Pasteurella organism: Its isolation and identification from pneumonic lungs of goats in Ethiopia

Abstract

Objective: The objective of this study was to isolate and identify Pasteurella spp. associated with pneumonic lungs showing respiratory signs of goats in Ethiopia. Materials and methods: A total of 2400 goats that were slaughtered at the Hashim's Ethiopian Livestock and Meat Export abattoir, Ethiopia were randomly selected for this cross-sectional study during the period of October 2013 to July 2014. Detail ante-mortem, and post-mortem (PM) lesions were inspected, and the suspected samples were collected aseptically from the lungs. Among 2400 goats, 31(1.29%) goats were not slaughtered because these goats showed severe clinical signs. Thus, 2369 goats were slaughtered finally. The collected samples were subjected for isolation and identification of bacterial species following conventional methods such as culture and biochemical examinations. Results: Out of 2400 goats examined, 960(40%) animals showed different abnormal respiratory signs. Based on PM findings, 16.21% (n=384/2369) lungs were found as pneumonic, of which 78.38% (n=301/384) were found to be associated with Pasteurella organism. The overall prevalence of Pasteurella organism (Mannheimia haemolytica and Pasteurella multocida) was 12.71% (n=301/2369). In this study, youngers and goats with medium body condition score (BCS) had greater probability (P<0.05) to be infected by the bacteria though there was no difference in exposure to the organism among goats from Arsi, Bale and Hararghe. On the other hand, out of 301 positive cases, 274(91.03%) were caused by M. haemolytica, and 27(8.97%) were caused by P. multocida isolates. Conclusion: Pasteurella organism especially M. hemolytica is one of the most common causes of pneumonic pasteurellosis in caprine at the study area. So, chemoprophylaxis needs to be given to small ruminants prior to transportation or other stress conditions.

Isolation and Identification of Pasteurella multocida and Mannheimia haemolytica from Pneumonic Small Ruminants and Their Antibiotic Susceptibility in Haramaya District, Eastern Ethiopia

“…Furthermore, this agrees with the findings of Abera et al [ 34 ]; and Ugochukwu [ 30 ]. Conversely, unlike an earlier report by Tesfaye [ 31 ] on lung infection, the prevalence of M. haemolytica isolates in the current study was higher, and it was lower than that of Hailu et al [ 35 ] and Marru et al [ 20 ], who reported 91.03% and 87.5% of M. haemolytica as positive results, respectively. Unlike in our study, M. haemolytica in goats was not prominent in another study [ 36 ].…”

Prevalence of Pneumonia in Sheep and Goats Slaughtered at Elfora Bishoftu Export Abattoir, Ethiopia: A Pathological Investigation

“…when the immune system of the animal is compromised by aforementioned stress factors [4, 42]. The above bacteria and other opportunistic bacteria were isolated from the respiratory tract of healthy and pneumonic sheep and goats in different parts of the country [43–48].…”

Identification of serotypes Pasteurella multocida and Mannheimia haemolytica from cattle and sheep in central Ethiopia

“…Identification of the serotypes involved and production of vaccines against the serotypes prevalent in the country is important in this regard. Although the prevalence of bacterial species in pneumonic animals has been reported previously [22][23][24][25][26][27][28], the focus of those studies was only pneumonic animals. Besides, the previous studies did not identify the serotypes of the bacteria isolated.…”