PATHOGENIC PROPERTIES OF ^|^ldquo;ENTEROPATHOGENIC^|^rdquo; ESCHERICHIA COLI FROM DIARRHEAL CHILDREN AND ADULTS

Ogawa, Hidemasa; Nakamura, Akiko; Sakazaki, Riichi

doi:10.7883/yoken1952.21.333

Cited by 56 publications

(28 citation statements)

References 17 publications

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“…The penetration of our bovine E. coli isolate differs from that reported for Shigella (II), Yersinia (19), and human isolates of E. coli (12), in that our isolate did not proliferate within the host cells nor induce degeneration of the cells. In the mucosa of the small intestine of guinea pigs, however, cell damage was observed after intraintestinal inoculation with the same isolate, suggesting that local changes occurring in vivo may have been related to factors other than the penetrating ability of the bacteria.…”

Section: Discussioncontrasting

confidence: 81%

“…The enteropathogenicity of E. coli is characterized by distinct properties such as penetration into intestinal epithelial cells (12) and enterotoxigenesis (3,8,13,17) and is related to 0, K and pilus antigens. Some serotypes of 0 and K antigens are isolated more frequently than others from bovine and human cases of diarrhea (5,6,14,15) and certain pilus antigens appear to participate in the expression of the pathogenicity (4, 10).…”

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confidence: 99%

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Protective Effects of Antibody against Intestinal Invasion by Escherichia coli

Okabe

1983

Microbiology and Immunology

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Seven Escherichia coli isolates from newborn calves with diarrhea were examined for enteropathogenic properties. One isolate penetrated into HeLa cells, four produced enterotoxin(s) and the remaining two possessed neither of these properties. Penetration of E. coli into HeLa cells was inhibited by antibody in bovine colostrum and in bovine and rabbit immune sera. The effective antibodies appeared to be mostly of the IgM class. The invasion by E. coli isolates was also examined by inoculation of the bacteria into the small intestine of E. coli-immunized and non-immunized guinea pigs. The isolate which penetrated into HeLa cells could penetrate the intestinal mucosa to be disseminated into various organs of non-immunized guinea pigs but not of immunized guinea pigs, whereas no other isolates showed such pathogenicity in vivo. The inhibition of the invasion was observed when non-immunized guinea pigs were inoculated with the bacteria together with colostral or serum antibody. The results show the importance of antibody in the local defense mechanism against E. coli invasion.Colostral immunoglobulins play a crucial role in the survival of the newborn calf (18) and this situation is not comparable with that in other animal species where maternal immunoglobulins are transmitted to the young during fetal life (1). The newborn calf deprived of colostrum will die of colisepticemia associated with diarrhea soon after birth.As reported previously (16), colostrum contains agglutinins directed against various E. coli serotypes and the spectrum of agglutinin titers varies greatly in colostrum samples from different stock farms, probably reflecting the prevalence of particular serotypes of E. coli. The anti-E. coli activity of these colostrum samples was found mainly in the IgM fraction, when assessed by agglutination and opsonization tests. These results suggested that colostral IgM antibody has an important role in protecting the newborn calf from colibacillosis.The enteropathogenicity of E. coli is characterized by distinct properties such as penetration into intestinal epithelial cells (12) and enterotoxigenesis (3,8,13,17) and is related to 0, K and pilus antigens. Some serotypes of 0 and K antigens are isolated more frequently than others from bovine and human cases of diarrhea (5,6,14,15) and certain pilus antigens appear to participate in the expression of the pathogenicity (4, 10).

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Section: Discussioncontrasting

confidence: 81%

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confidence: 99%

Protective Effects of Antibody against Intestinal Invasion by Escherichia coli

Okabe

1983

Microbiology and Immunology

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“…Microscopy of stained monolayers of HeLa or Henle cells infected by entero-invasive E. coli or Shigella spp. show elongated bacteria scattered throughout the cytoplasm after incubation for 5-8 h and bacterial clumps are not found at an early stage of infection (Gerber and Watkins, 1961 ;Ogawa et al, 1967Ogawa et al, , 1968Calabi, 1970). Marked ultrastructural differences also occur.…”

Section: Discussionmentioning

confidence: 99%

An endocytic process in HEp-2 cells induced by enteropathogenic Escherichia coli

Andrade

Daveiga²,

rosa³

et al. 1989

Journal of Medical Microbiology

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Summary. Infection of HEp-2 cells by enteropathogenic Escherichia coli (EPEC) was examined by transmission and scanning electronmicroscopy. EPEC strains of serogroups 0 1 1 1 : K58 and 055 : K59 recently isolated from human patients did not exhibit enterotoxic activity, as judged by the Vero-cell and suckling-mouse assays, or invasive ability as judged by the Sereny test. These strains attached to and penetrated HEp-2 cells. Transmission electronmicroscopy showed bacteria in close contact with cell membranes 15 min after infection; later, intense swelling and budding of membranes and penetration of EPEC into the cell cytoplasm occurred. Intracellular bacteria were enclosed in membrane-bound vacuoles in the cell cytoplasm underlying localised adherence sites observed by light microscopy. Scanning electronmicroscopy showed morphologically altered membranes only at the sites of bacterial attachment. Bacteria inactivated by ultraviolet light were not internalised and cytochalasin B ( 2 10 mg/L) markedly inhibited uptake. These observations suggest that penetration of EPEC into HEp-2 cells occurs by an endocytic process in metabolically active bacteria.

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“…Thus, the cell invasiveness of these bacilli is believed to be a determinant factor in their pathogenicity. The above process can also be demonstrated in experimental models such as the keratoconjunctival infection system in rabbits or guinea pigs, and the cultured cell-infection system [2,13,18,19,21]. In the present study, virulence of RFPresistant mutants of Shigella and enteropathogenic E. coli were examined, using the infection test of cultured cells and keratoconjunctival infection test (KC-test) in guinea pigs.…”

mentioning

confidence: 87%

“…Regarding pathogenesis of bacillary dysentery, it is well known that penetration of epithelial cells of the intestinal mucous membrane in naturally susceptible host by causative agents, such as Shigella and enteropathogenic E. coli, is an essential step for the establishment of bacillary dysentery [12,13,17,19,21]. Thus, the cell invasiveness of these bacilli is believed to be a determinant factor in their pathogenicity.…”

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confidence: 99%

Virulence of Rifampicin‐Resistant Mutants ofShigellaand EnteropathogenicEscherichia coliwith Special Reference to Their Cell Invasiveness

Osada

Une

Nakajo

et al. 1973

Japanese Journal of Microbiology

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ABSTRACT1. Changes in virulence of Shigella and Shigella-like enteropathogenic Escherichia coli, which occurred during their acquiring resistance to rifampicin were examined. Virulence was evaluated in vitro by their cell-invasive activity, infectivity to the eye of guinea pigs, and LD50 values in intraperitoneally infected mice. 2. The mutants obtained in vivo and those obtained in vitro somewhat varied in cell invasiveness. Ability to produce keratoconjunctivitis in guinea pigs was lacking or diminished in all mutants though the parent strains constantly showed high abilities to invade cultured cells or produce keratoconjuctivitis in guinea pigs. 3. Mutations to resistant strains were not accompanied by any changes in LD50 values in mice.

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PATHOGENIC PROPERTIES OF ^|^ldquo;ENTEROPATHOGENIC^|^rdquo; ESCHERICHIA COLI FROM DIARRHEAL CHILDREN AND ADULTS

Cited by 56 publications

References 17 publications

Protective Effects of Antibody against Intestinal Invasion by Escherichia coli

Protective Effects of Antibody against Intestinal Invasion by Escherichia coli

An endocytic process in HEp-2 cells induced by enteropathogenic Escherichia coli

Virulence of Rifampicin‐Resistant Mutants ofShigellaand EnteropathogenicEscherichia coliwith Special Reference to Their Cell Invasiveness

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