Pathogenesis of mouse scrapie: Dynamics of agent replication in spleen, spinal cord and brain after infection by different routes

Pathogenesis of Mouse Scrapie: Evidence for Spread of Infection from Central to Peripheral Nervous System

“…), subcutaneous] and relatively low doses of infectivity so that the detection of the onset of replication would not be obscured by any uptake and persistence of the original inoculum. Consistent patterns were obtained showing, successively, replication in spleen and thoracic spinal cord and then, simultaneously, in brain and lumbar cord (Kimberlin & Walker, 1979. In more detailed studies with i.p.…”

Western blot mapping of disease-specific amyloid in various animal species and humans with transmissible spongiform encephalopathies using a high-yield purification method

“…Major contributions to this area have been made by R. H. Kimberlin and C. A. Walker (Kimberlin, 1979;Kimberlin & Walker, 1979. However, for the oral route of infection, the most relevant pathway to natural disease in animal TSE, this aspect has previously received little attention in infectivity studies (Kimberlin & Walker, 1989b) and has not been approached at all with the tools of protein analysis.…”

“…This approach may lead to the localization of the points of entry of the infectious agent into the CNS in experimental as well as in natural disease (Kimberlin & Walker, 1979. Ideally, distinct terminal distribution patterns might even allow a post-mortem identification of the route of infection.…”

Preclinical deposition of pathological prion protein PrPSc in muscles of hamsters orally exposed to scrapie

“…Following up on experimental pathogenesis studies in mice (16)(17)(18)(19) and sheep (20), the animal model used in this study has provided key observations on the spread of infection through the body (4,(21)(22)(23)(24) that have been confirmed in naturally occurring ovine scrapie (25), field cases of BSE (26), orally transmitted BSE in primates (27), and orally transmitted or naturally occurring CWD (28,29). Additionally, more recent reports on the involvement of the sympathetic nervous system in vCJD (30) and the deposition of PrP Sc in muscles of patients with sporadic CJD (14) have shown the relevance of our hamster model for peripheral PrP Sc routing in TSE-affected humans.…”