Pathogen isolation and antibiogram analysis in dairy cows with clinical mastitis in Adana region, Turkey

Abstract

The goal of this study was to investigate pathogen types found in milk samples from cows with clinical mastitis, as well as the sensitivity of isolated bacteria to different antibiotics. The study was carried out on 103 milk samples collected from dairy cows with clinical mastitis. Microbiological isolation and identification were performed to identify gram negative and gram positive bacteria, Mycoplasma bovis and fungi. Then, bacteria isolated from infected milks were subjected to antibiogram, using 27 antimicrobials agents. As a result, a total of 146 isolates were obtained from 103 milk samples. The predominant pathogen isolates recovered were fungi (21.9%), Escherichia coli (19.9%) and Staphylococcus aureus (13.7%), followed by Mycoplasma bovis (8.2%) and Streptococcus uberis (6.8%). The mixed predominant pathogen was fungi, (75%) the majority of which mixed with gram positive bacteria. Ampicillin, Imipenem, Chloramphenicol and Streptomycin were the least effective antimicrobial agents, while the most effective agents were Florfenicol, followed by Amikacin and Kanamycin / Cefalexin. In conclusion, the types of microorganisms that cause mastitis and the antibiogram results of the bacteria isolated varied. Above all, fungal mastitis is a severe problem that should always be considered prior to starting antibiotic treatment.

HMOX1 Promotes Ferroptosis in Mammary Epithelial Cells via FTH1 and Is Involved in the Development of Clinical Mastitis in Dairy Cows

“…Holstein cows (6 years of age) from a commercial farm (Wuzhong City, Ningxia Hui Autonomous Region, China) were evaluated for clinical mastitis. The udder examination was performed according to the criteria for veterinary clinical diagnosis, including redness, temperature, swelling and pain [ 20 ]. Milk samples (20–25 mL) from the Holstein cows were collected for somatic cell count (SCC) as described previously [ 21 ].…”

Histopathological evaluation of uterine structure and immunohistochemical examination of MMP-1 and TIMP-1 in cows recovered from metritis

“…In addition, the cows in both groups were between about 3.5-5 years old, and did not have any clinical problems at the time of study. The previous health and disease information of the cows was obtained from the records, and metritis was characterized as previously described, taking into account findings such as fever, and fetid watery red-brown uterine discharge (Genís et al, 2018;Kurt et al, 2019). In addition, animals in two groups were included in the study according to the results of microbiological examination.…”

“…Uterine infections have an incidence of up to 50% in the postpartum period in dairy cows (Manimaran et al, 2016). It has also been reported that the most common uterine disease is metritis in dairy cows (Kurt et al, 2019), with an approximately 20% incidence (Pérez-Báez et al, 2021). Moreover, uterine diseases can occur in the form of endometritis or metritis.…”

“…Metritis is defined as inflammation of the uterine wall caused by various pathogens and is a disease characterized by systemic findings such as fever, watery redbrown and foul-smelling uterine discharge, loss of appetite, and high heart rate (Genís et al, 2018). Metritis result in negative outcomes such as prolongation of the calving to conception interval, delayed resumption of ovarian cyclicity, decrease in conception rates, and milk yield and increase in culling rate in farm animals (Manimaran et al, 2016;Kurt et al, 2019;Pérez-Báez et al, 2021). Because uterine infections directly disrupt the uterine environment (Sheldon et al, 2006) within connective tissues and endometrial cells, histological recovery of the uterus can take time.…”

DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis

“…Mastitis in cows is categorized as a subclinical and clinical inflammatory disease of the mammary gland caused by a variety of stimulating factors, such as pathogens, physical and chemical factors and mechanical stimulus. It negatively affects animal welfare, milk quality, farmer serenity, and farming profitability and causes substantial economic losses throughout the world ( 1 , 2 ). Previous researches have included investigation into the prevention and treatment of cow mastitis, such as vaccine development ( 2 , 3 ), discovery of new drugs and new approaches of treatment ( 4 ), and exploring the veterinary medicinal compounds and monomer extracts of traditional Chinese medicine ( 5 ).…”