Paternally-acting canonical RNA-directed DNA methylation pathway genes sensitize Arabidopsis endosperm to paternal genome dosage

Satyaki, Rajavasireddy P; Gehring, Mary

doi:10.1101/527853

Cited by 13 publications

(36 citation statements)

References 79 publications

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“…However, while Martinez et al (2018) did not find a suppressive effect when using mutants in RdDM components such as RDR2 and NRPE1, Satyaki and Gehring (2019) found those mutants to suppress hybrid seed failure. The difference between both studies lies in the use of tetraploid RdDM mutants by Satyaki and Gehring (2019), while RdDM mutants introgressed into omission of second division 1 (osd1) were used by Martinez et al (2018). Loss of OSD1 suppresses the second meiotic division, leading to unreduced gamete formation (d’Erfurth et al, 2009).…”

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 93%

“…Recent work from our and other groups revealed that disruption of NRPD1 suppresses the hybridization barrier between plants of different ploidy grades (Martinez et al, 2018; Satyaki and Gehring, 2019). However, while Martinez et al (2018) did not find a suppressive effect when using mutants in RdDM components such as RDR2 and NRPE1, Satyaki and Gehring (2019) found those mutants to suppress hybrid seed failure. The difference between both studies lies in the use of tetraploid RdDM mutants by Satyaki and Gehring (2019), while RdDM mutants introgressed into omission of second division 1 (osd1) were used by Martinez et al (2018).…”

Section: Discussionmentioning

confidence: 93%

“…The 24- nt siRNAs preferentially associate with ARGONAUTE4 (AGO4) and guide DRM2 to its targets by associating with Pol V transcripts (Cao and Jacobsen, 2002; Zilberman et al, 2003; Wierzbicki et al, 2009). Recent studies further uncovered that Pol IV is required for the accumulation of 21/22-nt epigenetically activated siRNAs (easiRNAs) in pollen and establishes a hybridization barrier between plants of different ploidy grades (Martinez et al, 2018; Borges et al, 2018; Satyaki and Gehring, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Functional role of Polymerase IV during pollen development inCapsella

Wang

Butel

Santos‐González

et al. 2019

Preprint

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In Arabidopsis thaliana, the DNA-dependent RNA polymerase IV (Pol IV) is required for the formation of transposable element (TE)-derived small RNA (sRNA) transcripts. These transcripts are processed by DICER-LIKE 3 into 24-nt small interfering RNAs (siRNAs) that guide RNA-dependent DNA methylation. In the pollen grain, Pol IV is also required for the accumulation of 21/22-nt epigenetically-activated siRNAs (easiRNAs) that likely silence TEs by post-transcriptional mechanisms. Despite this proposed functional role, loss of Pol IV function in Arabidopsis does not cause a discernable pollen defect. Here, we show that loss of NRPD1, encoding the largest subunit of Pol IV in the Brassicaceae Capsella rubella, causes post-meiotic arrest of pollen development at the microspore stage. As in Arabidopsis, all TE-derived siRNAs were depleted in Capsella nrpd1 microspores. In wild-type background, we found that the same TEs produced 21/22-nt and 24-nt siRNAs, leading us to propose that Pol IV is generating the direct precursors for 21-24-nt siRNAs, which are targeted by different DICERs. Arrest of Capsella nrpd1 microspores was accompanied by deregulation of genes targeted by Pol IV-dependent siRNAs. The distance of TEs to genes was much closer in Capsella rubella compared to Arabidopsis thaliana, providing a possible explanation for the essential role of Pol IV for pollen development in Capsella. Our study in Capsella uncovers a functional requirement of Pol IV in microspores, emphasizing the relevance of investigating different plant models.One-sentence summaryLoss of Polymerase IV function in Capsella rubella causes microspore arrest, revealing an important functional role of Polymerase IV during pollen development.The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Claudia Kohler (Claudia.Kohler@slu.se)

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Functional role of Polymerase IV during pollen development inCapsella

Wang

Butel

Santos‐González

et al. 2019

Preprint

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“…In Arabidopsis , the triploid block is established upon crosses of a pollen donor forming 2n pollen with a diploid maternal plant. Depletion of the major Pol IV subunit NRPD1A or the miRNA gene MIR845B suppresses the triploid block response 9,37-39 . To test whether the miRNA populations loaded in AGO1 or AGO5 are responsible for establishing the triploid block, we created tetraploid versions of the AGO1 weak allele ago1-27 and of the AGO5 alleles ago5-1 and ago5-5 and performed crosses with a wt diploid mother.…”

Section: Resultsmentioning

confidence: 99%

MicroRNA function transitions from regulating developmental genes to transposable elements during the maturation of pollen

Oliver

Annacondia

Wang

et al. 2019

Preprint

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AbstractmicroRNAs play important roles to control the development of eukaryotic organisms. Both animal and plant microRNAs are essential for the spatio-temporal regulation of development but together with this role, plant microRNAs also control transposable elements and stimulate the production of epigenetically-active small interfering RNAs. This last role is evident in the plant male gamete containing structure, the male gametophyte or pollen grain, but how the dual role of plant microRNAs is integrated during its development is unknown. Here, we provide a detailed analysis of microRNA dynamics during pollen development and their genic and transposable element targets using small RNA and mRNA cleavage (PARE) high-throughput sequencing. Furthermore we uncover the microRNAs loaded in the two main Argonaute proteins in the mature pollen grain, AGO1 and AGO5. Our results indicate that the developmental progression from microspore to mature pollen grain is characterized by a reprogramming from microRNAs focused on the control of development to microRNAs regulating transposable element control.

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First the seed: Genomic advances in seed science for improved crop productivity and food security

Dwivedi¹,

Spillane

Lopez

et al. 2021

Crop Science

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Seeds are valuable sources of carbohydrates, lipids, proteins, fibers, minerals, and vitamins. They provide energy and nutrition to germinating seedlings, food to humans, feed to livestock and feedstocks to industry. High-throughput analyses of gene expression in crops has identified many candidate genes associated with seed dormancy, longevity, germination, and vigor. In this review, we cover the latest research focusing on such key seed traits. Transcriptome analyses of time-courses of seed filling have identified sets of genes expressed at different stages of this process. The potential role of epigenetics (including seed-endosperm imprinted genes) in regulating seed development and chemistry is highlighted herein. We also discuss how advances in genomics and seed biology are facilitating the unravelling of associations between seed traits with genebank accessions and gene sequences, including how functional research can accelerate the discovery of allelic variants. Such knowledge of functional effects relating to gene variants is necessary for more efficient and cost-effective management of genetic resources or for redesigning crops with specific seed characteristics. For instance, genebank curators may assess seed viability by monitoring changes in gene expression of biomarker genes in dry seed samples to decide germplasm regeneration and assess genetic integrity of collections by monitoring changes in This article is protected by copyright. All rights reserved. diversity and allele frequencies between samples of same accession stored in genebanks. We highlight that resistance to preharvest sprouting can be enhanced through genomicsassisted breeding in otherwise nondormant rice and wheat cultivars, while pimt, another valuable marker for seed longevity, may be deployed to enhance seed vigor in crops.

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Paternally-acting canonical RNA-directed DNA methylation pathway genes sensitize Arabidopsis endosperm to paternal genome dosage

Cited by 13 publications

References 79 publications

Functional role of Polymerase IV during pollen development inCapsella

Functional role of Polymerase IV during pollen development inCapsella

MicroRNA function transitions from regulating developmental genes to transposable elements during the maturation of pollen

First the seed: Genomic advances in seed science for improved crop productivity and food security

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