Patchouli alcohol ameliorates dextran sodium sulfate-induced experimental colitis and suppresses tryptophan catabolism

Qu, Chang; Yuan, Zhongwen; Yu, Xiu-Ting; Huang, Yanfeng; Yang, Guanghua; Chen, Jiannan; Lai, Xiaoping; Su, Zi‐Ren; Zeng, Hong‐Yan; Xie, Ying; Zhang, Xiaojun

doi:10.1016/j.phrs.2017.04.017

Cited by 74 publications

(35 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been proposed that the tryptophan reduction during the inflammation is caused by the elevated activity of kynurenine pathway that leads to the tryptophan degradation in favor of nicotinamide adenine dinucleotide (NAD+). The key regulators of this process are the pro-inflammatory cytokines and indoleamine 2,3-dioxygenase 1 (IDO1), one of the enzymes that catalyze the rate-limiting step in the kynurenine pathway [47,48,66,67]. In agreement with these studies, we observed a strong up-regulation of the Ido1 gene expression during chronic colitis ( Figure 3E).…”

Section: Discussionsupporting

confidence: 86%

“…For instance, it has been noticed that the patients with acute and chronic forms of IBD have reduced tryptophan level in the blood plasma [65,66]. Moreover, the targeted analysis revealed that the plasma level of tryptophan significantly decreased in DSS-induced mouse model of acute colitis [47,48]. Our data support these findings, since we observe the impressive 30% reduction of serum tryptophan upon chronic DSS treatment.…”

Section: Discussionsupporting

confidence: 85%

“…Chronic colitis resulted in about 30% loss of serum tryptophan, which was restored to the control level with fucose addition ( Figure 3D). Since it has been previously shown that the serum tryptophan depletion during colitis is associated with the up-regulation of indoleamine 2,3-dioxygenase 1 (Ido1) gene expression, one of the enzymes that catalyze the rate-limiting step in the kynurenine pathway [47,48], we measured the expression level of this enzyme in the intestinal samples taken from the test animals. Two-way ANOVA revealed a significant effect of DSS on Ido1 expression (p < 0.001) and, in agreement with the previous studies, chronic DSS treatment resulted in a significant up-regulation of the Ido1 gene expression (p < 0.001, Student's t-test, Figure 3E).…”

Section: Fucose Normalizes the Decrease Of Blood Tryptophan Level Upomentioning

confidence: 99%

See 2 more Smart Citations

Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

et al. 2020

View full text Add to dashboard Cite

Growing evidence suggests that intestinal mucosa homeostasis impacts immunity, metabolism, the Central Nervous System (CNS), and behavior. Here, we investigated the effect of the monosaccharide fucose on inflammation, metabolism, intestinal microbiota, and social behavior in the Dextran Sulfate Sodium (DSS)-induced chronic colitis mouse model. Our data show that chronic colitis is accompanied by the decrease of the serum tryptophan level and the depletion of the intestinal microbiota, specifically tryptophan-producing E. coli and Bifidobacterium. These changes are associated with defects in the male mouse social behavior such as a lack of preference towards female bedding in an odor preference test. The addition of fucose to the test animals' diet altered the bacterial community, increased the abundance of tryptophan-producing E. coli, normalized blood tryptophan levels, and ameliorated social behavior deficits. At the same time, we observed no ameliorating effect of fucose on colon morphology and colitis. Our results suggest a possible mechanism by which intestinal inflammation affects social behavior in male mice. We propose fucose as a promising prebiotic, since it creates a favorable environment for the beneficial bacteria that promote normalization of serum tryptophan level and amelioration of the behavioral abnormalities in the odor preference test.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 85%

Section: Fucose Normalizes the Decrease Of Blood Tryptophan Level Upomentioning

confidence: 99%

See 1 more Smart Citation

Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Elucidating the molecular mechanism how to e ciently decrease colonic epithelial cell apoptosis and how to repair mucosal tissues has become the focus of therapy of UC [44]. The increased number of apoptotic epithelial cells induced by enhanced Bax but attenuated Bcl-2 during active UC may lead to a defective epithelial barrier and result in pathogenic microorganism in ltration [35,45]. Our results indicated that colitis rats had more apoptotic cells, evidenced by the increased Bax and cleaved caspase3 but reduced anti-apoptosis protein Bcl-2.…”

Section: Discussionmentioning

confidence: 99%

Anemoside B4 ameliorates TNBS-induced colitis involved suppressing the S100A9/NF-κB signaling pathway

Zhang¹,

Zha²,

Shen³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Despite the increased morbidity of ulcerative colitis (UC) in the developing countries, available treatments remain unsatisfactory. Therefore, it is urgent to discover more effective therapeutic strategies. Pulsatilla chinensis was widely used for the treatment of inflamed intestinal diseases including UC for thousands of years in China. However, it is unclear which compound in P. chinensis is responsible for the therapeutic effect. Our previous study reported that anemoside B4, the most abundant triterpenoid saponin isolated from P. chinensis, exerts anti-inflammatory and antioxidant effects. Methods Here, we used the 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis rat model to evaluate the therapeutic effect of anemoside B4. Blood samples of colitis rat were collected for hematology analysis. The effects of anemoside B4 on inflammation-associated mediators were investigated by Enzyme-linked immunosorbent assay (ELISA) and hematoxylin and eosin staining (HE) staining. Cell proliferation or apoptosis was measured by immunofluorescence technique. The mechanisms of anemoside B4 was investigated using label-free quantitative proteomics. The level of proteins was quantified by western blotting. mRNA expression was quantified by quantitative real-time RT-PCR. Results The results showed that anemoside B4 ameliorated TNBS-induced colitis symptoms, including tissue damage, inflammatory cell infiltration, and pro-inflammatory cytokine production, apoptosis and slowed proliferation in the colon. Quantitative proteomic analyses discovered that 56 proteins were significantly altered by anemoside B4 in the TNBS-induced rats. These proteins were mainly clustered in tricarboxylic acid cycle (TCA) cycle and respiratory electron transport chain. Among the altered proteins, S100A9 is one of the most significantly downregulated proteins and associated with NF-κB and MAPK signaling pathways in the pathogenesis of UC. Further experiments revealed that anemoside B4 suppresses the expression of S100A9 and its downstream genes including TLR4, NF-κB, and p-JNK in colon. In vitro, S100A9 protein could active NF-κB signaling pathway in human intestinal epithelial Caco-2 cells. However, anemoside B4 could inhibit the NF-κB signaling pathway induced by S100A9 protein. Besides, it also inhibited active of NF-κB signaling pathway stimulated by LPS or IL-6. Conclusions Our results demonstrate that anemoside B4 prevents TNBS-induced colitis involved inhibiting the NF-κB signaling pathway through inactivating S100A9 suggesting that anemoside B4 is a promising therapeutic candidate for colitis.

show abstract

“…For the first time, our group reported that patchouli alcohol showed anti-inflammatory and anticancer activities in colon cancer cells. 16 Patchouli alcohol ameliorated DSS-induced mice acute colitis by suppressing inflammation 17 and attenuated atherosclerosis, possibly by inhibiting macrophage infiltration and its inflammatory responses. 18 However, the effect of patchouli alcohol on obesity has not been explored so far.…”

Section: Introductionmentioning

confidence: 97%

Patchouli Alcohol, a Compound from Pogostemon cablin, Inhibits Obesity

Lee

Kong

Lee

2020

Journal of Medicinal Food

View full text Add to dashboard Cite

Obesity predisposes people to a variety of chronic metabolic diseases. Identification of natural factors that prevent the development of obesity is likely to be the most successful means of ameliorating the current obesity epidemic. Patchouli alcohol is a sesquiterpene alcohol found in Pogostemon cablin and possesses health benefit activities. This study was designed to examine if patchouli alcohol affects adipogenesis, and investigates the underlying mechanisms whereby patchouli alcohol exerts antiobesity effect. 3T3-L1 adipocytes were differentiated with treatment of different concentrations of patchouli alcohol. An in vivo study was performed to test the effect of patchouli alcohol gavage on a high-fat diet (HFD)-induced obesity. Treatment of patchouli alcohol reduced lipid accumulation in 3T3-L1 adipocytes in a dose-dependent manner without toxicity. Regarding mechanism, treatment of patchouli alcohol reduced expression of peroxisome proliferator-activated receptor-gamma (PPARc) and CCAAT-enhancer-binding protein-alpha (C/EBPa) and increased expression of total and active b-catenin in 3T3-L1 adipocytes. Oral gavage of patchouli alcohol led to a significant reduction of body weight and fat accumulation in the mice fed with HFD. Transcriptome analysis indicates that smad7 is most highly activated gene in patchouli alcohol-treated 3T3-L1 cells. Patchouli alcohol possesses health benefit effect through inhibiting adipogenesis and fat tissue development.

show abstract

Patchouli alcohol ameliorates dextran sodium sulfate-induced experimental colitis and suppresses tryptophan catabolism

Cited by 74 publications

References 48 publications

Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

Anemoside B4 ameliorates TNBS-induced colitis involved suppressing the S100A9/NF-κB signaling pathway

Patchouli Alcohol, a Compound from Pogostemon cablin, Inhibits Obesity

Contact Info

Product

Resources

About