Pathogenic infection confounds induction of the estrogenic biomarker vitellogenin in rainbow trout

Burki, Richard; Krasnov, Aleksei; Bettge, Kathrin; Rexroad, Caird E.; Afanasyev, Sergey; Antikainen, Mia; Burkhardt‐Holm, Patricia; Wahli, Thomas; Segner, Helmut

doi:10.1002/etc.1966

Cited by 8 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As an endogenous reference, 18S rRNA (Applied Biosystems, Foster City, CA, USA) was measured for the quality check of reverse-transcription of each cDNA. The gene expression level of liver-vitellogenin (VTG, Hamburg, Germany) [ 68 ] was utilized as an indicator for E2 response.…”

Section: Methodsmentioning

confidence: 99%

Immune-Specific Expression and Estrogenic Regulation of the Four Estrogen Receptor Isoforms in Female Rainbow Trout (Oncorhynchus mykiss)

Casanova-Nakayama

Siebenthal

Kropf

et al. 2018

IJMS

Self Cite

View full text Add to dashboard Cite

Genomic actions of estrogens in vertebrates are exerted via two intracellular estrogen receptor (ER) subtypes, ERα and ERβ, which show cell- and tissue-specific expression profiles. Mammalian immune cells express ERs and are responsive to estrogens. More recently, evidence became available that ERs are also present in the immune organs and cells of teleost fish, suggesting that the immunomodulatory function of estrogens has been conserved throughout vertebrate evolution. For a better understanding of the sensitivity and the responsiveness of the fish immune system to estrogens, more insight is needed on the abundance of ERs in the fish immune system, the cellular ratios of the ER subtypes, and their autoregulation by estrogens. Consequently, the aims of the present study were (i) to determine the absolute mRNA copy numbers of the four ER isoforms in the immune organs and cells of rainbow trout, Oncorhynchus mykiss, and to compare them to the hepatic ER numbers; (ii) to analyse the ER mRNA isoform ratios in the immune system; and, (iii) finally, to examine the alterations of immune ER mRNA expression levels in sexually immature trout exposed to 17β-estradiol (E2), as well as the alterations of immune ER mRNA expression levels in sexually mature trout during the reproductive cycle. All four ER isoforms were present in immune organs—head kidney, spleen-and immune cells from head kidney and blood of rainbow trout, but their mRNA levels were substantially lower than in the liver. The ER isoform ratios were tissue- and cell-specific, both within the immune system, but also between the immune system and the liver. Short-term administration of E2 to juvenile female trout altered the ER mRNA levels in the liver, but the ERs of the immune organs and cells were not responsive. Changes of ER gene transcript numbers in immune organs and cells occurred during the reproductive cycle of mature female trout, but the changes in the immune ER profiles differed from those in the liver and gonads. The correlation between ER gene transcript numbers and serum E2 concentrations was only moderate to low. In conclusion, the low mRNA numbers of nuclear ER in the trout immune system, together with their limited estrogen-responsiveness, suggest that the known estrogen actions on trout immunity may be not primarily mediated through genomic actions, but may involve other mechanisms, such as non-genomic pathways or indirect effects.

show abstract

Section: Methodsmentioning

confidence: 99%

Immune-Specific Expression and Estrogenic Regulation of the Four Estrogen Receptor Isoforms in Female Rainbow Trout (Oncorhynchus mykiss)

Casanova-Nakayama

Siebenthal

Kropf

et al. 2018

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ethinylestradiol (EE2), an anthropogenic stressor, was used as the contaminant, as it is widespread in freshwater ecosystems. In juvenile fish, EE2 induces the synthesis of vitellogenin, which is the precursor of egg yolk, cost-intensive [44][45][46], and can lead to immune and growth-modulating effects [47,48]. The pathogen that was selected for this study was the myxozoan parasite Tetracapsuloides bryosalmonae, which is the causative agent of proliferative kidney disease (PKD) in salmonids [49,50].…”

Section: Introductionmentioning

confidence: 99%

Trade-Offs Underwater: Physiological Plasticity of Rainbow Trout (Oncorhynchus mykiss) Confronted by Multiple Stressors

Siebenthal

Rehberger

Bailey

et al. 2018

Fishes

Self Cite

View full text Add to dashboard Cite

Organisms have evolved mechanisms to partition the available resources between fitness-relevant physiological functions. Organisms possess phenotypic plasticity to acclimate to changing environmental conditions. However, this comes at a cost that can cause negative correlations or “trade-offs”, whereby increasing investments in one function lead to decreased investments in another function. The aim of the present study was to investigate the prioritization of resource allocation between growth, pathogen defense, and contaminant response in juvenile rainbow trout (Oncorhynchus mykiss) exposed to changes of resource income or expenditure. We performed a multifactorial experiment with three resource-impacting stressors—limited food availability, a parasitic infection, exposure to a vitellogenesis-inducing contaminant—and combinations thereof. Treatment with the individual stressors evoked the expected responses in the respective physiological target systems—body growth, immune system, and hepatic vitellogenin transcription—but we found little evidence for significant negative relations (trade-offs) between the three systems. This also applied to fish exposed to combinations of the stressors. This high phenotypic flexibility of trout in their resource allocation suggests that linear resource allocations as mechanisms of phenotypic plasticity may be too simplistic, but it also may point to a greater capacity of ectothermic than endothermic vertebrates to maintain key physiological processes under competing resource needs due to lower maintenance costs.

show abstract

“…The endocrine functions are one of the important physiological features that play an important role in reproduction and recruitment of a fish species akin to that of vertebrate reproduction. Vitellogenin (Vtg) is considered as an important physiological indicator of endocrine functional disruptions (Hutchinson et al, 2009;Burki et al, 2012;Wheeler et al, 2013). The process of reproductive disruption is the result of hormonal alterations that are due to either natural causes or due to anthropological activities.…”

Section: Introductionmentioning

confidence: 99%

Induction of vitellogenin in cultured fish: Vitellogenin gene expression response of blue fin and yellow fin breams to injected 17 -estradiol

Azad¹,

Al-Jandal²

2020

J. Environ. Chem. Ecotoxicol.

View full text Add to dashboard Cite

Aquatic organisms in the coastal regions are often exposed to many anthropogenic effluents. Kuwait Bay receives considerable quantities of sewage and industrial effluents, and thus, naturally, many endocrine disrupting chemicals (EDCs) find their way into the coastal waters. These EDCs could influence the physiology of the aquatic organisms inhabiting the coastal region. Response of two important local fish of aquaculture importance, bluefin bream (Sparidentax hasta) and yellowfin bream (Acanthopagrus latus) was evaluated by injecting a standard vitellogenin inducer, 17 β-estradiol (17 BEST). The inducer was used at 2, 4 and 6 µg/fish (injected intraperitoneally) to induce vitellogenin production in juvenile fish (150-200 g). There was a dose-dependent Vtg induction response as evidenced by a qualitative immune-dot assay. All levels significantly enhanced Vtg gene expression, as early as one-hour post injection. The expression was noticed to be the highest in liver tissue at 6 h post injection. The bluefin bream was used for amplification of the Vtg gene yielding 282 bp product which was sequenced. This indicated a 97% sequence similarity with the Vtg gene sequence of European seabream, S. aurata.

show abstract

Pathogenic infection confounds induction of the estrogenic biomarker vitellogenin in rainbow trout

Cited by 8 publications

References 30 publications

Immune-Specific Expression and Estrogenic Regulation of the Four Estrogen Receptor Isoforms in Female Rainbow Trout (Oncorhynchus mykiss)

Immune-Specific Expression and Estrogenic Regulation of the Four Estrogen Receptor Isoforms in Female Rainbow Trout (Oncorhynchus mykiss)

Trade-Offs Underwater: Physiological Plasticity of Rainbow Trout (Oncorhynchus mykiss) Confronted by Multiple Stressors

Induction of vitellogenin in cultured fish: Vitellogenin gene expression response of blue fin and yellow fin breams to injected 17 -estradiol

Contact Info

Product

Resources

About