Pathogen Contamination in Dairy Manufacturing Environments

Parkar, Shanthi G.; Flint, Steve; Teh, Koon Hoong; Brooks, J.; Bremer, Phil

doi:10.1002/9781118876282.ch9

Cited by 3 publications

(6 citation statements)

References 177 publications

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“…The permanence of these pathogens in the production line is of concern to the dairy industry, since at some point these species can be transferred from the processing surfaces to the food, remaining in the final product (Parkar et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…), some studies report the difficulty of L. monocytogenes in forming biofilms in dual‐species biofilms with E. faecium (Parkar et al . ; Teh et al . ).…”

Section: Resultsmentioning

confidence: 99%

“…The ability of L. monocytogenes to form biofilm depends on factors such as stain and serotype, origin, temperature, nutrient level, previous exposure to sublethal stressors (acid), topography and composition of the surface. Although the presence of other bacteria may increase the ability of L. monocytogenes to form biofilms (Bremer et al 2001), some studies report the difficulty of L. monocytogenes in forming biofilms in dual-species biofilms with E. faecium (Parkar et al 2015;Teh et al 2015).…”

Section: Resultsmentioning

confidence: 99%

“…This is possibly because of the beneficial exchange of nutrients and of the removal of the antagonistic substances produced by neighbouring cells by diffusion (Costerton et al 1994). The permanence of these pathogens in the production line is of concern to the dairy industry, since at some point these species can be transferred from the processing surfaces to the food, remaining in the final product (Parkar et al 2015).…”

Section: Resultsmentioning

confidence: 99%

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Formation and dispersal of biofilms in dairy substrates

Alonso

Kabuki

2019

Int J of Dairy Tech

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The formation and dispersion of biofilms in the dairy industry is a problem because it increases cross‐contamination, affecting the shelf life of the products and their safety. The objective of this study was to evaluate the influence of different dairy substrates (cows’ milk and whey protein) on the formation and dispersion of Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus in two biofilm systems (mono‐species and multi‐species) on stainless steel at 25 °C. The dominant behaviour of E. faecalis occurred in most of the tests on mono‐species and multi‐species biofilms. A greater dispersion of biofilm cells was observed in skimmed milk.

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Section: Resultsmentioning

confidence: 97%

“…), some studies report the difficulty of L. monocytogenes in forming biofilms in dual‐species biofilms with E. faecium (Parkar et al . ; Teh et al . ).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Formation and dispersal of biofilms in dairy substrates

Alonso

Kabuki

2019

Int J of Dairy Tech

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“…and E. coli often comes in contact either directly with the animals or milking equipment, resulting in the development of biofilm [ 34 , 35 ]. However, this causes contamination of the dairy products and diseases among affected animals, as well as serving as potential zoonosis [ 36 , 37 ].…”

Section: Microbial Food Contact Surface In Veterinary Medicinementioning

confidence: 99%

Intrigues of biofilm: A perspective in veterinary medicine

Abdullahi¹,

Igwenagu²,

Muazu³

et al. 2016

Vet World

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Biofilm has a tremendous impact in the field of veterinary medicine, especially the livestock industry, leading to a serious economic loss. Over the years, little attention has been given to biofilm in animals with most of the research geared toward human biofilm diseases. The greatest challenge posed by biofilm is in its incredible ability to resist most of the currently existing antibiotics. This mystery can best be demystified through understanding the mechanism of the quorum sensing which regulate the pathophysiology of biofilm. Ability of biofilm formation in a variety of inanimate surfaces such as animal food contact surfaces is responsible for a host of biofilm diseases affecting animals and humans. In this review, we highlighted some of the challenges of biofilm in livestock and food industries. Also highlighted are; mechanisms of biofilm development, best diagnostic approach and possible novel therapeutic measures needed to combat the menace of biofilm in veterinary medicine.

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Comparative Study of Different Sampling Methods of Biofilm Formed on Stainless-Steel Surfaces in a CDC Biofilm Reactor

et al. 2022

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The formation of biofilms in dairy processing plants can reduce equipment efficiency, contribute to surface deterioration, and contaminate dairy products by releasing the microorganisms they contain, which may cause spoilage or disease. However, a more representative identification of microbial communities and physico-chemical characterization requires to detach and recover adequately the entire biofilm from the surface. The aim of this study is to develop an efficient technique for in-plant biofilm sampling by growing a strain of Pseudomonas azotoformans PFl1A on stainless-steel surface in a dynamic CDC biofilm reactor system using tryptic soy broth (TSB) and milk as growth media. Different techniques, namely, swabbing, scraping, sonic brushing, synthetic sponge, and sonicating synthetic sponge were used and the results were compared to a standard ASTM International method using ultrasonication. Their efficiencies were evaluated by cells enumeration and scanning electron microscopy. The maximum total viable counts of 8.65 ± 0.06, 8.75 ± 0.08, and 8.71 ± 0.09 log CFU/cm2 were obtained in TSB medium using scraping, synthetic sponge, and sonicating synthetic sponge, respectively, which showed no statistically significant differences with the standard method, ultrasonication (8.74 ± 0.02 log CFU/cm2). However, a significantly (p < 0.05) lower cell recovery of 8.57 ± 0.10 and 8.60 ± 0.00 log CFU/cm2 compared to ultrasonication were achieved for swabbing and sonic brushing, respectively. Furthermore, scanning electron microscopy showed an effective removal of biofilms by sonic brushing, synthetic sponge, and sonicating synthetic sponge; However, only the latter two methods guaranteed a superior release of bacterial biofilm into suspension. Nevertheless, a combination of sonication and synthetic sponge ensured dislodging of sessile cells from surface crevices. The results suggest that a sonicating synthetic sponge could be a promising method for biofilm recovery in processing plants, which can be practically used in the dairy industries as an alternative to ultrasonication.

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Pathogen Contamination in Dairy Manufacturing Environments

Cited by 3 publications

References 177 publications

Formation and dispersal of biofilms in dairy substrates

Formation and dispersal of biofilms in dairy substrates

Intrigues of biofilm: A perspective in veterinary medicine

Comparative Study of Different Sampling Methods of Biofilm Formed on Stainless-Steel Surfaces in a CDC Biofilm Reactor

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