Pathogenesis, developmental consequences, and clinical correlations of human embryo fragmentation

Fujimoto, Victor Y.; Browne, Richard W.; Bloom, Michael S.; Sakkas, Denny; Alikani, Mina

doi:10.1016/j.fertnstert.2010.11.033

Cited by 47 publications

(55 citation statements)

References 133 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fertilized zygotes were identified by the appearance of two pronuclei 16-18 h later. Embryo quality was assessed as embryo fragmentation score (EFS) and embryo cell number (ECN) 48 to 72 h after fertilization; higher EFS is generally associated with poorer IVF prognosis [18] and higher ECN is generally associated with a better IVF prognosis [19]. Embryos were transferred on day 2 or 3 post-fertilization based on clinical factors, and a serum hCG test was administered 14 days later to assess implantation.…”

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Ingle

Bloom

Parsons

et al. 2016

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Purpose A hypothesis-generating pilot study exploring associations between essential trace elements measured in follicular fluid (FF) and urine and in vitro fertilization (IVF) endpoints. Methods We recruited 58 women undergoing IVF between 2007 and 2008, and measured cobalt, chromium, copper, manganese, molybdenum, and zinc in FF (n = 46) and urine (n = 45) by inductively coupled plasma mass spectrometry (ICP-MS). We used multivariable regression models to assess the impact of FF and urine trace elements on IVF outcomes, adjusted for age, body mass index, race, and cigarette smoking.Results Trace elements were mostly present at lower concentrations in FF than in urine. The average number of oocytes retrieved was positively associated with higher urine cobalt, chromium, copper, and molybdenum concentrations. FF chromium and manganese were negatively associated with the proportion of mature oocytes, yet urine manganese had a positive association. FF zinc was inversely associated with average oocyte fertilization. Urine trace elements were significant positive predictors for the total number of embryos generated. FF copper predicted lower embryo fragmentation while urine copper was associated with higher embryo cell number and urine manganese with higher embryo fragmentation. No associations were detected for implantation, pregnancy, or live birth. Conclusions Our results suggest the importance of trace elements in both FF and urine for intermediate, although not necessarily clinical, IVF endpoints. The results differed using FF or urine biomarkers of exposure, which may have implications for the design of clinical and epidemiologic investigations. These initial findings will form the basis of a more definitive future study.

show abstract

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Ingle

Bloom

Parsons

et al. 2016

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is well known that embryo fragmentation or the generation of anucleate cell fragments is an important biomarker of embryo quality during early embryonic development after ART [24,25]. Several hypotheses regarding embryo fragmentation have been reported such as programmed cell death (apoptosis) [26], telomere length [27], increased maternal age [28], abnormalities in cytoskeleton and microtubule organization [29,30].…”

Section: Fragmentation (Supplementary Movie 8)mentioning

confidence: 99%

Human Embryonic Behavior Observed with Time-Lapse Cinematography

Mio¹

2014

J Health Med Informat

View full text Add to dashboard Cite

To clarify developmental behavior in human embryos, we observed oocytes with in vitro fertilization and intracytoplasmic sperm injection and donated embryos up to 5 days using our original time-lapse cinematography (TLC) system. The TLC analyses demonstrated several novel aspects and events that were not visible by intermittent stillframe microscopy. Here, we present the entire fertilization process in which we founded the fertilization cone, cytoplasmic flare, dynamic change of nuclear precursor body and cytoplasmic halo. We also demonstrated the occurrence of zygotes with a single pronucleus (PN) and uneven 2PN, fragmentation, blastocoel collapse during the blastocyst stage, and the hatching pattern of expanded blastocysts. Observing such morphokineses in human embryos will enhance our understanding of human embryonic behavior up to preimplantation.

show abstract

“…Embryo cell number (ECN) was assessed on the day of transfer and characterizes cleavage or growth rate; it is a positive predictor for IVF success. Embryo fragmentation score (EFS) was assessed approximately 48 h after fertilization and is an inverse predictor for IVF success [26]. Embryos were transferred on the second or third day post-fertilization.…”

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes

Bloom

Kim

Kruger

et al. 2012

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Purpose We previously reported associations between trace concentrations of Hg, Cd and Pb in blood and urine and reproductive outcomes for women undergoing in-vitro fertilization (IVF). Here we assess measurements in single follicular fluid (FF) specimens from 46 women as a presumably more relevant marker of dose for reproductive toxicity. Methods FF specimens were analyzed for Hg, Cd and Pb using sector field-inductively coupled plasma-mass spectrometry (SF-ICP-MS). Variability sources were assessed by nested ANOVA. Multivariable regression was used to evaluate associations for square root transformed metals with IVF outcomes, adjusting for confounders.

show abstract

Pathogenesis, developmental consequences, and clinical correlations of human embryo fragmentation

Cited by 47 publications

References 133 publications

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Human Embryonic Behavior Observed with Time-Lapse Cinematography

Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes

Contact Info

Product

Resources

About