Pathogen resistance and gene frequency stability of major histocompatibility complex class IIB alleles in the giant spiny frog Quasipaa spinosa

Hu, Wenfang; Dong, Baojuan; Kong, Shenshen; Mao, Yuanyuan; Zheng, Rongquan

doi:10.1016/j.aquaculture.2016.11.001

Cited by 8 publications

(3 citation statements)

References 51 publications

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“…The giant spiny frog Quasipaa spinosa (David 1875) is distributed in China and Vietnam, particularly in southern and southeast parts of China (Hu et al, 2017). Owing to its narrow optimum temperature range (20-24°C), it could be a suitable heterothermic animal model to assess the impact of thermal stress, including cold and heat stress, on amphibians (Shu et al, 2017;Xie et al, 2017;Zheng and Liu, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Liu

et al. 2018

Journal of Experimental Biology

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Ambient temperature-associated stress can affect normal physiological functions in ectotherms. To assess the effects of cold or heat stress on amphibians, giant spiny frogs () were acclimated at 22°C followed by exposure to 5°C or 30°C for 0, 3, 6, 12, 24 and 48 h, respectively. Histological alterations, apoptotic index, generation of mitochondrial reactive oxygen species (ROS), antioxidant activity indices and stress-response gene expression in frog livers were subsequently determined. Results showed that many fat droplets appeared after 12 h of heat stress and the percentage of melanomacrophage centres significantly changed after 48 h at both stress conditions. Furthermore, the mitochondrial ROS levels were elevated in a time-dependent manner up to 6 h and 12 h in the cold and heat stress groups, respectively. The activities of superoxide dismutase, glutathione peroxidase and catalase were successively increased with increasing periods of cold or heat exposure, and their gene expression levels showed similar changes in both stress conditions. Most tested heat shock protein (HSP) genes were sensitive to temperature exposure, and the expression profiles of most apoptosis-related genes was significantly upregulated at 3 and 48 h under cold and heat stress, respectively. Apoptotic index at 48 h under cold stress was significantly higher than that under heat stress. Notably, lipid droplets, , and might be suitable bioindicators of heat stress. The results of these alterations at physiological, biochemical and molecular levels might contribute to a better understanding of the stress response of, and perhaps amphibians more generally, under thermal stress.

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Section: Introductionmentioning

confidence: 99%

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Liu

et al. 2018

Journal of Experimental Biology

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“…However, under intensive high-density culture, Rana catesbeianus has a slow growth rate, low feed utilization rate, and weak resistance to bacteria and viruses. Rana catesbeianus is prone to bacterial or fungal diseases such as red-leg syndrome and chytridiomycosis [ 14 , 15 ]. On account of the pursuit of economic gains, some breeders utilize an excessive amount of prohibited and restricted veterinary drugs during animal feeding and veterinary treatment, which seriously affects the healthy development of the industry and raises social concerns about food safety [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on Broad-Spectrum Antibody for Simultaneous Determination of Thirteen Fluoroquinolone Antibiotics in Rana catesbeianus

Zhang

Lang

Guo

et al. 2023

Foods

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Fluoroquinolone (FQ) is a type of widely used antibiotic in agriculture and aquaculture, and exposure to low doses of FQs may result in the transfer of resistance between animal and human pathogens. Based on the optimization of the operating parameters, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) standard curve was constructed for the simultaneous detection of 13 FQs, including enrofloxacin (ENR), ciprofloxacin (CIP), sarafloxacin (SAR), ofloxacin (OFL), norfloxacin (NOR), pefloxacin mesylate (PM), pefloxacin (PEF), enoxacin (ENX), marbofloxacin (MAR), fleroxacin (FLE), lomefloxacin (LOM), danofloxacin (DAN), and difloxacin (DIF). The limit of detection (LOD, computed as IC10) and sensitivity (IC50) of the ic-ELISA for ENR were 0.59 μg/L and 19.23 μg/L, respectively. The precision and dependability of the detection results of this ic-ELISA were properly verified by HPLC in Rana catesbeianus samples. This indicated that the established ic-ELISA approach could be utilized to determine the FQs in Rana catesbeianus. In addition, this ic-ELISA, based on a broad-spectrum antibody, provides a technical reference and potential strategy for an immunoassay of hazard factors with similar structure.

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“…The genus has no established common name, but many individual species are referred to as spiny frogs. They consist of 11 species distributed in east and southeast Asia, from Thailand and Cambodia to southern and eastern China (Frost, 2014;Hu, Dong, Kong, Mao, & Zheng, 2017;Kong, Zheng, & Zhang, 2016). Most species of Quasipaa inhabit rocky streams and mountains from 300 to 1,900 m above sea level (Fei, 2012), and they are likely to possess high genetic divergences between populations (Che et al, 2009).…”

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confidence: 99%

Interspecies introgressive hybridization in spiny frogsQuasipaa(Family Dicroglossidae) revealed by analyses on multiple mitochondrial and nuclear genes

Zhang

Zhou

et al. 2017

Ecology and Evolution

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Introgression may lead to discordant patterns of variation among loci and traits. For example, previous phylogeographic studies on the genus Quasipaa detected signs of genetic introgression from genetically and morphologically divergent Quasipaa shini or Quasipaa spinosa. In this study, we used mitochondrial and nuclear DNA sequence data to verify the widespread introgressive hybridization in the closely related species of the genus Quasipaa, evaluate the level of genetic diversity, and reveal the formation mechanism of introgressive hybridization. In Longsheng, Guangxi Province, signs of asymmetrical nuclear introgression were detected between Quasipaa boulengeri and Q. shini. Unidirectional mitochondrial introgression was revealed from Q. spinosa to Q. shini. By contrast, bidirectional mitochondrial gene introgression was detected between Q. spinosa and Q. shini in Lushan, Jiangxi Province. Our study also detected ancient hybridizations between a female Q. spinosa and a male Q. jiulongensis in Zhejiang Province. Analyses on mitochondrial and nuclear genes verified three candidate cryptic species in Q. spinosa, and a cryptic species may also exist in Q. boulengeri. However, no evidence of introgressive hybridization was found between Q. spinosa and Q. boulengeri. Quasipaa exilispinosa from all the sampling localities appeared to be deeply divergent from other communities. Our results suggest widespread introgressive hybridization in closely related species of Quasipaa and provide a fundamental basis for illumination of the forming mechanism of introgressive hybridization, classification of species, and biodiversity assessment in Quasipaa.

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Pathogen resistance and gene frequency stability of major histocompatibility complex class IIB alleles in the giant spiny frog Quasipaa spinosa

Cited by 8 publications

References 51 publications

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on Broad-Spectrum Antibody for Simultaneous Determination of Thirteen Fluoroquinolone Antibiotics in Rana catesbeianus

Interspecies introgressive hybridization in spiny frogsQuasipaa(Family Dicroglossidae) revealed by analyses on multiple mitochondrial and nuclear genes

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