Pathogenic Anaerobic Bacteria

Miraglia, Gennaro J.

doi:10.3109/10408417409108749

Cited by 10 publications

(4 citation statements)

References 103 publications

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“…Within the past year several different experimental animal models of "bacteroides" infection have been developed (11,15,23,24). Hill et al (11) produced progressive hepatic abscesses Organ distribution of F. necrophorum in immunized mice after i.p.…”

Section: Discussionmentioning

confidence: 99%

Fusobacterium necrophorum infection in mice as a model for the study of liver abscess formation and induction of immunity

Abe¹,

Lennard²,

Holland³

1976

Infect Immun

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A mouse model is described in which intraperitoneal injection of Fusobacterium necrophorum results in chronic liver abscesses. Viable bacterial counts from mouse lung, liver, and spleen were obtained after whole organ homogenization. From 2 h to 5 days postchallenge, liver was found to contain more bacteria than lung on a per gram basis. Bacterial counts from liver and spleen were about the same during the first 8 h; thereafter liver was found to contain more bacteria. By day 13, though bacterial counts were equivalent in the three organs, abscesses were only observed in liver. This predilection for the liver may be due to a nutritional and/or microenvironmental factor(s). Blood cultures of infected mice revealed a general lack of bacteremia. Extended immunization with formalin-killed cells was found to protect mice against F. necrophorum infection.

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Section: Discussionmentioning

confidence: 99%

Fusobacterium necrophorum infection in mice as a model for the study of liver abscess formation and induction of immunity

Abe¹,

Lennard²,

Holland³

1976

Infect Immun

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“…Tryptiease soy blood agar plates that were streaked anaerobically and transferred to Brewer jars inside the glove box (no evacu.ation) gave lower counts than those streaked aerobically and placed in jars outside the box. This suggests that unless the jar is thoroughly evacuated before being filled with gas mixture residual oxygen may be left, MIEAGLIA (22), in a review on techniques and instrumentation for anaerobic bacteria, thought the data of KiLLGORE.ri a/, (15) and ROSENBLATT et al (28), purporting to show the superiority of the jar procedure over that of the glove box, would have been more convincing if thejars had not been used in com bination with the box. The present results suggest that such a combination actually may be a disadvantage in the jar technique.…”

Section: Discussionmentioning

confidence: 99%

Comparison of there anaerobic culture techniques and media for viable recovery of subgingival plaque bacteria

Olsen¹,

Socransky²

1981

European J Oral Sciences

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– Various methods are available for viable recovery of bacteria from subgingival plaque, but theire relative efficicency is not clear. In the first experiment, with 10 patients, three anaerobic techniques (Brewer jars, chamber and roll tubes) and three agar media (trypicase soy with 5% sheep blood (TS), brucella agar with 5% blood and 0.5 ug/ml of menadione (B), and modified medium 10 with 3% blood (MM 10)), were compared. In the second experiment, with anaother 10 patients, Brewer jars were compared with a glove box, using TS. Subgingival plaque was sampled with a gas flushed broach passed through a syringe. Significance of differences in viale recoveries was determined by ANOVA. Brewer jars and chamber (aerobic plating) were comparable in efficiency, but excelled roll tubes (anaerobic inoculation). TS was better than B, but equivalent to MM 10. TS in Brewer jars (aerobic plating) matched TS in the glove box (anaerobic plating), but perforemed better than all the other procedures tested. Recoveries did not differ on pre‐and non‐prereduced plates and on commercial and laboratory, freshly made platels. Non‐evacuated jars sealed inside the box (anaerobic plating) provided lower recoveries than jars evacuated, filled and sealed outside (aerobic plating). Roll tubes benefited most by extending incubation from 7 to 14d. Commercial, non‐prereduced TS agar plates incubated in Brewer jars or a glove box are highly recommendable methods for processing of subgingival plaque.

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“…Recently there has been an increasing number of studies of anaerobic bacteria and a greater appreciation of their role in the disease process. For example, there is a current awareness that anaerobes are involved in infections in humans much more frequently than had generally been supposed (7). The introduction and development of new techniques have contributed to advances in this area.…”

mentioning

confidence: 99%

Preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria

Chan¹,

deVries²,

Harvey³

1978

J Clin Microbiol

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Several modifications of the roll-tube method have made it simpler for routine use in the isolation and growth of anaerobic bacteria. These include use of a check valve for the production of prereduced anaerobically sterilized media; a Salvarsan tube under oxygen-free gas pressure for the dispensing of molten prereduced anaerobically sterilized agar medium; a Kelly infusion bottle with a graduated pipette side arm (also under gas pressure) for quantitative delivery of fluid prereduced anaerobically sterilized media; and screw-capped prescription bottles for the cultivation of anaerobes. Colonies of Bacteroides melaninogenicus were easily identified and counted by this method. Other anaerobic bacteria have also been cultivated successfully.

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Pathogenic Anaerobic Bacteria

Cited by 10 publications

References 103 publications

Fusobacterium necrophorum infection in mice as a model for the study of liver abscess formation and induction of immunity

Fusobacterium necrophorum infection in mice as a model for the study of liver abscess formation and induction of immunity

Comparison of there anaerobic culture techniques and media for viable recovery of subgingival plaque bacteria

Preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria

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