Pathogenic potential and genotypic diversity of Campylobacter jejuni: a neglected food-borne pathogen in Brazil

Frazão, Miliane Rodrigues; Medeiros, Marta Inês Cazentini; Duque, Sheila da Silva; Falcão, Juliana Pfrimer

doi:10.1099/jmm.0.000424

Cited by 20 publications

(26 citation statements)

References 61 publications

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“…We noted that all Campylobacter isolates were positive for the flaA and flhA genes, regardless of the species and sample origin. These findings are in accordance with data presented previously in Poland [13], Vietnam [40], Brazil [41], and Denmark [42]. Besides, the above authors reported on Campylobacter isolates obtained from chicken or turkey.…”

Section: Discussionsupporting

confidence: 93%

“…The dnaJ, racR, and docA genes were commonly distributed in Campylobacter strains in different geographic regions. Frazao et al [41] showed the prevalence of these genes in all isolates from poultry feces and carcasses in Brazil. Additionally, Wieczorek et al [49] showed the high prevalence of the docA and racR genes in 100% Campylobacter strains isolated from chicken feces and in 98.1% and 95.5% of the isolates from poultry carcasses, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Resistance and Virulence Properties of Campylobacter Spp. Originating from Domestic Geese in Poland

Wysok

Wojtacka

Wiszniewska-Łaszczych

et al. 2020

Animals

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A total of 240 samples were evaluated for the presence of Campylobacter spp. Campylobacter was found in 83.3% of the cecum contents samples and 52.5% of the neck skin samples from carcasses. The prevailing species was C. jejuni, accounting for 87.7% of all Campylobacter isolates, and the remaining 12.3% of isolates were C. coli. All Campylobacter isolates, independent of the sample origin and species, were positive for 6 out of 15 tested genes (flaA, flhA, cadF, racR, ciaB, and cdtA genes). The prevalence of dnaJ, docA, pldA, cdtB, cdtC, and iam genes was also very common (ranging from 86.5% to 98.8%). The lowest prevalence was noted for virB11 and wlaN genes, both in Campylobacter isolates from cecum (12% and 19%) and carcasses (11.1% and 17.5%). None of the isolates tested, regardless of the sample origin, carried the cgtB gene. The highest resistance rates were observed for quinolones (90.8%) and tetracyclines (79.8%). Simultaneously, only single Campylobacter isolate was resistant to macrolides (0.6%) and none of the isolates showed resistance to aminoglycosides and amphenicols. The common presence of Campylobacter on geese carcasses as well as the detection of multidrug-resistant isolates indicate that consuming goose meat might cause a potential risk, therefore leading to human campylobacteriosis.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Virulence Properties of Campylobacter Spp. Originating from Domestic Geese in Poland

Wysok

Wojtacka

Wiszniewska-Łaszczych

et al. 2020

Animals

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“…pld A gene encoding for outer membrane phospholipase A and involved in host cell invasion was investigated and found positive for 26.25% of the Campylobacter isolates. Previous studies reported a high prevalence of pld A gene in Campylobacter isolates such as 100% for pld A gene was reported by Frazão et al, . Relatively low prevalence (11.25%) of vir B11 gene was found in C. jejuni which was in accordance with previous reports (González‐Hein, Huaracán, García, & Figueroa, ; Wieczorek, Szewczyk, & Osek, ).…”

Section: Discussionsupporting

confidence: 90%

“…C. jejuni secretes a protein which is essential for the invasion of cultured epithelial cells, called Cia B (Konkel et al, ). Campylobacter isolates (76.5%) were found positive for cia B gene, whereas, Frazão, Medeiros, da Silva Duque, & Falcão, reported all Campylobacter isolates positive for cia B gene.…”

Section: Discussionmentioning

confidence: 90%

Prevalence, antimicrobial susceptibility, and virulence of Campylobacter jejuni isolated from chicken meat

Samad

Abbas

Ahmed

et al. 2018

Journal of Food Safety

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The aim of this study was to determine the prevalence and antimicrobial resistance of Campylobacter jejuni isolated from retail chicken meat. The identification of Campylobacter isolates and the presence of virulence factor were evaluated by polymerase chain reaction (PCR). Furthermore, clove oil, cinnamon, and turmeric extracts were evaluated for the antimicrobial potential against Campylobacter isolates. Out of 200 chicken meat samples, 80 (40%) samples were found contaminated with Campylobacter jejuni. Antibacterial susceptibility testing indicated that out of 80 isolates 60 (75%) were resistant to tetracycline followed by 31 (38.75%) to ciprofloxacin, 12 (15%) to ampicillin, 8 (10%) to erythromycin, and 2 (2.5%) were resistant to chloramphenicol. Clove oil and cinnamon extract showed antibacterial potential against Campylobacter isolates. Furthermore, all the 80 isolates (100%) were found positive for virulence genes (cadF, flaA, and dnaJ). The presence of antibacterial resistance and virulence factors in C. jejuni highlighted the risk associated with retail poultry meat. Practical applications Campylobacter jejuni is associated with foodborne illnesses such as gastrointestinal intestinal complications. This study demonstrated that raw chicken meat should be subjected to pretreatment to avoid the foodborne illnesses associated with multidrug‐resistant (MDR) Campylobacter jejuni. Moreover, the use of antibiotics should be strictly monitored in developing countries to avoid the emergence of multidrug‐resistant pathogens.

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“…DNA from each strain was extracted according to Frazão et al (4). Libraries were prepared using 1 ng of genomic DNA with the Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of 116 Campylobacter jejuni Strains Isolated from Humans, Animals, Food, and the Environment in Brazil

et al. 2018

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Pathogenic potential and genotypic diversity of Campylobacter jejuni: a neglected food-borne pathogen in Brazil

Cited by 20 publications

References 61 publications

Antimicrobial Resistance and Virulence Properties of Campylobacter Spp. Originating from Domestic Geese in Poland

Antimicrobial Resistance and Virulence Properties of Campylobacter Spp. Originating from Domestic Geese in Poland

Prevalence, antimicrobial susceptibility, and virulence of Campylobacter jejuni isolated from chicken meat

Draft Genome Sequences of 116 Campylobacter jejuni Strains Isolated from Humans, Animals, Food, and the Environment in Brazil

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