Pathogenesis of human norovirus genogroup II genotype 4 in post-weaning gnotobiotic pigs

Park, Byung Joo; Jung, Soontag; Choi, Changsun; Myoung, Jinjong; Ahn, Hee-Seop; Han, Sang-Hoon; Kim, Yong Hyun; Go, Hyeon‐Jeong; Lee, Joong‐Bok; Park, Seung‐Yong; Song, Chang‐Seon; Lee, Sang‐Won; Choi, In‐Soo

doi:10.4014/jmb.1810.09061

Cited by 14 publications

(12 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gn piglets develop diarrhea, shed virus, and have detectable levels of HuNoV in the intestines upon oral infection [ 55 , 56 , 57 , 58 , 59 , 60 , 61 ]. Although low, HuNoV-specific serum and mucosal antibodies have been reported in Gn piglets [ 61 ].…”

Section: Large Animal Modelsmentioning

confidence: 99%

Human Norovirus: Experimental Models of Infection

Todd

Tripp

2019

Viruses

View full text Add to dashboard Cite

Human noroviruses (HuNoVs) are a leading cause of acute gastroenteritis worldwide. HuNoV infections lead to substantial societal and economic burdens. There are currently no licensed vaccines or therapeutics for the prevention or treatment of HuNoVs. A lack of well-characterized in vitro and in vivo infection models has limited the development of HuNoV countermeasures. Experimental infection of human volunteers and the use of related viruses such as murine NoV have provided helpful insights into HuNoV biology and vaccine and therapeutic development. There remains a need for robust animal models and reverse genetic systems to further HuNoV research. This review summarizes available HuNoV animal models and reverse genetic systems, while providing insight into their usefulness for vaccine and therapeutic development.

show abstract

Section: Large Animal Modelsmentioning

confidence: 99%

Human Norovirus: Experimental Models of Infection

Todd

Tripp

2019

Viruses

View full text Add to dashboard Cite

show abstract

“…The methods for generating the pcDNA3.1-Neo-JY3 vector and pcDNA3.1-Neo-JY3-3xFLAG-N vector have been previously described [20,27,32]. All constructs utilized in this study were fully accounted in the previous paper [33][34][35]. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to amplify ZIKV genes were previously described in detail [30].…”

Section: Plasmidsmentioning

confidence: 99%

“…Detailed experimental procedures were described elsewhere [33][34][35]. Briefly, HEK293T 8 × 10 5 cells/well were prepared in a 6-well plate.…”

Section: Transfection and Luciferase Assaymentioning

confidence: 99%

Zika Virus-Encoded NS2A and NS4A Strongly Downregulate NF-κB Promoter Activity

Lee

Nguyen

Myoung

2020

J. Microbiol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Since Zika virus (ZIKV) was first detected in Uganda in 1947, serious outbreaks have occurred globally in Yap Island, French Polynesia and Brazil. Even though the number of infections and spread of ZIKV have risen sharply, the pathogenesis and replication mechanisms of ZIKV have not been well studied. ZIKV, a recently highlighted Flavivirus, is a mosquito-borne emerging virus causing microcephaly and the Guillain-Barre syndrome in fetuses and adults, respectively. ZIKV polyprotein consists of three structural proteins named C, prM and E and seven nonstructural proteins named NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5 in an 11-kb single-stranded positive sense RNA genome. The function of individual ZIKV genes on the host innate immune response has barely been studied. In this study, we investigated the modulations of the NF-κB promoter activity induced by the MDA5/RIG-I signaling pathway. According to our results, two nonstructural proteins, NS2A and NS4A, dramatically suppressed the NF-κB promoter activity by inhibiting signaling factors involved in the MDA5/RIG-I signaling pathway. Interestingly, NS2A suppressed all components of MDA5/RIG-I signaling pathway, but NS4A inhibited most signaling molecules, except IKKε and IRF3-5D. In addition, both NS2A and NS4A downregulated MDA5-induced NF-κB promoter activity in a dosedependent manner. Taken together, our results suggest that NS2A and NS4A signifcantly antagonize MDA5/RIG-I-mediated NF-κB production, and these proteins seem to be controlled by different mechanisms. This study could help understand the mechanisms of how ZIKV controls innate immune responses and may also assist in the development of ZIKV-specific therapeutics.

show abstract

“…After culturing competent cells in 4 ml of selective media overnight, plasmid DNA was isolated using FavorPrep Plasmid DNA Extraction Mini Kit (Favorgen, Taiwan) or Dokdo-Prep Plasmid Mini-prep Kit (Elpisbio, Korea). Also, NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) was used to produce a large amount of plasmids [15][16][17].…”

Section: Bacteria Culture and Dna Isolationmentioning

confidence: 99%

Generation of Full-Length Infectious cDNA Clones of Middle East Respiratory Syndrome Coronavirus

Lee¹,

Bae²,

Myoung³

2019

Journal of Microbiology and Biotechnology

Self Cite

View full text Add to dashboard Cite

Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in Saudi Arabia in 2012 and related infection cases have been reported in over 20 countries. Roughly 10,000 human cases have so far been reported in total with fatality rates at up to 40%. The majority of cases have occurred in Saudi Arabia with mostly sporadic outbreaks outside the country except for the one in South Korea in 2015. The Korean MERS-CoV strain was isolated from the second Korean patient and its genome was fully sequenced and deposited. To develop virusspecific protective and therapeutic agents against the Korean isolate and to investigate molecular determinants of virus-host interactions, it is of paramount importance to generate its full-length cDNA. Here we report that two full-length cDNAs from a Korean patientisolated MERS-CoV strain were generated by a combination of conventional cloning techniques and efficient Gibson assembly reactions. The full-length cDNAs were validated by restriction analysis and their sequence was verified by Sanger method. The resulting cDNA was efficiently transcribed in vitro and the T7 promoter-driven expression was robust. The resulting reverse genetic system will add to the published list of MERS-CoV cDNAs and facilitate the development of Korean isolate-specific antiviral measures.

show abstract

Pathogenesis of human norovirus genogroup II genotype 4 in post-weaning gnotobiotic pigs

Cited by 14 publications

References 36 publications

Human Norovirus: Experimental Models of Infection

Human Norovirus: Experimental Models of Infection

Zika Virus-Encoded NS2A and NS4A Strongly Downregulate NF-κB Promoter Activity

Generation of Full-Length Infectious cDNA Clones of Middle East Respiratory Syndrome Coronavirus

Contact Info

Product

Resources

About