Pathogenic prion protein is degraded by a manganese oxide mineral found in soils

Russo, Fabio; Johnson, Christopher; McKenzie, Debbie; Aiken, Judd M.; Pedersen, Joel A.

doi:10.1099/vir.0.003251-0

Cited by 44 publications

(45 citation statements)

References 34 publications

(40 reference statements)

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“…As reported in literature, a partial recPrP abiotic degradation mediated by MnO 2 could be not excluded [19]. However, in the presence of catechol the degradation of recPrP should have been reduced because birnessite active surfaces were covered by organic catechol polymers [31,32].…”

Section: Discussionmentioning

confidence: 82%

“…While biotic PrP Sc degradation by some proteases can be reduced because of persistent PrP Sc aggregates, its inactivation may arise by abiotic oxidative reactions. In fact birnessite, a manganese oxide common in soil, is able to degrade the PrP Sc in aqueous suspensions [19]. The presence of organic matter coating reactive soil mineral surfaces could hinder abiotic degradation processes with different effects on prion stability.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interaction of a Recombinant Prion Protein with Organo-Mineral Complexes as Assessed by FT-IR and CPMAS 13C NMR Analysis

Russo¹,

Gianfreda²,

Conte³

et al. 2010

TOMRJ

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Prion proteins are considered as the main agents for the Transmissible Spongiform Encephalopathies (TSE). The misfolded form, PrP Sc , which is also indicated as the etiological agent for TSE, exhibits high resistance to degradation in environmental processes. Soil contamination by prion proteins is a real environmental issue since contaminated soils can become potential reservoir and diffuser for TSE infectivity. In this work, the interaction of prion protein with organomineral complexes was studied by using a recombinant non pathogenic prion protein and a model soil system. This latter was represented by a soil manganese mineral coated with polymerized catechol. FT-IR spectra showed amide I and II signals which revealed protein involvement in catechol polymers coating manganese oxide surface. CPMAS 13 C-NMR was applied to follow the complexation of the protein to the soil-like system. All the signals were attributed to C-N in peptidic bonds, alkyl chains and methyl groups. The NMR spectrum of the prion protein interacting directly with birnessite revealed disappearance of signals due to the paramagnetic nature of manganese oxide or protein abiotic degradation, while the presence of organic matter strongly reduced the disappearance of prion protein signals.

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Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Interaction of a Recombinant Prion Protein with Organo-Mineral Complexes as Assessed by FT-IR and CPMAS 13C NMR Analysis

Russo¹,

Gianfreda²,

Conte³

et al. 2010

TOMRJ

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show abstract

“…50 A dramatic increase in PrP Sc survival (10×) over a two year period was observed in the presence of manganese-ions in a soil solution. 22 Mn-associated prions are 100 times more likely to cause infection than a "metalfree" prion. 21 We have recently shown that low levels of copper and high level of manganese also were associated with abnormal prion protein in the brains of infected animals.…”

Section: Metals In Soilmentioning

confidence: 99%

“…To date, only a few soil characteristics have been investigated with respect to their influence on infectious and uninfected agents: some common soil minerals (montomorillonite, kaolinite and quartz), [14][15][16] organic polyanions and humic acids (as soil organic matter compounds), [17][18][19][20] and some metals and their oxides (Al 2 O 3 , SiO 2 , MnO). [21][22][23][24][25] The main focus of this review is the characterization of soil properties in the CWD-endemic region and its surrounding areas from Alberta to Manitoba to estimate their potential for binding prions and maintaining bioavailability.…”

Section: Introductionmentioning

confidence: 99%

Potential role of soil properties in the spread of CWD in western Canada

Kuznetsova

McKenzie

Banser

et al. 2014

Prion

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“…[27][28][29][30] However, other soil characteristics, such as natural oxidants like manganese oxides (MnO 2 ), appear to interfere with the conversion of PrP C to PrP Sc . 31 To our knowledge there is no data regarding the concentration of Mn, Cu, Fe and Mg in obex and RLN of white-tailed deer even though these tissues are the preferred sites for diagnostic sampling and testing for CWD, and target sites for PrP Sc accumulation during infection. The objectives of this study were: 1) to compare concentrations of Cu, Fe, Mn and Mg between tissues (obex and RLN) and CWD status, 2) to establish reference intervals (RIs) for Cu, Mn, Fe and Mg in obex and RLN of Illinois free-ranging white-tailed deer; and 3) to explore the utility of the reference intervals established in objective 2 for discriminating between CWD-positive and CWD-negative animals.…”

Section: Introductionmentioning

confidence: 99%

Metals in obex and retropharyngeal lymph nodes of Illinois white-tailed deer and their variations associated with CWD status

Rivera

Novakofski

Weng

et al. 2015

Prion

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ABSTRACT. Prion proteins (PrP C ) are cell membrane glycoproteins that can be found in many cell types, but specially in neurons. Many studies have suggested PrP C 's participation in metal transport and cellular protection against stress in the central nervous system (CNS). On the other hand PrP Sc , the misfolded isoform of PrP C and the pathogenic agent in transmissible spongiform encephalopathies (TSE), has been associated with brain metal dyshomeostasis in prion diseases. Thus, changes in metal concentration associated with protein misfolding and aggregation have been reported for human and animal prion diseases, as well as for other neurodegenerative disorders, such as Parkinson's and Alzheimer's disease. The use of metal concentrations in tissues as surrogate markers for early detection of TSEs has been suggested. Studies on the accumulation of metals in free-ranging white-tailed deer have not been conducted. This study established concentrations of copper, iron, manganese, and magnesium in 2 diagnostic tissues used for CWD testing (obex and retropharyngeal lymph nodes (RLN)). We compared these concentrations between tissues and in relation to CWD status. We established reference intervals (RIs) for these metals and explored their ability to discriminate between CWD-positive and CWD-negative animals. Our results indicate that independent of CWD status, white-tailed deer accumulate higher concentrations of Fe, Mn and Mg in Ó Nelda A Rivera, Jan Novakofski, Hsin-Yi Weng, Amy Kelly, Damian Satterthwaite-Phillips, Marilyn O Ruiz, and Nohra Mateus-Pinilla *Correspondence to: Nohra Mateus-Pinilla; Email:nohram@illinois.edu Received November 21, 2014; Revised January 25, 2015; Accepted February 9, 2015. Color versions of one or more figures in this article can be found online at www.tandfonline.com/kprn. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. ISSN: 1933-6896 print / 1933-690X online DOI: 10.1080/19336896.2015 RLN than in obex. White-tailed deer infected with CWD accumulated significantly lower concentrations of Mn and Fe than CWD-negative deer. These patterns differed from other species infected with prion diseases. Overlapping values between CWD positive and negative groups indicate that evaluation of these metals in obex and RLN may not be appropriate as a diagnostic tool for CWD infection in white-tailed deer. Because the CWD-negative deer were included in constructing the RIs, high specificities were expected and should be interpreted with caution. Due to the low sensitivity derived from the RIs, we do not recommend using metal concentrations for disease discrimination.Prion, 9:48-58, 2015 Published with license by Taylor & Francis Group, LLC

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Pathogenic prion protein is degraded by a manganese oxide mineral found in soils

Cited by 44 publications

References 34 publications

Interaction of a Recombinant Prion Protein with Organo-Mineral Complexes as Assessed by FT-IR and CPMAS 13C NMR Analysis

Interaction of a Recombinant Prion Protein with Organo-Mineral Complexes as Assessed by FT-IR and CPMAS 13C NMR Analysis

Potential role of soil properties in the spread of CWD in western Canada

Metals in obex and retropharyngeal lymph nodes of Illinois white-tailed deer and their variations associated with CWD status

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