Pathogenic potential and virulence genotypes of intestinal and faecal isolates of porcine post-weaning enteropathogenic Escherichia coli

Malik, Anna; Nagy, Béla; Kugler, Renáta; Szmolka, Ama

doi:10.1016/j.rvsc.2017.02.002

Cited by 15 publications

(16 citation statements)

References 64 publications

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“…However, though not routinely screened for, aEPEC (eae+, stx−, bfp−) are also known to cause enteric colibacillosis in swine (Fairbrother et al, 2005;Luppi, 2017) and García-Meniño et al (2018) found aEPEC outnumbered ETEC as the causative agents in suckling piglets. Nevertheless, eae+ E. coli has been isolated from both healthy and diarrheic pigs (Krause et al, 2005;Fröhlicher et al, 2008;Malik et al, 2017), implying that a combination of additional VAGs, as well as host-related factors, and poor herd management and husbandry practices, are required to establish disease.…”

Section: Discussionmentioning

confidence: 99%

“…aEPEC from sequence type (ST) 302 (serotype O108:H9) have been isolated from both healthy and diseased hosts, and the environment. They have been isolated from healthy pigs (Fröhlicher et al, 2008;Malik et al, 2017;Reid et al, 2017), water tanks in a poultry slaughterhouse (Alonso et al, 2014) and pork products made for human consumption (Lugsomya et al, 2018), and have been associated with diarrheic rabbits , pigs (Kleta et al, 2014), and humans (Foster et al, 2015). Yet to date, no genomic comparisons of E. coli ST302 strains, nor a complete ST302 genome, has been published.…”

Section: Introductionmentioning

confidence: 99%

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Escherichia coli ST302: Genomic Analysis of Virulence Potential and Antimicrobial Resistance Mediated by Mobile Genetic Elements

et al. 2020

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aEPEC are associated with persistent diarrhea, and diarrheal outbreaks in both humans and animals worldwide. They are differentiated from typical EPEC by the lack of bundleforming pili, and from EHEC by the lack of phage-mediated stx toxins. However, phylogenetic analyses often associate aEPEC with EHEC, promoting the hypothesis that aEPEC are the progenitors of EHEC, which is supported by aEPEC conversion to EHEC by stx-carrying phages. While aEPEC can cause disease outright, the potential to acquire stx, one of the most potent bacterial toxins known, merits close monitoring. Escherichia coli ST302 (O108:H9, O182:H9, O45:H9) are aEPEC that have been isolated from diarrheic human, pig and rabbit hosts, as well as in healthy pigs, however, no study to date has focused on E. coli ST302 strains. Through WGS and hybrid assembly we present the first closed chromosome, and two circularized plasmids of an ST302 strain-F2_18C, isolated from a healthy pig in Australia. A phylogenetic analysis placed E. coli ST302 strains in proximity to EHEC ST32 (O145:H28) strains. Public databases were interrogated for WGSs of E. coli ST302 strains and shortread gene screens were used to compare their virulence-associated gene (VAG) and antimicrobial resistance gene (ARG) cargo. E. coli ST302 strains carry diverse VAGs, including those that typically associated with extraintestinal pathogenic E. coli (ExPEC). Plasmid comparisons showed that pF2_18C_FIB shared homology with EHEC virulence plasmids such as pO103 while pF2_18C_HI2 is a large multidrug resistance IncHI2:ST3 plasmid. A comparison of 33 HI2:ST3 plasmids demonstrated that those of Australian origin have not acquired resistances to extended-spectrum beta-lactams, colistin, fosfomycin or rifampicin, unlike those originating from Asia. F2_18C was shown to carry two additional pathogenicity islands-ETT2, and the STEC-associated PAI CL3 , plasmid-associated heavy metal resistance genes, as well as several unoccupied stxphage attachment sites. This study sheds light on the virulence and AMR potential of E. coli ST302 strains and informs AMR genomic surveillance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Escherichia coli ST302: Genomic Analysis of Virulence Potential and Antimicrobial Resistance Mediated by Mobile Genetic Elements

et al. 2020

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“…For atypical EPEC, both animals and humans can be reservoirs and are known as pathogenic for children and young animals ( Trabulsi et al, 2002 ; Watson et al, 2017 ). aEPEC have also been implicated in PWD in pigs but their pathogenicity still remains unclear ( Malik et al, 2017 ). According to these and other authors, there exist differences of serotypes and intimin types among porcine eae -positive isolates in relation to the pathogenic potential, such as sero/intimin type O123:H11/ eae-β1 or O45/ eae-β1 (both determined in our collection) with AE activity on ileal villi and frequently occurring in diarrheagenic pigs ( Zhu et al, 1994 ; Malik et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…aEPEC have also been implicated in PWD in pigs but their pathogenicity still remains unclear ( Malik et al, 2017 ). According to these and other authors, there exist differences of serotypes and intimin types among porcine eae -positive isolates in relation to the pathogenic potential, such as sero/intimin type O123:H11/ eae-β1 or O45/ eae-β1 (both determined in our collection) with AE activity on ileal villi and frequently occurring in diarrheagenic pigs ( Zhu et al, 1994 ; Malik et al, 2017 ). In previous studies on swine diarrhea in Slovakia, we had found a much lower aEPEC involvement (3.2 and 0.9% of neonatal and PWD isolates, respectively) ( Vu Khac et al, 2006 , Vu Khac et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

“…Other associated fimbriae of lower prevalence include F5 (K99), F6 (987P), and F41, whose number of active receptors present on the intestinal epithelial cells decreases with age ( Vu Khac et al, 2006 ; Luppi et al, 2016 ). Enteropathogenic E. coli (EPEC) is another pathotype found in pigs with enteric colibacillosis ( Brand et al, 2017 ; Malik et al, 2017 ) which was first associated with diarrhea in infants ( Shuman and Stock, 1956 ). EPEC isolates possess an outer membrane protein adhesin or intimin (Eae), responsible for intimate attachment of the bacteria to the host intestinal epithelium, which together with a complex secretion system leads to the development of the “attaching and effacing” (AE) lesion ( Frankel and Phillips, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

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Swine Enteric Colibacillosis in Spain: Pathogenic Potential of mcr-1 ST10 and ST131 E. coli Isolates

et al. 2018

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This is a wide epidemiological study of 499 E. coli isolates recovered from 179 outbreaks of enteric colibacillosis from pig production farms in Spain during a period of 10 years. Most samples were of diarrheagenic cases occurred during the post-wean period (PWD) which showed to be significantly associated with ETEC (67%) followed by aEPEC (21.7%). On the contrary, aEPEC was more prevalent (60.3%) among diarrheas of suckling piglets, followed by ETEC (38.8%). STEC/ETEC or STEC were recovered in 11.3 and 0.9% of PWD and neonatal diarrhea, respectively. Detection of the F4 colonization factor was not significantly different between isolates recovered from neonatal pigs and those recovered post wean (40.5 versus 27.7%) while F18 was only present among PWD isolates (51.5% of ETEC, STEC, and STEC/ETEC isolates). We also found a high prevalence of resistance to colistin related to the presence of the mcr-1 gene (25.6% of the diarreagenic isolates). The characterization of 65 representative mcr-1 isolates showed that all were phenotypically resistant to colistin (>2 μg/ml), and most (61 of 65) multidrug-resistant (MDR). Six ETEC and one STEC mcr-1 isolates were also carriers of ESBL genes. In addition, other seven mcr-1 isolates harbored mcr-4 (three ETEC) and mcr-5 (two ETEC and two aEPEC) genes. In the phylogenetic analysis of the 65 mcr-1 diarrheagenic isolates we found that more than 50% (38 out of 65) belonged to A-ST10 Cplx and from those, 29 isolates showed the clonotype CH11-24. In this study, we also recovered 18 ST131 isolates including seven mcr-1 carriers. To the best of our knowledge, this would be the first report of ST131 mcr-1 isolation in pigs. Worryingly, the swine mcr-1 ST131 carriers also showed MDR, including to trimethoprim-sulfamethoxazole, tobramycin, gentamicin and ciprofloxacin. In the PFGE-macrorestriction comparison of clinical swine and human ST131, we found high similarities (≥85%) between two pig and two human ST131 isolates of virotype D5. Acquisition of mcr-1 by this specific clone means an increased risk due to its special feature of congregating virulence and resistance traits, together with its spread capability. Here we show a potential zoonotic swine source of ST131.

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Metagenomic and transcriptomic analysis revealing the impact of oxytetracycline and ciprofloxacin on gut microbiota and gene expression in the Chinese giant salamander (Andrias davidianus)

Ye,

He,

et al. 2024

Aquatic Toxicology

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Pathogenic potential and virulence genotypes of intestinal and faecal isolates of porcine post-weaning enteropathogenic Escherichia coli

Cited by 15 publications

References 64 publications

Escherichia coli ST302: Genomic Analysis of Virulence Potential and Antimicrobial Resistance Mediated by Mobile Genetic Elements

Escherichia coli ST302: Genomic Analysis of Virulence Potential and Antimicrobial Resistance Mediated by Mobile Genetic Elements

Swine Enteric Colibacillosis in Spain: Pathogenic Potential of mcr-1 ST10 and ST131 E. coli Isolates

Metagenomic and transcriptomic analysis revealing the impact of oxytetracycline and ciprofloxacin on gut microbiota and gene expression in the Chinese giant salamander (Andrias davidianus)

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