Paternity tests in Mexico: Results obtained in 3005 cases

García-Aceves, Mayra Elizabeth; Rentería, O. Romero; Díaz-Navarro, Xochitl X.; Rangel-Villalobos, Héctor

doi:10.1016/j.jflm.2018.02.003

Cited by 14 publications

(6 citation statements)

References 34 publications

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“…The ratio of repeat gains and losses was relatively balanced (5:9), while four mutations could not be assigned. The average paternal mutation rate estimated across all loci was 0.0016 (95% CI 0.0009-0.0025) per locus per gamete per generation, which is mostly in agreement with previous studies [33,34]. The genotype details of paternity inconsistencies that resulted from mutations in 16 autosomal microsatellite loci studied are described in Table 1 (unpublished data).…”

Section: Astr Typing Resultssupporting

confidence: 88%

“…The 16 forensic loci were sufficient to provide positive proof (strong evidence) of paternity, offering high discriminating power in only 15 out of 16 triplet cases, and more genetic information and accurate statistical analyses for achieving confident results are required. According to García-Aceves et al, the implementation of ≥ 20 aSTRs as the routine battery of markers for paternity testing labs allows for obtaining sound conclusions to solve the large majority of motherless cases [33]. In addition, as most DNA-typing applications have legal and ethical implications and there is a particular need for high reliability and high discrimination power in cases where fatherhood cannot be confirmed or ruled out with statistical certainty, the focus should be shifted to the application of alternative and often more discriminative and polymorphic markers [40][41][42].…”

Section: Discussionmentioning

confidence: 99%

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Utilizing Massively Parallel Sequencing (MPS) of Human Leukocyte Antigen (HLA) Gene Polymorphism to Assess Relatedness in Deficiency Parentage Testing

Kouniaki,

Fotopoulos,

Tarassi

et al. 2024

Genes

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In the realm of DNA testing with legal implications, the reliability and precision of genetic markers play a pivotal role in confirming or negating paternity claims. This study aimed to assess the potential utility of human leukocyte antigen (HLA) gene polymorphism through massively parallel sequencing (MPS) technology as robust forensic markers for parentage testing involving genetic deficiencies. It sought to redefine the significance of HLA genes in this context. Data on autosomal short tandem repeat (aSTR) mutational events across 18 paternity cases involving 16 commonly employed microsatellite loci were presented. In instances where traditional aSTR analysis failed to establish statistical certainty, kinship determination was pursued via HLA genotyping, encompassing the amplification of 17 linked HLA loci. Within the framework of this investigation, phase-resolved genotypes for HLA genes were meticulously generated, resulting in the definition of 34 inherited HLA haplotypes. An impressive total of 274 unique HLA alleles, which were classified at either the field 3 or 4 level, were identified, including the discovery of four novel HLA alleles. Likelihood ratio (LR) values, which indicated the likelihood of the observed data under a true biological relationship versus no relationship, were subsequently calculated. The analysis of the LR values demonstrated that the HLA genes significantly enhanced kinship determination compared with the aSTR analysis. Combining LR values from aSTR markers and HLA loci yielded conclusive outcomes in duo paternity cases, showcasing the potential of HLA genes and MPS technology for deeper insights and diversity in genetic testing. Comprehensive reference databases and high-resolution HLA typing across diverse populations are essential. Reintegrating HLA alleles into forensic identification complements existing markers, creating a potent method for future forensic analysis.

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Section: Astr Typing Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Utilizing Massively Parallel Sequencing (MPS) of Human Leukocyte Antigen (HLA) Gene Polymorphism to Assess Relatedness in Deficiency Parentage Testing

Kouniaki,

Fotopoulos,

Tarassi

et al. 2024

Genes

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show abstract

“…We observed an overall parentage exclusion rate of 31.16%. The paternity exclusion rate was 32.33 which is comparable to our previous findings 10 but slightly higher than other reported rates 9 , 11 – 13 . This could be because exclusion rates were estimated from cases that arose from doubts regarding biological parenthood.…”

Section: Discussionsupporting

confidence: 86%

Analysis of data and common mutations encountered during routine parentage testing in Zimbabwe

Thelingwani,

Jonhera,

Masimirembwa

2024

Sci Rep

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We analyzed parentage data collected over a ten-year period in a Zimbabwean DNA testing laboratory. Parentage case types, prevalence, exclusion data, mutations rates and observed genotyping irregularities were analyzed. We report analysis results from 1303 cases. DNA extraction and STR typing was conducted using standard commercial kits. Paternity was the most requested test (87.37%) followed by the indirect biological kinship tests (7.01%). Duo paternity (motherless) was the most common paternity test for both regular and court cases. We observed 367 paternity exclusions from 1135 cases, giving an overall paternity exclusion rate of 32.33%. Maternity had the lowest exclusion rate (8.33%), with criminal cases having the highest paternity (61.11%) and maternity (33.33%) exclusion rates. The number of mismatched STR loci ranged from 2–12 for duo cases and 4–18 for the trio cases. FGA, D2S1338, D18S51 and D2S441 were the most informative markers for exclusion. We detected 30 mutations out of 837 cases with an estimated paternal and maternal mutation rate of 0.0021 and 0.0011 respectively. Triallelic patterns were only observed at the TPOX locus with allele 10 and 11 being the extra alleles transmitted. Our report provides forensic parameters which can improve parentage and forensic analysis in Zimbabwe.

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“…Another previous study performed in populations of Southern, Southeastern and Central Brazilian reported D10S1237 and D19S253 as those with the highest mutation rates, and no mutations in TH01 and TPOX. The most polymorphic loci in our study vWA, D18S51, FGA, and D21S11 presented higher mutation rates in agreement with previous studies .…”

Section: Slippage Mutations Observed In 5171 Paternity Cases From Indsupporting

confidence: 93%

“…We confirmed 189 (97.9%) mutations as one‐step and four (2.1%) as two‐step, the latter at locus FGA (22 > 24 or 26 > 24; 27 > 25), CSF1PO (12 > 14), and D13S317 (11 > 9). Higher numbers of slippage mutations were observed in the most polymorphic loci, matching the data presented by other studies .…”

Section: Slippage Mutations Observed In 5171 Paternity Cases From Indsupporting

confidence: 90%

Slippage mutation rates in 15 autosomal short tandem repeat loci for forensic purposes in a Southeastern Brazilian population

Hamester

Silva²,

Leboute

et al. 2019

Electrophoresis

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Slippage mutation rates in 15 autosomal short tandem repeat loci for forensic purposes in a Southeastern Brazilian populationWell-defined estimates of mutation rates in highly polymorphic tetranucleotide STR loci are a prerequisite for human identification in genetics laboratory routines useful for civil and criminal investigations. Studying 15 autosomal STR loci of forensic interest (CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, TH01, TPOX, and vWA), we detected 193 slippage mutations (189 one-step and four two-step mutations) in 148 875 parent-child allelic transfers from 5171 paternity cases with true biological relationship (15 096 individuals; 4754 trios and 417 duos; 9925 meiosis) from the state of São Paulo, a very representative population of Brazil. The overall mutation rate was 1.3 × 10 −3 and the highest rates were observed at loci vWA (2.8 × 10 −3 ), FGA and D18S51 (2.7 × 10 −3 for both), while loci TH01 and TPOX did not present any mutations. The mean slippage mutation rate of paternal origin (1.8 × 10 −3 ) was six times higher than that observed for maternal origin (0.3 × 10 −3 ).

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Paternity tests in Mexico: Results obtained in 3005 cases

Cited by 14 publications

References 34 publications

Utilizing Massively Parallel Sequencing (MPS) of Human Leukocyte Antigen (HLA) Gene Polymorphism to Assess Relatedness in Deficiency Parentage Testing

Utilizing Massively Parallel Sequencing (MPS) of Human Leukocyte Antigen (HLA) Gene Polymorphism to Assess Relatedness in Deficiency Parentage Testing

Analysis of data and common mutations encountered during routine parentage testing in Zimbabwe

Slippage mutation rates in 15 autosomal short tandem repeat loci for forensic purposes in a Southeastern Brazilian population

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