PATHOGENIC <i>NAEGLERIA</i> AND <i>HARTMANNELLA (ACANTHAMOEBA)</i>

Culbertson, C. G.

doi:10.1111/j.1749-6632.1970.tb45623.x

Cited by 24 publications

(16 citation statements)

References 17 publications

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“…Naegleria fowleri is a ubiquitous pathogenic free-living amoeba and has been isolated from samples obtained from chlorinated swimming pools, freshwater lakes, hot springs, domestic water supplies, thermally polluted waters, sewage, soil, air, humidified environment, cell culture, and nasal cavities (Culbertson 1970). It also exists as a virulent pathogen causing fatal primary amoebic meningoencephalitis (PAME) in experimental animal, human, and other mammals (Carter 1968).…”

Section: Introductionmentioning

confidence: 98%

The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri

et al. 2010

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Naegleria fowleri destroys target cells by trogocytosis, a phagocytosis mechanism, and a process of piecemeal ingestion of target cells by food-cups. Phagocytosis is an actin-dependent process that involves polymerization of monomeric G-actin into filamentous F-actin. However, despite the numerous studies concerning phagocytosis, its role in the N. fowleri food-cup formation related with trogocytosis has been poorly reported. In this study, we cloned and characterized an Nf-actin gene to elucidate the role of Nf-actin gene in N. fowleri pathogenesis. The Nf-actin gene is composed of 1,128-bp and produced a 54.1-kDa recombinant protein (Nf-actin). The sequence identity was 82% with nonpathogenic Naegleria gruberi but has no sequence identity with other mammals or human actin gene. Anti-Nf-actin polyclonal antibody was produced in BALB/c mice immunized with recombinant Nf-actin. The Nf-actin was localized on the cytoplasm, pseudopodia, and especially, food-cup structure (amoebastome) in N. fowleri trophozoites using immunofluorescence assay. When N. fowleri co-cultured with Chinese hamster ovary cells, Nf-actin was observed to localize around on phagocytic food-cups. We also observed that N. fowleri treated with cytochalasin D as actin polymerization inhibitor or transfected with antisense oligomer of Nf-actin gene had shown the reduced ability of food-cup formation and in vitro cytotoxicity. Finally, it suggests that Nf-actin plays an important role in phagocytic activity of pathogenic N. fowleri.

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Section: Introductionmentioning

confidence: 98%

The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri

et al. 2010

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“…The organisms are commonly found in soil and in warm bodies of fresh water such as lakes, rivers, hot springs, and unchlorinated swimming pools and in warm water discharge pools from industrial plants (Culbertson 1970). PAME is a fulminant infection that typically leads to death with 1 to 2 weeks from the onset of symptoms.…”

Section: Introductionmentioning

confidence: 99%

Immunodominant antigens in Naegleria fowleri excretory–secretory proteins were potential pathogenic factors

et al. 2009

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Naegleria fowleri, a ubiquitous pathogenic freeliving amoeba, is the most virulent species and causes primary amoebic meningoencephalitis in laboratory animals and humans. The parasite secretes various inducing molecules as biological responses, which are thought to be involved in pathophysiological and immunological events during infection. To investigate what molecules of N. fowleri excretory-secretory proteins (ESPs) are related with amoebic pathogenicity, N. fowleri ESPs fractionated by two-dimensional electrophoresis were reacted with N. fowleri infection or immune sera. To identify immunodominant ESPs, six major protein spots were selected and analyzed by N-terminal sequencing. Finally, six proteins, 58, 40, 24, 21, 18, and 16 kDa of molecular weight, were partially cloned and matched with reference proteins as follow: 58 kDa of exendin-3 precursor, 40 kDa of secretory lipase, 24 kDa of cathepsin B-like proteases and cysteine protease, 21 kDa of cathepsin B, 18 kDa of peroxiredoxin, and 16 kDa of thrombin receptor, respectively. These results suggest that N. fowleri ESPs contained important proteins, which may play an important role in the pathogenicity of N. fowleri.

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“…Thermophilic N. fowleri trophozoites grow at temperatures of between 30 and 37°C and can tolerate temperatures up to 45°C (3). N. fowleri has also been found in moist soil, lakes, rivers, hot springs, swimming pools, and pools of warm water discharged from industrial plants (1,4).…”

mentioning

confidence: 99%

“…It has a worldwide distribution and causes an acute primary amoebic meningoencephalitis (PAM) and an acute and severe central nervous system (CNS) disease in experimental animals and humans (1)(2)(3) and is becoming a serious issue in subtropical and tropical countries as a neglected tropical disease (NTD) (4,5). Depending on the environmental conditions, the life cycle of N. fowleri has three stages: the cyst, trophozoite, and flagellate stages.…”

mentioning

confidence: 99%

NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri

et al. 2016

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PATHOGENIC NAEGLERIA AND HARTMANNELLA (ACANTHAMOEBA)

Cited by 24 publications

References 17 publications

The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri

The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri

Immunodominant antigens in Naegleria fowleri excretory–secretory proteins were potential pathogenic factors

NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri

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