Pathogen specific T-lymphocytes for the reconstitution of the immunocompromised host

Pira, Giuseppina Li; Kapp, Markus; Manca, Fabrizio; Einsele, Hermann

doi:10.1016/j.coi.2009.08.006

Cited by 13 publications

(5 citation statements)

References 64 publications

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“…The second one is analysis of specific cellular immunity, that is, testing T-cell responses towards a limited array of antigen preparations (proteins or peptide libraries) using PBMC obtained from small blood samples. It should be noted that many of the assays described here can also be scaled up for preparative purposes to purify virus specific T cells for adoptive immunoreconstitution, as recently reviewed [112]. In conclusion, T-cell epitope mapping is relevant for vaccine development, for dissection of the quality of response in vaccinees or in infected subjects and for definition of peptide libraries as screening tools of cellular immunity.…”

Section: Discussionmentioning

confidence: 97%

High Throughput T Epitope Mapping and Vaccine Development

Pira¹,

Ivaldi

Moretti

et al. 2010

Journal of Biomedicine and Biotechnology

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Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development. In fact, mapping of T-cell epitopes provides useful information for the design of peptide-based vaccines and of peptide libraries to monitor specific cellular immunity in protected individuals, patients and vaccinees. Nevertheless, epitope mapping is a challenging task. In fact, large panels of overlapping peptides need to be tested with lymphocytes to identify the sequences that induce a T-cell response. Since numerous peptide panels from antigenic proteins are to be screened, lymphocytes available from human subjects are a limiting factor. To overcome this limitation, high throughput (HTP) approaches based on miniaturization and automation of T-cell assays are needed. Here we consider the most recent applications of the HTP approach to T epitope mapping. The alternative or complementary use of in silico prediction and experimental epitope definition is discussed in the context of the recent literature. The currently used methods are described with special reference to the possibility of applying the HTP concept to make epitope mapping an easier procedure in terms of time, workload, reagents, cells and overall cost.

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Section: Discussionmentioning

confidence: 97%

High Throughput T Epitope Mapping and Vaccine Development

Pira¹,

Ivaldi

Moretti

et al. 2010

Journal of Biomedicine and Biotechnology

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show abstract

“…The ELISPOT assay has similar advantages, but it measures the cytokine producing function without appreciating the proliferative capacity of specific T-cells (Kern et al, 2005). However, assessment of antigen-specific immunity is often limited by the number of available PBMC, due to small size of the blood sample or to a lymphopenic status of the patient (Li Pira et al, 2007, 2009.…”

Section: Discussionmentioning

confidence: 99%

Lymphocyte proliferation specific for recall, CMV and HIV antigens in miniaturized and automated format

Pira

Starc

Conforti

et al. 2012

Journal of Immunological Methods

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“…The number of CD3 + T cells may be a valuable tool both to identify patients at high risk of developing HAdV infection and to predict the probability of overcoming viral infection . Theoretically, the adoptive transfer of pathogen‐specific T‐cell immunity from the HSCT donor has curative potential for patients with HAdV‐related complications . Studies on the infusion of in vitro ‐expanded, pathogen‐specific T cells have provided convincing proof‐of‐principle in favor of this approach for CMV, EBV, and HAdV infections .…”

Section: Brief Reportmentioning

confidence: 99%

Adoptive immunotherapy with antigen-specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus-related respiratory failure in a child given haploidentical stem cell transplantation

Nardo

Pira

Amodeo

et al. 2013

Pediatr Blood Cancer

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We report on the successful infusion of human adenovirus (HAdV)-specific T cells in a child with congenital amegakaryocytic thrombocytopenia, given T-cell-depleted hematopoietic stem cell transplantation (HSCT) from the HLA-haploidentical mother during extracorporeal membrane oxygenation (ECMO) for severe HAdV-related respiratory failure. Donor-derived, interferon (IFN)-γ-secreting HAdV-specific T cells were enriched using the cytokine capture assay, after in vitro stimulation with overlapping peptides from the immunodominant HAdV5 hexon protein. Two weeks after T-cell transfer, viral load decreased and ECMO was discontinued. T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration.

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Pathogen specific T-lymphocytes for the reconstitution of the immunocompromised host

Cited by 13 publications

References 64 publications

High Throughput T Epitope Mapping and Vaccine Development

High Throughput T Epitope Mapping and Vaccine Development

Lymphocyte proliferation specific for recall, CMV and HIV antigens in miniaturized and automated format

Adoptive immunotherapy with antigen-specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus-related respiratory failure in a child given haploidentical stem cell transplantation

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