Patch Closure of Muscular Ventricular Septal Defects With a New Hybrid Therapy in a Pig Model

Kozlik‐Feldmann, Rainer; Lang, Nora; Aumann, Regine; Lehner, Anja; Rassoulian, D.; Sodian, Ralf; Schmitz, Christoph; Hinterseer, Martin; Hinkel, Rabea; Thein, E.; Freudenthal, Franz; Vasylev, Nikolay V.; Nido, Pedro J. del; Netz, Heinrich

doi:10.1016/j.jacc.2007.10.068

Cited by 14 publications

(13 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The structure and physiology of swine are similar to humans, and pigs are a readily available source of mesenchymal stem cells for investigation (Alam et al 2008;Kozlik-Feldmann et al 2008). Subcutaneous back fat and bone marrow from femurs were harvested from three castrated Yorkshire crossbred male pigs, with ADSC and BMSC being isolated and cultured in vitro, as previously reported (Monaco et al 2009).…”

Section: Adsc and Bmsc Isolation And Culturementioning

confidence: 99%

Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels

et al. 2010

View full text Add to dashboard Cite

Advances in bioengineering, material chemistry, and developmental biology have led to the design of three-dimensional (3D) culture systems that better resemble the surrounding structure and chemistry of the in situ niches of cells in tissues. This study was designed to characterize and compare porcine adipose-derived stem cells (ADSC) and bone-marrow-derived stem cells (BMSC) induced to differentiate toward osteogenic and adipogenic lineages in vitro by using a 3D alginate hydrogel. The morphology and gene expression of the two cell populations during differentiation were analyzed. Both ADSC and BMSC showed morphological evidence of osteogenic and adipogenic differentiation. Expression patterns of genes characteristic of the onset of osteogenic differentiation (ALP, COL1A1, SPARC, SPP1) were low at the beginning of culture and generally increased during the period of differentiation up to 28 days in culture. Expression of genes associated with adipogenic differentiation (ACSL1, ADFP, ADIPOQ, CD36, DBI, DGAT2, PPARG, SCD) was consistently increased in ADSC cultured in alginate hydrogel relative to the start of differentiation. However, adipogenic gene expression of BMSC cultured in alginate hydrogel was more limited when compared with that of ADSC. Evaluation of cell numbers (via the MTT staining assay) suggested a greater viability of BMSC under osteogenic conditions in alginate hydrogels than under adipogenic conditions, whereas ADSC had greater viability under adipogenic conditions than under osteogenic conditions. This study thus provides an important initial evaluation of ADSC and BMSC seeded and differentiated toward the osteogenic and adipogenic cell lineages in a 3D alginate hydrogel in vitro.

show abstract

Section: Adsc and Bmsc Isolation And Culturementioning

confidence: 99%

Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The use of an animal model, such as swine, that adequately simulates the structure and physiology of humans, provides a readily available source of mesenchymal stem cells for research, and allows for improvements in therapeutic protocols [18][19][20]. Pigs have been used as a large animal model for studying human clinical therapies as diverse as heart surgery [18], genetic disorders [21], and drug testing [22].…”

Section: Introductionmentioning

confidence: 99%

Morphological and Transcriptomic Comparison of Adipose and Bone Marrow Derived Porcine Stem Cells

Monaco¹,

Lima²,

Bionaz³

et al. 2009

TOTERMJ

View full text Add to dashboard Cite

Abstract:In the present study we provided a morphological and transcriptomic comparison of adult porcine adiposederived stem cells (ADSC) and bone marrow-derived stem cells (BMSC) as they differentiated in vitro towards the osteogenic and adipogenic lineages for up to 4 weeks. The long term goal of this comparison is to assess the possibility of using ADSC as a potential alternative to BMSC as a source of autologous adult stem cells in human therapies. Our data indicated that ADSC can differentiate into osteocytes and adipocytes similar to BMSC but with some differences. During the osteogenic differentiation both cell types went through morphological changes; however, while ADSC formed predominately osteogenic islands (nodules) in the culture dish, BMSC formed a continuous osteogenic sheet of small nodules. Transcriptomic analysis revealed that both cell types responded to the osteogenic induction. However, BGLAP mRNA expression did not increase in ADSC suggesting, together with the percentage area stained observed for Alizarin Red and von Kossa in ADSC, a lesser mineralization of bone matrix in this cell type compared to BMSC. During the adipogenic induction ADSC as well as BMSC were able to achieve the morphological and transcriptome changes characteristic of the adipogenic lineage. After 7 days of differentiation the expression patterns of DGAT2 and ADFP became greater in ADSC versus BMSC, which agreed with the larger lipid droplets formation observed in the ADSC by Oil Red O staining. Our findings represent an important step towards the assessment of using ADSC as an alternative to BMSC in therapeutic applications.

show abstract

“…In a paper from Germany, Kozlik-Feldmann et al (148) presented a unique hybrid model for ventricular septal defect closure in pigs that underwent a left anterolateral thoracotomy and had muscular ventricular septal defects created by a punch. A closure therapy consisting of a patch with a nitinol frame was advanced retrograde through the carotid artery into the LV, and an instrument very much like a stapler was passed into the ventricle to fix the patch on the septum under echocardiographic and fluoroscopic guidance, after which the nitinol frame was removed.…”

Section: Congenital Heart Diseasementioning

confidence: 99%

Highlights of the Year in JACC 2008

DeMaria

Ben‐Yehuda

Bax

et al. 2009

Journal of the American College of Cardiology

View full text Add to dashboard Cite

Because a high cardiac death rate is observed in hemodialysis patients, Nishimura et al. (1) evaluated 375 asymptomatic hemodialysis patients with single-photon emission computed tomography (SPECT) imaging and beta-methylp-[123I]-iodophenyl-pentadecanoic acid (BMIPP), reflecting myocardial fatty acid metabolism. Severely reduced BMIPP uptake might be related to repetitive ischemia and thus be predictive for cardiac death. Patients with severely abnormal BMIPP SPECT images had a significantly higher mortality rate at 3 years than those without (39% vs. 2%).The volume of diagnostic imaging has increased exponentially over the recent years, and the appropriateness of these tests has been questioned (2). As a first step in evaluating the appropriateness, Gibbons et al. (3) applied recommended criteria to 284 patients who underwent SPECT myocardial perfusion imaging and found 64% of studies were classified appropriate, 11% were uncertain, 14% were inappropriate, and 11% were unclassifiable; similar numbers were reported for stress echocardiography. Application of these criteria will require refinement.It remains uncertain whether cardiac medications need to be withdrawn before SPECT myocardial perfusion imaging. In a dedicated review, Zoghbi et al. (4) addressed this topic. They emphasized that, when detection of coronary artery disease (CAD) is the question, withdrawal of cardiac medication (e.g., beta-blockers) is appropriate to increase sensitivity of the technique. If the question is whether ischemia remains despite medical therapy, then medication should be continued.The use of nuclear techniques in the field of molecular imaging is increasing. Terrovitis et al. (5) demonstrated in an elegant animal model that ectopic expression of the sodiumiodide symporter enables imaging of transplanted cardiac stem cells with either positron emission tomography or SPECT imaging. Thus far, these techniques are used in animal models but should eventually be suitable for patients (6). Cardiac Computed Tomography (CT)CT calcium scoring and angiography is being increasingly used. In 2008, the feasibility and excellent accuracy for detection of CAD of 256-row detector CT was first reported (7). However, Meijboom et al. (8) have demonstrated that 50% of the atherosclerotic lesions detected on CT angiography do not result in ischemia as demonstrated by normal intracoronary fractional flow reserve. More recently, the focus of CT angiography is shifting toward prognosis (9). Raggi et al. (10) assessed all-cause mortality in 35,388 patients after a mean follow-up of 5.8 Ϯ 3 years. Increasing calcium scores were related to decreasing survival. Importantly, even in patients Ն70 years of age, calcium scoring contributed significantly to risk stratification. Ostrom et al. (11) evaluated 2,538 patients (suspected for CAD) with electron beam CT and demonstrated that CT angiography was an independent predictor of mortality.Choi et al. (12) reported on the potential use of multislice CT for screening asymptomatic individuals for CAD. A total o...

show abstract

Patch Closure of Muscular Ventricular Septal Defects With a New Hybrid Therapy in a Pig Model

Abstract: Here, we present a novel technique for hybrid closure of mVSDs without use of CPB. Further development of the patch system is necessary to assess applicability in humans, especially for the target group of newborns and infants.

Cited by 14 publications

References 23 publications

Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels

Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels

Morphological and Transcriptomic Comparison of Adipose and Bone Marrow Derived Porcine Stem Cells

Highlights of the Year in JACC 2008

Contact Info

Product

Resources

About