Pathogen Identification of Clubroot Disease in Chinese Cabbage from Yuanyang County, Henan Province

Abstract

To clarify the causal agent of clubroot disease in Chinese cabbage from Yuanyang experimental base, the isolate YY was collected from the clubroot for further research. After morphological, cytological observation and pathogenicity identification, the pathogen was preliminary inferred as Plasmodiophora brassicae. In order to quickly detect the pathogen, specific primers were designed according to D85819 and Pro1 gene, respectively, and used to amplify the expected fragment from the template DNA of target pathogen. After PCR and sequence analysis, they showed 99% and 93% identity with the D85819 and Pro1 gene sequences of P. brassicae from NCBI respectively. These results indicated that the pathogen was finally confirmed as P. brassicae, which were coincident with preliminary speculation. Moreover, Pro1-specific marker developed in this study can be applied to quick and accurate detection of P. brassicae isolate, which appears a useful assay method for detecting P. brassicae resting spores and improves efforts to halt the dissemination and better control this pathogen.

Germplasm Enhancement and Identification of Loci Conferring Resistance against Plasmodiophora brassicae in Broccoli

“…Root gall samples from Chinese cabbage plants were collected from Xinye, Henan province, and the pathogen was identified and reported as race 4 [ 20 , 21 , 22 ]. Inoculation and resistance testing were performed using our previously described method [ 23 ].…”

Population genomics of the rice landrace Acuce reveals exceptional dynamics of immune receptors

“…The Yuanyang terraces (YYT; Yunnan, China) were shaped by the agricultural practices of the Hani ethnic group [7]; they were classified by UNESCO in 2013 as a Cultural Landscape and are known for being home to a wide range of rice landraces [8]. Quite importantly, the available records indicate that the YYT only suffer 1% losses caused by disease [9], a striking feature compared to the well-documented 32% losses at the global level [10]. High genetic diversity of some YYT landraces has been documented in previous reports using a limited number of molecular markers [11][12][13], suggesting they resemble composite populations.…”

Race- and Isolate-specific Molecular Marker Development through Genome-Realignment Enables Detection of Korean Plasmodiophora brassicae Isolates, Causal agents of Clubroot Disease

“…Development of race-specific markers would enable more effective and precise detection of this pathogen. Such molecular markers have been used effectively in plant disease diagnostics for many years, including limited methods in P. brassicae based on PCR amplification of DNA from galls or resting spores ( Faggian and Strelkov, 2009 ; Ito et al, 1997 , 2008 ; Li et al, 2017 ; Wallenhammar and Arwidsson, 2001 ; Yang et al, 2002 ). Ito et al (1997) cloned and sequenced 1.4 kb DNA fragment from 19 different Japanese P. brassicae isolates and found that the markers covering that sequence do not amplify fungi and bacterial DNA they studied.…”

“…The authors, however, found that isolates from two different countries had variation in 18s and ITS1 (inter-transcribed spacer 1) regions indicating that those markers are unable to differentiate any isolate specific variations. In a recent study in China, Li et al (2017) successfully detected an unknown P. brassicae isolate after targeting D85819 and Pro1 gene-specific primers. However, none of those PCR based methods could distinguish between the different P. brassicae isolates.…”