of intracellular foodborne pathogens, are the main causes of bacillary dysentery and shigellosis in humans worldwide. It is essential to determine the species of Shigella in outbreaks and food safety surveillance systems. The available immunological and molecular methods for identifying Shigella species are relatively complicated, expensive and time-consuming. High resolution melting (HRM) assay is a rapid, cost-effective, and easy to perform PCR-based method that has recently been used for the differentiation of bacterial species. In this study, we designed and developed a PCR-HRM assay targeting rrsA gene to distinguish four species of 49 Shigella isolates from clinical and food samples and evaluated the sensitivity and specificity of the assay. The assay demonstrated a good analytical sensitivity with 0.01–0.1 ng of input DNA template and an analytical specificity of 100% to differentiate the Shigella species. The PCR-HRM assay also was able to identify the species of all 49 Shigella isolates from clinical and food samples correctly. Consequently, this rapid and user-friendly method demonstrated good sensitivity and specificity to differentiate species of the Shigella isolates from naturally contaminated samples and has the potential to be implemented in public health and food safety surveillance systems.

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Section: Discussionmentioning

confidence: 99%

Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples

Pakbin

Basti

Khanjari

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…They can be used for no pain sample analysis, such as the analysis of patient's saliva using graphene oxide (GO)/Au/Fiber Bragg grating (FBG) probe [298]. Nanotechnology can be used to advance technologies such as paper lateral flow assays [299], label-free biosensors [300][301][302], optical technologies [160,303], and digital technologies [304].…”

Section: Discussionmentioning

confidence: 99%

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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A sensitive method for diagnosing coronavirus disease 2019 (COVID-19) is highly required to fight the current and future global health threats due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV 2). However, most of the current methods exhibited high false-negative rates, resulting in patient misdiagnosis and impeding early treatment. Nanoparticles show promising performance and great potential to serve as a platform for diagnosing viral infection in a short time and with high sensitivity. This review highlighted the potential of nanoparticles as platforms for the diagnosis of COVID-19. Nanoparticles such as gold nanoparticles, magnetic nanoparticles, and graphene (G) were applied to detect SARS-CoV 2. They have been used for molecular-based diagnosis methods and serological methods. Nanoparticles improved specificity and shorten the time required for the diagnosis. They may be implemented into small devices that facilitate the self-diagnosis at home or in places such as airports and shops. Nanoparticles-based methods can be used for the analysis of virus-contaminated samples from a patient, surface, and air. The advantages and challenges were discussed to introduce useful information for designing a sensitive, fast, and low-cost diagnostic method. This review aims to present a helpful survey for the lesson learned from handling this outbreak to prepare ourself for future pandemic.

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“…Conventional detection methods relying on culturing and biochemical analysis are time consuming and take 2 or 3 days or longer [46]. For instance, it is clear that virus cultivation is tedious work, which requires well-trained personnel and specific methodologies [47]. Currently, detection methods for pathogenic bacteria and viruses include polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA).…”

Section: Detection Of Microbial Analytesmentioning

confidence: 99%

“…Currently, detection methods for pathogenic bacteria and viruses include polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Despite many advantages of these methods including high accuracy and decent selectivity, they suffer from drawbacks of being time consuming and labor intensive [47,48]. Moreover, they need expensive instruments and they are not suitable for use in deprived areas [47].…”

Section: Detection Of Microbial Analytesmentioning

confidence: 99%

“…Despite many advantages of these methods including high accuracy and decent selectivity, they suffer from drawbacks of being time consuming and labor intensive [47,48]. Moreover, they need expensive instruments and they are not suitable for use in deprived areas [47]. To overcome the limitations associated with conventional diagnostic methods, alternative robust approaches have been developed for bacteria and virus detection with high sensitivity and accuracy and low detection time.…”

Section: Detection Of Microbial Analytesmentioning

confidence: 99%

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Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

2022

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The lateral flow assay (LFA) is an extensively used paper-based platform for the rapid and on-site detection of different analytes. The method is user-friendly with no need for sophisticated operation and only includes adding sample. Generally, antibodies are employed as the biorecognition elements in the LFA. However, antibodies possess several disadvantages including poor stability, high batch-to-batch variation, long development time, high price and need for ethical approval and cold chain. Because of these limitations, aptamers screened by an in vitro process can be a good alternative to antibodies as biorecognition molecules in the LFA. In recent years, aptamer-based LFAs have been investigated for the detection of different analytes in point-of-care diagnostics. In this review, we summarize the applications of aptamer technology in LFAs in clinical diagnostic rapid tests for the detection of biomarkers, microbial analytes, hormones and antibiotics. Performance, advantages and drawbacks of the developed assays are also discussed.

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Pathogenic Virus Detection by Optical Nanobiosensors

Cited by 40 publications

References 87 publications

Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples

Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

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