Pathogenesis and Management of Bacterial Biofilms in the Urinary Tract

Kumoh, Hiromi

doi:10.1007/bf02355193

Cited by 22 publications

(19 citation statements)

References 39 publications

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“…However, treatment with DNase seemed to reduce the thickness of the wires but did not remove them, as seen by CLSM, similar to findings by Alhede et al (2). Krautgartner et al (29) showed that fibrin and NETs cannot be discriminated by SEM based on morphological criteria but that CLSM can be used to visualize fully hydrated bacterial biofilms (30), and thereby DNA wires. The origin of the DNA was not determined, and further studies are required to determine whether it arises from bacteria or PMNs.…”

Section: Discussionsupporting

confidence: 71%

Interactions between Polymorphonuclear Leukocytes and Pseudomonas aeruginosa Biofilms on Silicone Implants In Vivo

et al. 2012

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dChronic infections with Pseudomonas aeruginosa persist because the bacterium forms biofilms that are tolerant to antibiotic treatment and the host immune response. Scanning electron microscopy and confocal laser scanning microscopy were used to visualize biofilm development in vivo following intraperitoneal inoculation of mice with bacteria growing on hollow silicone tubes, as well as to examine the interaction between these bacteria and the host innate immune response. Wild-type P. aeruginosa developed biofilms within 1 day that trapped and caused visible cavities in polymorphonuclear leukocytes (PMNs). In contrast, the number of cells of a P. aeruginosa rhlA mutant that cannot produce rhamnolipids was significantly reduced on the implants by day 1, and the bacteria were actively phagocytosed by infiltrating PMNs. In addition, we identified extracellular wire-like structures around the bacteria and PMNs, which we found to consist of DNA and other polymers. Here we present a novel method to study a pathogen-host interaction in detail. The data presented provide the first direct, high-resolution visualization of the failure of PMNs to protect against bacterial biofilms.

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Section: Discussionsupporting

confidence: 71%

Interactions between Polymorphonuclear Leukocytes and Pseudomonas aeruginosa Biofilms on Silicone Implants In Vivo

et al. 2012

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“…In this model, the bacteria enmeshed in biofilms formed on the surface of the PT in the bladder play an important role in the persistence of the infection in the bladder and kidneys (13). The antibacterial activity against the bacteria in a biofilm mode of growth might have clinical relevance (20), especially for urinary tract infections associated with foreign bodies, including urinary catheters and stents (12).…”

Section: Vol 47 2003 Antibacterial Activities Of Dk-507k 3755mentioning

confidence: 99%

InVitro and In Vivo Antibacterial Activities of DK-507k, aNovelFluoroquinolone

Otani

Tanaka

Ito

et al. 2003

Antimicrob Agents Chemother

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The antibacterial activities of DK-507k, a novel quinolone, were compared with those of other quinolones: ciprofloxacin, gatifloxacin, levofloxacin, moxifloxacin, sitafloxacin, and garenoxacin (BMS284756). DK-507k was as active as sitafloxacin and was as active as or up to eightfold more active than gatifloxacin, moxifloxacin, and garenoxacin against Streptococcus pneumoniae, methicillin-susceptible and methicillin-resistant Staphylococcus aureus, and coagulase-negative staphylococci. DK-507k was as active as or 4-fold more active than garenoxacin and 2-to 16-fold more active than gatifloxacin and moxifloxacin against ciprofloxacin-resistant strains of S. pneumoniae, including clinical isolates and in vitro-selected mutants with known mutations. DK-507k inhibited all ciprofloxacin-resistant strains of S. pneumoniae at 1 g/ml. A time-kill assay with S. pneumoniae showed that DK-507k was more bactericidal than gatifloxacin and moxifloxacin. The activities of DK-507k against most members of the family Enterobacteriaceae were comparable to those of ciprofloxacin and equal to or up to 32-fold higher than those of gatifloxacin, levofloxacin, moxifloxacin, and garenoxacin. DK-507k was fourfold less active than sitafloxacin and ciprofloxacin against Pseudomonas aeruginosa, while it was two to four times more potent than levofloxacin, gatifloxacin, moxifloxacin, and garenoxacin against P. aeruginosa. In vivo, intravenous treatment with DK-507k was more effective than that with gatifloxacin and moxifloxacin against systemic infections caused by S. aureus, S. pneumoniae, and P. aeruginosa in mice. In a mouse model of pneumonia due to penicillin-resistant S. pneumoniae, DK-507k administered subcutaneously showed dose-dependent efficacy and eliminated the bacteria from the lungs, whereas gatifloxacin and moxifloxacin had no significant efficacy. Oral treatment with DK-507k was slightly more effective than that with ciprofloxacin in a rat model of foreign body-associated urinary tract infection caused by a P. aeruginosa isolate for which the MIC of DK-507k was fourfold higher than that of ciprofloxacin. Oral administration of DK-507k to rats achieved higher peak concentrations in serum and higher concentrations in cumulative urine than those achieved with ciprofloxacin. These data indicate the potential advantages of DK-507k over other quinolones for the treatment of a wide range of community-acquired infections.

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“…Typically, the biofilm mode of bacterial growth on the surface of the urinary catheter and adjacent mucosa accounts for the pathophysiology of foreign body-associated UTI (6)(7)(8). The role of bacterial biofilms on the surface of urinary catheters in UTI has been studied in rabbit models (5,13).…”

Section: Abstract: Rat Model Pseudomonas Aeruginosa Urinary Tract mentioning

confidence: 99%

A Non‐Surgical Rat Model of Foreign Body‐Associated Urinary Tract Infection with Pseudomonas aeruginosa

Kurosaka

Ishida

Yamamura

et al. 2001

Microbiology and Immunology

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This study established a rat model of foreign body-associated urinary tract infection. A spiral polyethylene tube (PT) was placed transurethrally into the bladder without surgical manipulation, followed by transurethral inoculation with Pseudomonas aeruginosa. The persistence of P. aeruginosa in the kidneys and bladder was significantly enhanced by placement of the PT, whereas the bacteria were eliminated rapidly from the urinary tract in the animals without the PT. Scanning electron microscopy revealed a thick biofilm on the surface of the PT from the early stage of infection. Histopathologically, acute pyelonephritis was followed by chronic renal inflammation as well as continuous and sporadic polymorphonuclear leukocyte accumulation and hemorrhage in the pelvis and adjacent tissues, suggesting continuous ascending introduction of the bacteria from the biofilm adhering to the PT. We believe our model simulates the pathophysiology of foreign body-associated urinary tract infection characterized by biofilm formation on the surface of a foreign body.Key words: Rat model, Pseudomonas aeruginosa, Urinary tract infection, Foreign body-associated UTI Urinary tract infections (UTI) associated with foreign bodies including urinary catheters and/or stents are some of the most common and problematic of hospitalacquired infections (3, 16). Typically, the biofilm mode of bacterial growth on the surface of the urinary catheter and adjacent mucosa accounts for the pathophysiology of foreign body-associated UTI (6-8). The role of bacterial biofilms on the surface of urinary catheters in UTI has been studied in rabbit models (5, 13). To determine the pathogenesis of foreign body-associated UTI in terms of its immunological and bacteriological aspects, numerous efforts have been made to establish rodent models of foreign body-associated UTI. These include models in which UTI was induced by placement of foreign bodies, such as glass beads (I), zinc rings (10, 12, 15), catheter segments (4), sutures (11), and polyurethane sponges, into the bladder (9). These models, however, have had few clinical parallels mainly because surgical manipulation to implant the foreign body into the bladder could not be avoided. Surgical incision of the bladder

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Pathogenesis and Management of Bacterial Biofilms in the Urinary Tract

Cited by 22 publications

References 39 publications

Interactions between Polymorphonuclear Leukocytes and Pseudomonas aeruginosa Biofilms on Silicone Implants In Vivo

Interactions between Polymorphonuclear Leukocytes and Pseudomonas aeruginosa Biofilms on Silicone Implants In Vivo

InVitro and In Vivo Antibacterial Activities of DK-507k, aNovelFluoroquinolone

A Non‐Surgical Rat Model of Foreign Body‐Associated Urinary Tract Infection with Pseudomonas aeruginosa

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