Pathogenesis and Virulence of Mycoplasma bovis

Perez-Casal, José

doi:10.1016/j.cvfa.2020.02.002

Cited by 29 publications

(17 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secreted proteins of mycoplasmas might function as virulence-related factors, it is difficult to confirm them due to the limited and inefficient genetic tools and cell/animal models [5]. We previously identified MbovP0145 as a unique secreted protein of M. bovis with Sec/SPII signal peptides and determined that it is worth to investigate further, because it has two DUF285 domains and BspA, the family of LRR surface proteins.…”

Section: Discussionmentioning

confidence: 99%

“…The pathogenesis of M. bovis can be attributed to a variety of membrane proteins related to virulence. These include adhesins, a family of variable surface proteins (VSPs) modifying as antigen diversity; proteins with nuclease activities [5], IgG-binding/cleavage abilities [6], and the ability to form biofilms [7]; and cytoplasm proteins, such as MbovP327 and MbovP328, with phosphodiesterase abilities to degrade cyclic dinucleotides and/or nanoRNAs, which is essential to M. bovis growth under cocultures with host cells [8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Secreted MbovP0145 Promotes IL-8 Expression through Its Interactive β-Actin and MAPK Activation and Contributes to Neutrophil Migration

Zhang

et al. 2021

Pathogens

View full text Add to dashboard Cite

Mycoplasma bovis (M. bovis) is an important pathogen of cattle responsible for huge economic losses in the dairy and beef industries worldwide. The proteins secreted by M. bovis are mainly related to its adhesion, invasion, virulence, and intracellular survival and play a role in mycoplasma–host interactions. In our previous study, we found MbovP0145, a secreted protein present in the M. bovis secretome, but little is known about its function. In this study, we assessed the inflammatory characteristics and underlined mechanism of this inflammation of recombinant MbovP0145 (rMbovP0145). For this, bovine lung epithelial cells (EBL) were stimulated by rMbovP0145 to see the IL-8 production in a time- and dose-dependent manner. We observed that rMbovP0145 increased the production of IL-8 via ERK1/2 and P38 pathway activation. Further, the effect of the M. bovis ΔMbov_0145 mutant and its complementary strain on IL-8 mRNA expression was also confirmed. A pulldown assay of the GST-tagged MbovP0145 protein with mass spectrometry demonstrated that β-actin could specifically interact with rMbovP0145 to mediate the IL-8 signaling. As knockdown of β-actin expression with RNA interference in EBL cells decreased the mRNA expression of IL-8 and the phosphorylated ERK1/2 and P38 proteins, whereas disrupted actin polymerization by cytochalasin D led to a significantly higher IL-8 expression and MAPK phosphorylation in rMbovP0145-stimulated cells. Compared to M. bovis HB0801 and its complementary strain, the culture supernatant of EBL cells infected with the M. bovis ΔMbov_0145 mutant induced less neutrophil migration to the lower chamber in a transwell system. In conclusion, MbovP0145 promoted IL-8 expression by interacting with β-actin through activation of the MAPK pathway, thus contributing to neutrophil migration.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Secreted MbovP0145 Promotes IL-8 Expression through Its Interactive β-Actin and MAPK Activation and Contributes to Neutrophil Migration

Zhang

et al. 2021

Pathogens

View full text Add to dashboard Cite

show abstract

“…These viruses are generally primary obligate pathogens, and most of them facilitate superinfection by bacterial pathogens such as Pasteurella multocida, Mannheimia haemolytica, Histophilus somni, Trueperella pyogenes , and Mycoplasma (M.) bovis ( 6 ). M. bovis is an important cause of BRD, particularly the form that manifests as chronic pneumonia ( 7 , 8 ). The economic impact of M. bovis can be severe.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Mycoplasma bovis in Algeria and Characterisation of the Isolated Clones

et al. 2022

View full text Add to dashboard Cite

Bovine respiratory disease (BRD) is common in calves in Algeria, but to date, Mycoplasma bovis has never been monitored as a potential etiological agent. Here, to assess the presence (direct detection) and circulation (indirect detection) of M. bovis, broncho-alveolar lavage fluids (BALF) and serum samples were collected from 60 veal calf farms in Algeria. A commercial ELISA kit (ID Screen® ELISA) was used to screen for the presence of specific antibodies against M. bovis in 351 blood sera collected from both diseased and healthy calves, and 69% (241 sera) tested positive. BALFs from the 176 diseased calves were used to screen for M. bovis by real-time-PCR (rt-PCR), and 102 (58%) tested positive. A non-exhaustive set of 53 clones were isolated from 44 calves and further subtyped using polC gene sequencing. No predominant subtype was found, and two clones exhibited a new subtype. Fourteen clones were further characterized by multilocus sequence typing, and results showed a high degree of genetic diversity, with some clones having new alleles and subtypes. The minimum inhibitory concentrations (MICs) of 5 antimicrobials regularly used to treat BRD was determined on 45 clones. Susceptibility profiles showed very broad diversity, confirming the variety of clones actively circulating. We detected clones with high MICs, including increased MICs of enrofloxacin (n = 5). This is the first study to report the presence of M. bovis in Algeria in calves with BRD. This research also finds broad genetic and phenotypic diversity in the actively circulating isolates.

show abstract

“…For these reasons M. dispar is included with M. bovis amongst the agents that cause or exacerbate BRD [5,6]. However, unlike M. bovis which has been thoroughly studied during the past years [17,18], M. dispar has been neglected partly because it is extremely fastidious to culture and requires a specific medium to enable growth. Moreover, its colonies do not show-especially during the early passages-the typical "fried-egg" appearance.…”

Section: Introductionmentioning

confidence: 99%

The pathogen Mycoplasma dispar Shows High Minimum Inhibitory Concentrations for Antimicrobials Commonly Used for Bovine Respiratory Disease

et al. 2020

View full text Add to dashboard Cite

Mycoplasma dispar is an overlooked pathogen often involved in bovine respiratory disease (BRD), which affects cattle around the world. BRD results in lost production and high treatment and prevention costs. Additionally, chronic therapies with multiple antimicrobials may lead to antimicrobial resistance. Data on antimicrobial susceptibility to M. dispar is limited so minimum inhibitory concentrations (MIC) of a range of antimicrobials routinely used in BRD were evaluated using a broth microdilution technique for 41 M. dispar isolates collected in Italy between 2011–2019. While all isolates had low MIC values for florfenicol (<1 μg/mL), many showed high MIC values for erythromycin (MIC90 ≥8 μg/mL). Tilmicosin MIC values were higher (MIC50 = 32 μg/mL) than those for tylosin (MIC50 = 0.25 μg/mL). Seven isolates had high MIC values for lincomycin, tilmicosin and tylosin (≥32 μg/mL). More, alarmingly, results showed more than half the strains had high MICs for enrofloxacin, a member of the fluoroquinolone class considered critically important in human health. A time-dependent progressive drift of enrofloxacin MICs towards high-concentration values was observed, indicative of an on-going selection process among the isolates.

show abstract

Pathogenesis and Virulence of Mycoplasma bovis

Cited by 29 publications

References 51 publications

Secreted MbovP0145 Promotes IL-8 Expression through Its Interactive β-Actin and MAPK Activation and Contributes to Neutrophil Migration

Secreted MbovP0145 Promotes IL-8 Expression through Its Interactive β-Actin and MAPK Activation and Contributes to Neutrophil Migration

Prevalence of Mycoplasma bovis in Algeria and Characterisation of the Isolated Clones

The pathogen Mycoplasma dispar Shows High Minimum Inhibitory Concentrations for Antimicrobials Commonly Used for Bovine Respiratory Disease

Contact Info

Product

Resources

About