Pathobiology of Enterocytozoon hepatopenaei (EHP) in shrimp: Diagnosis and interpretation from the gut bacterial community

Shen, Huiyu; Zhang, Xuechen; Dong, Qian; Chen, Jiong; Xiong, Jinbo

doi:10.1016/j.aquaculture.2022.738169

Cited by 10 publications

(2 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Owing to the economic value and high nutrition, shrimp ( Litopenaeus vannamei , Penaeidae) is the most widely cultivated shrimp species globally (Dong et al, 2021). As the results of high density and stressed conditions, shrimp aquaculture is threatened by diverse diseases such as acute hepatopancreatic necrosis disease (AHPND; Kumar et al, 2020), white faeces syndrome (WFS; Lu et al, 2020), and Enterocytozoon hepatopenaei (Shen et al, 2022). Shrimp lack an adaptive immune system for natural protection, thus hampering the conventional vaccination against pathogens (Johnson et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Consistent features of the gut microbiota in response to diverse shrimp Litopenaeus vannamei diseases: A meta‐analysis

Mao,

Lu,

Chen

et al. 2023

Fish and Fisheries

Self Cite

View full text Add to dashboard Cite

Dysbiosis in the gut microbiota has been intimately implicated in shrimp (Litopenaeus vannamei, Penaeidae) diseases. However, considering the variety of shrimp diseases and the variability in experimental conditions, studies addressing common features of the gut microbiota−shrimp disease relationship are limited. Through an unbiased subject‐level meta‐analysis framework, 463 shrimp gut bacterial communities from 27 studies were re‐analysed, including six lifestages and eight diseases of shrimp, with the causal agents of viral, bacterial, eukaryotic, and unknown pathogens. Shrimp lifestages and diseases were the predominant factors governing the gut microbiota. After ruling out the top lifestage‐ and disease‐specific discriminatory amplicon sequence variants (ASVs) from the gut microbiota, the top 27 disease common‐discriminatory ASVs were identified, contributing to an overall accuracy of 95.9% in diagnosing shrimp health status. By using these optimisation procedures, the accuracy of our diagnosis model was unbiased by shrimp lifestage, specific disease, sampling size, hypervariable region and sequencing platform. The shrimp eight diseases consistently and significantly increased the relative importance of stochasticity, the relative abundance of pathogenic potentials and diversified core ASVs, whereas decreased the diversity and stability of gut microbiota. Collectively, these findings illustrate the effectiveness of meta‐analysis in determining the robust and common features of the shrimp gut microbiota in response to diverse diseases. In particular, disease common‐discriminatory ASVs could accurately diagnose shrimp health status, although the data are divergent in biotic and technical variances.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Recent studies have identified shrimp gut bacterial indicators for diagnosing the incidence of single disease by excluding the lifestage effect (Shen et al, 2022; Xiong, Zhu, et al, 2017). However, the shrimp gut microbiotas seem to divergently respond to different diseases through a disease‐specific response.…”

Section: Introductionmentioning

confidence: 99%