Pathogen detection using single mode planar optical waveguides

Martinez, Jennifer S.; Grace, W. Kevin; Grace, Karen M.; Hartman, N. F.; Swanson, Basil I.

doi:10.1039/b502329g

Cited by 41 publications

(77 citation statements)

References 25 publications

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“…We have successfully demonstrated the application of the waveguide biosensor to the rapid and sensitive detection of nanomolar quantities of B. anthracis and breast cancer proteins in complex samples. 14 …”

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confidence: 99%

Detection of Intact Influenza Viruses using Biotinylated Biantennary S-Sialosides

et al. 2008

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confidence: 99%

Detection of Intact Influenza Viruses using Biotinylated Biantennary S-Sialosides

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“…7 The planar silicon oxynitride (SiONx) optical waveguides are fabricated at nGimat Ltd (Atlanta) and are coated with a 10-nm surface of SiO2 to enable functionalization at Spectrum Thin Films Inc. Details of these waveguides are described elsewhere.…”

Section: Waveguide-based Optical Biosensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…[7][8][9][10][11] This biosensor relies on evanescent sensing on a planar waveguide surface to amplify the transduction signal of the fluorescently labeled antibodies to the target antigen. 7,12 We have also developed novel surface functionalization chemistries and target pull-down strategies to increase the sensitivity and specificity of our assays. 10,[13][14][15][16] Specifically, our work has targeted the detection of pathogen-specific biomarkers with exquisite sensitivity and specificity, with minimal sample processing, so as to facilitate the rapid point-of-care diagnosis of infection.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis

Sakamuri

Waters

et al. 2017

ANAL. SCI.

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Early and rapid detection of bovine tuberculosis (bTB) is critical to controlling the spread of this disease in cattle and other animals. In this study, we demonstrate the development of an immunoassay for the direct detection of the bovine bTB biomarker, lipomannan (LM) in serum using a waveguide-based optical biosensor. We apply an ultra-sensitive detection strategy developed by our team, termed lipoprotein capture, that exploits the pull-down of high-density lipoprotein (HDL) nanodiscs from cattle blood that allows for the recovery and detection of associated LM. We also profile the change in the expression of these TB biomarkers as a function of time from a small set of samples collected from studies of bovine TB-infected cattle. We demonstrate for the first time the direct detection of bovine LM in serum, and clearly show that the biomarker is expressed in detectable concentrations during the entire course of the infection.

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“…Measurements are extremely rapid (3 sec), yield minimal non-specific and background signals, and can be adapted to the simultaneous detection of a wide variety of fluorescent labels. This biosensor has been previously validated for the detection of anthrax [3], influenza [4], breast cancer [5] and tuberculosis [6][7][8]. Here we have extended the utility of this platform to detect Gram-positive pathogens, which include the clinically relevant Staphylococcus spp.…”

Section: Introductionmentioning

confidence: 99%

Membrane Insertion for Direct Detection of Lipoteichoic Acid

Kubicek-Sutherland

Hengartner

Mukundan

2017

Trans. Mat. Res. Soc. Japan

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The incorrect diagnosis of bacterial pathogens results in the misuse of antibiotics, a major contributor to the evolution of antibiotic resistance. The ability to rapidly identify a pathogen could facilitate more effective treatment strategies, reducing the unnecessary use of antibiotics. Current diagnostics of bacterial infections often require cell growth, and are therefore less capable of informing timely treatment options. Here, we describe the development of a rapid assay (<45 min) to identify Gram-positive bacteria directly in human serum without any growth or isolation required. We have developed a fluorescence-based immunoassay specifically targeting the Gram-positive bacterial biomarker lipoteichoic acid (LTA). LTA is an amphiphilic molecule with both hydrophobic and hydrophilic portions. Our approach, termed membrane insertion, exploits the amphiphilic biochemistry of the molecule by allowing it to passively insert itself into a lipid bilayer, thereby trapping it for subsequent probing with a fluorescently labeled α-LTA antibody. Using this assay, we have successfully detected less than 10 µg/ml of LTA directly in spiked human serum. We intend to combine this rapid assay with others currently in development into a diagnostic tool for point-of-care detection of bacterial pathogens.

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Pathogen detection using single mode planar optical waveguides

Cited by 41 publications

References 25 publications

Detection of Intact Influenza Viruses using Biotinylated Biantennary S-Sialosides

Detection of Intact Influenza Viruses using Biotinylated Biantennary S-Sialosides

Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis

Membrane Insertion for Direct Detection of Lipoteichoic Acid

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